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Cited by 33Cell death mechanisms and regulationRedox biology and oxidative stressProtein Kinase Regulation and GTPase Signaling

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Role of oxidative stress and JNK pathway in apoptotic death induced by potassium deprivation and staurosporine in cerebellar granule neurons

Ramiro-Cortés, Yazmín, Morán, Julio·2009·Neurochemistry International
DOI 10.1016/j.neuint.2009.05.015
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Abstract

Several signaling pathways are differentially activated during apoptotic cell death. We have previously found that during apoptotic death of cerebellar granule neurons (CGN) induced by potassium deprivation (K5) and staurosporine there is an increase in the generation of reactive oxygen species (ROS). The inhibition of ROS generation reduces the extent of cell death. However, remain to be elucidated the mechanisms by which ROS participate in this apoptotic process. On the other hand, it is well known that c-Jun amino-terminal kinase (JNK) pathway plays a pivotal role in cell death of several cell types. In the present study we found that K5 activated the JNK pathway and that its inhibition with SP600125 markedly prevented caspase 3 activation, nuclear condensation and cell death induced by K5. In contrast, JNK pathway was not activated by staurosporine and the JNK inhibitor did not affect cell death induced by this stimulus. We also found that JNK inhibition did not affect ROS levels induced by K5 or staurosporine, suggesting that ROS are upstream of JNK pathway activation. Antioxidants increased ASK1 phosphorylation and decreased JNK1/2 and c-Jun phosphorylation induced by K5. According to these results, we suggest that apoptosis induced by K5 is JNK-dependent and mediated by ROS, but apoptosis induced by staurosporine is not dependent on JNK and that the observed ROS generation by staurosporine seems not to be involved in the activation of this signaling pathway.

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  1. Alavez, 2003, Mechanisms of cell death by deprivation of depolarizing conditions during cerebellar granule neurons maturation, Neurochem. Int., 43, 581, 10.1016/S0197-0186(03)00079-2 · DOI
  2. Becker, 2004, Characterization of the c-Jun N-terminal kinase-BimEL signaling pathway in neuronal apoptosis, J. Neurosci., 24, 8762, 10.1523/JNEUROSCI.2953-04.2004 · DOI · Paper page
  3. Behrens, 1999, Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation, Nat. Genet., 21, 326, 10.1038/6854 · DOI
  4. Bhat, 1999, Hydrogen peroxide activation of multiple mitogen-activated protein kinases in an oligodendrocyte cell line: role of extracellular signal-regulated kinase in hydrogen peroxide-induced cell death, J. Neurochem., 72, 112, 10.1046/j.1471-4159.1999.0720112.x · DOI
  5. Blomgren, 2007, Pathological apoptosis in the developing brain, Apoptosis, 12, 993, 10.1007/s10495-007-0754-4 · DOI
  6. Bode, 2007, The functional contrariety of JNK, Mol. Carcinog., 46, 591, 10.1002/mc.20348 · DOI
  7. Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  8. Bruckner, 2001, JNK3 contributes to c-Jun activation and apoptosis but not oxidative stress in nerve growth factor-deprived sympathetic neurons, J. Neurochem., 78, 298, 10.1046/j.1471-4159.2001.00400.x · DOI
  9. Bunkoczi, 2007, Structural and functional characterization of the human protein kinase ASK1, Structure, 15, 1215, 10.1016/j.str.2007.08.011 · DOI
