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Cited by 63Bone Tissue Engineering Materialsbiodegradable polymer synthesis and propertiesElectrospun Nanofibers in Biomedical Applications

Osteoblasts and fibroblasts attachment to poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) film and electrospun scaffolds

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Kaniuk, Łukasz, Krysiak, Zuzanna J., Metwally, Sara, Stachewicz, Urszula·2020·Materials Science and Engineering: C
DOI 10.1016/j.msec.2020.110668
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Abstract

The cellular response is the most crucial in vitro research. Materials' biocompatibility is determined based on cell proliferation and growth. Moreover, the topography of the scaffold surface is the key to enhance cell attachment and anchoring that importantly control further tissue development. Individual cell types have specific preferences regarding the type of surface and its geometry. In our research, we used poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) PHBV to produce two types of substrate: a 3D structure of electrospun fibers and 2D flat films. The PHBV products were morphologically characterized by scanning electron microscopy (SEM). The cytocompatibility was evaluated with cell viability and proliferation using two different types of cells: human osteoblast-like cells (MG-63) and NIH 3 T3 murine fibroblast cells. The behaviour of both cell types was compared on the similar PHBV fiber scaffolds and films using two types of polystyrene (PS) based substrate for the cell culture study: unmodified PS that is not favourable for the attachment of cells and on tissue culture polystyrene (TCPS) plates, which are chemically modify to enhance cells attachment. The results clearly showed high biocompatibility of PHBV as both types of cells showed similar proliferation. These results indicated that PHBV scaffolds are suitable for the development of multifunctional substrates facilitating the growth of different types of tissue regardless of the 3D and 2D designed structures for regeneration purposes.

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Cited by

Sorted by citations

Authors
Kaniuk, Ł., Krysiak, Z. J., Metwally, S., & Stachewicz, U.
Affiliations
AGH University of Krakow (PL)
Corresponding author
Stachewicz, Urszula
ORCID
Venue
Materials Science and Engineering: C
Publisher
Elsevier BV
Published
2020-05
Volume
110
Pages
110668
ISSN
0928-4931 (ISSN-L)
DOI
10.1016/j.msec.2020.110668
OpenAlex
W3000277234
Cited by
63
References
1
Citations by year
2026: 1 · 2025: 7 · 2024: 12 · 2023: 12 · 2022: 13 · 2021: 14 · 2020: 4
FWCI
3.31
Type
journal article
Language
en
Open access
Licence
Locations
  • Materials Science and Engineering C (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Foundation for Polish Science
  • European Union (POIR.04.04.00-00- 4571/18-00)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Keywords
poly(3-hydroxybutyrate-co-3-hydroxyvalerate), electrospun scaffolds, cell attachment, osteoblasts, fibroblasts, biocompatibility, MG-63 cells, NIH3T3 cells, surface topography, tissue culture polystyrene, polystyrene substrate, cell proliferation, tissue regeneration
MeSH terms
Polyhydroxybutyrates, Animals, Cell Survival, Fibroblasts, Osteoblasts, Polyesters, Surface Properties, Tissue Engineering, NIH 3T3 Cells, Cell Shape, Cell Proliferation, Mice, Tissue Scaffolds

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