  10. Caballero-Benitez, 2003, Caspase activation pathways induced by staurosporine and low potassium: role of caspase-2, J. Neurosci. Res., 71, 383, 10.1002/jnr.10493 · DOI · Paper page
  11. Chang, 2001, Mammalian MAP kinase signalling cascades, Nature, 410, 37, 10.1038/35065000 · DOI
  12. Choi, 2004, Phosphorylation of p38 MAPK induced by oxidative stress is linked to activation of both caspase-8- and -9-mediated apoptotic pathways in dopaminergic neurons, J. Biol. Chem., 279, 20451, 10.1074/jbc.M311164200 · DOI
  13. Christie, 2007, Differential regulation of inhibitors of apoptosis proteins in Alzheimer's disease brains, Neurobiol. Dis., 26, 165, 10.1016/j.nbd.2006.12.017 · DOI
  14. Coffey, 2002, c-Jun N-terminal protein kinase (JNK) 2/3 is specifically activated by stress, mediating c-Jun activation, in the presence of constitutive JNK1 activity in cerebellar neurons, J. Neurosci., 22, 4335, 10.1523/JNEUROSCI.22-11-04335.2002 · DOI · Paper page
  15. Coyoy, 2008, Role of NADPH oxidase in the apoptotic death of cultured cerebellar granule neurons, Free Radic. Biol. Med., 45, 1056, 10.1016/j.freeradbiomed.2008.06.027 · DOI
  16. Cozzolino, 2006, Apaf1 mediates apoptosis and mitochondrial damage induced by mutant human SOD1s typical of familial amyotrophic lateral sclerosis, Neurobiol. Dis., 21, 69, 10.1016/j.nbd.2005.06.010 · DOI
  17. D’Mello, 1997, Insulin-like growth factor and potassium depolarization maintain neuronal survival by distinct pathways: possible involvement of PI 3-kinase in IGF-1 signaling, J. Neurosci., 17, 1548, 10.1523/JNEUROSCI.17-05-01548.1997 · DOI
  18. D’Mello, 1993, Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP, Proc. Natl. Acad. Sci. U.S.A., 90, 10989, 10.1073/pnas.90.23.10989 · DOI · Paper page
  19. Davis, 2000, Signal transduction by the JNK group of MAP kinases, Cell, 103, 239, 10.1016/S0092-8674(00)00116-1 · DOI · Paper page
  20. Ferri, 2003, Vitamin E affects cell death in adult rat dentate gyrus, J. Neurocytol., 32, 1155, 10.1023/B:NEUR.0000021909.84327.e8 · DOI
  21. Fujino, 2006, Thioredoxin and protein kinases in redox signaling, Semin. Cancer Biol., 16, 427, 10.1016/j.semcancer.2006.09.003 · DOI
  22. Gallo, 1987, The role of depolarization in the survival and differentiation of cerebellar granule cells in culture, J. Neurosci., 7, 2203, 10.1523/JNEUROSCI.07-07-02203.1987 · DOI
  23. Gil, 2003, Cytosolic and mitochondrial ROS in staurosporine-induced retinal cell apoptosis, Free Radic. Biol. Med., 35, 1500, 10.1016/j.freeradbiomed.2003.08.022 · DOI
  24. Goldman, 2004, Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation, J. Biol. Chem., 279, 10442, 10.1074/jbc.M311129200 · DOI
  25. Gotoh, 1998, Reactive oxygen species- and dimerization-induced activation of apoptosis signal-regulating kinase 1 in tumor necrosis factor-alpha signal transduction, J. Biol. Chem., 273, 17477, 10.1074/jbc.273.28.17477 · DOI
  26. Greenlund, 1995, Superoxide dismutase delays neuronal apoptosis: a role for reactive oxygen species in programmed neuronal death, Neuron, 14, 303, 10.1016/0896-6273(95)90287-2 · DOI
  27. Harada, 1999, An inhibitor of p38 and JNK MAP kinases prevents activation of caspase and apoptosis of cultured cerebellar granule neurons, Jpn. J. Pharmacol., 79, 369, 10.1254/jjp.79.369 · DOI
  28. Harris, 2002, Identification of JNK-dependent and -independent components of cerebellar granule neuron apoptosis, J. Neurochem., 83, 992, 10.1046/j.1471-4159.2002.01219.x · DOI
  29. Hoeflich, 1999, Mediation of TNF receptor-associated factor effector functions by apoptosis signal-regulating kinase-1 (ASK1), Oncogene, 18, 5814, 10.1038/sj.onc.1202975 · DOI
  30. Jiang, 1996, Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta), J. Biol. Chem., 271, 17920, 10.1074/jbc.271.30.17920 · DOI
  31. Jiang, 1997, Characterization of the structure and function of the fourth member of p38 group mitogen-activated protein kinases, p38delta, J. Biol. Chem., 272, 30122, 10.1074/jbc.272.48.30122 · DOI
  32. Kaufmann, 2001, Programmed cell death: alive and well in the new millennium, Trends Cell Biol., 11, 526, 10.1016/S0962-8924(01)02173-0 · DOI
  33. Krohn, 1998, Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector, J. Neurosci., 18, 8186, 10.1523/JNEUROSCI.18-20-08186.1998 · DOI
  34. Kruman, 1998, Calcium and reactive oxygen species mediate staurosporine-induced mitochondrial dysfunction and apoptosis in PC12 cells, J. Neurosci. Res., 51, 293, 10.1002/(SICI)1097-4547(19980201)51:3<293::AID-JNR3>3.0.CO;2-B · DOI
  35. Kyriakis, 2001, Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation, Physiol. Rev., 81, 807, 10.1152/physrev.2001.81.2.807 · DOI
  36. Lang, 2004, Cooperation between PKC-alpha and PKC-epsilon in the regulation of JNK activation in human lung cancer cells, Cell. Signal., 16, 457, 10.1016/j.cellsig.2003.09.002 · DOI
  37. Lopez-Bergami, 2005, RACK1 mediates activation of JNK by protein kinase C [corrected], Mol. Cell., 19, 309, 10.1016/j.molcel.2005.06.025 · DOI
  38. Lopez-Bergami, 2008, Requirements for PKC-augmented JNK activation by MKK4/7, Int. J. Biochem. Cell. Biol., 40, 1055, 10.1016/j.biocel.2007.11.011 · DOI
  39. Luo, 2003, Akt as a mediator of cell death, Proc. Natl. Acad. Sci. U.S.A., 100, 11712, 10.1073/pnas.1634990100 · DOI
  40. Macdonald, 2003, Tumour necrosis factor-alpha- vs. growth factor deprivation-promoted cell death: distinct converging pathways, Aging Cell, 2, 245, 10.1046/j.1474-9728.2003.00059.x · DOI
  41. Martin, 1999, Neuronal death in amyotrophic lateral sclerosis is apoptosis: possible contribution of a programmed cell death mechanism, J. Neuropathol. Exp. Neurol., 58, 459, 10.1097/00005072-199905000-00005 · DOI
  42. Martin-Romero, 2002, Inhibition of oxidative stress produced by plasma membrane NADH oxidase delays low-potassium-induced apoptosis of cerebellar granule cells, J. Neurochem., 82, 705, 10.1046/j.1471-4159.2002.01023.x · DOI
  43. Miller, 1996, Metabolic and genetic analyses of apoptosis in potassium/serum-deprived rat cerebellar granule cells, J. Neurosci., 16, 7487, 10.1523/JNEUROSCI.16-23-07487.1996 · DOI · Paper page
  44. Moran, 1999, Caspase-3 expression by cerebellar granule neurons is regulated by calcium and cyclic AMP, J. Neurochem., 73, 568, 10.1046/j.1471-4159.1999.0730568.x · DOI
  45. Moran, 1989, Effect of potassium depolarization on phosphate-activated glutaminase activity in primary cultures of cerebellar granule neurons and astroglial cells during development, Brain Res. Dev. Brain Res., 46, 97, 10.1016/0165-3806(89)90146-6 · DOI
  46. Nakaso, 2008, Caffeine activates the PI3K/Akt pathway and prevents apoptotic cell death in a Parkinson's disease model of SH-SY5Y cells, Neurosci. Lett., 432, 146, 10.1016/j.neulet.2007.12.034 · DOI
  47. Nardi, 1997, Biochemical and temporal analysis of events associated with apoptosis induced by lowering the extracellular potassium concentration in mouse cerebellar granule neurons, J. Neurochem., 68, 750, 10.1046/j.1471-4159.1997.68020750.x · DOI
  48. Oh, 2006, AIF translocates to the nucleus in the spinal motor neurons in a mouse model of ALS, Neurosci. Lett., 406, 205, 10.1016/j.neulet.2006.07.044 · DOI
  49. Oppenheim, 1991, Cell death during development of the nervous system, Annu. Rev. Neurosci., 14, 453, 10.1146/annurev.ne.14.030191.002321 · DOI · Paper page
  50. Ortner, 2007, Heteromeric complex formation of ASK2 and ASK1 regulates stress-induced signaling, Biochem. Biophys. Res. Commun., 362, 454, 10.1016/j.bbrc.2007.08.006 · DOI
  51. Pimienta, 2007, Canonical and alternative MAPK signaling, Cell Cycle, 6, 2628, 10.4161/cc.6.21.4930 · DOI
  52. Prehn, 1997, Ca2+ and reactive oxygen species in staurosporine-induced neuronal apoptosis, J. Neurochem., 68, 1679, 10.1046/j.1471-4159.1997.68041679.x · DOI
  53. Pulverer, 1991, Phosphorylation of c-jun mediated by MAP kinases, Nature, 353, 670, 10.1038/353670a0 · DOI
  54. Saitoh, 1998, Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1, EMBO J., 17, 2596, 10.1093/emboj/17.9.2596 · DOI · Paper page
  55. Sajan, 2007, Apoptotic gene expression in Alzheimer's disease hippocampal tissue., Am. J. Alzheimers Dis. Other Demen., 22, 319, 10.1177/1533317507302447 · DOI
  56. Samhan-Arias, 2004, Kaempferol blocks oxidative stress in cerebellar granule cells and reveals a key role for reactive oxygen species production at the plasma membrane in the commitment to apoptosis, Free Radic. Biol. Med., 37, 48, 10.1016/j.freeradbiomed.2004.04.002 · DOI
  57. Schroeter, 2001, Flavonoids protect neurons from oxidized low-density-lipoprotein-induced apoptosis involving c-Jun N-terminal kinase (JNK), c-Jun and caspase-3, Biochem. J., 358, 547, 10.1042/0264-6021:3580547 · DOI
  58. Schulz, 1996, Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species, J. Neurosci., 16, 4696, 10.1523/JNEUROSCI.16-15-04696.1996 · DOI
  59. Tatton, 2003, Apoptosis in Parkinson's disease: signals for neuronal degradation, Ann. Neurol., 53, S61, 10.1002/ana.10489 · DOI
  60. Thornberry, 1997, A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis, J. Biol. Chem., 272, 17907, 10.1074/jbc.272.29.17907 · DOI
  61. Tibbles, 1999, The stress-activated protein kinase pathways, Cell. Mol. Life Sci., 55, 1230, 10.1007/s000180050369 · DOI
  62. Tobiume, 2001, ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis, EMBO Rep., 2, 222, 10.1093/embo-reports/kve046 · DOI
  63. Tobiume, 2002, Activation of apoptosis signal-regulating kinase 1 by the stress-induced activating phosphorylation of pre-formed oligomer, J. Cell. Physiol., 191, 95, 10.1002/jcp.10080 · DOI
  64. Tran, 2007, kinase delays caspase-9 activation by interaction with the apoptosome, J. Biol. Chem., 282, 20340, 10.1074/jbc.M702210200 · DOI
  65. Valencia, 2001, Role of oxidative stress in the apoptotic cell death of cultured cerebellar granule neurons, J. Neurosci. Res., 64, 284, 10.1002/jnr.1077 · DOI
  66. Valencia, 2004, Reactive oxygen species induce different cell death mechanisms in cultured neurons, Free Radic. Biol. Med., 36, 1112, 10.1016/j.freeradbiomed.2004.02.013 · DOI
  67. Wang, 2003, Regulation of c-Jun N-terminal kinase activation in hydrogen peroxide induced neurotoxicity, J. Neurocytol., 32, 143, 10.1023/B:NEUR.0000005599.10952.33 · DOI
  68. Watson, 1998, Phosphorylation of c-Jun is necessary for apoptosis induced by survival signal withdrawal in cerebellar granule neurons, J. Neurosci., 18, 751, 10.1523/JNEUROSCI.18-02-00751.1998 · DOI
  69. Widmann, 1999, Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human, Physiol. Rev., 79, 143, 10.1152/physrev.1999.79.1.143 · DOI
  70. Wu, 2005, Apoptotic signals within the basal forebrain cholinergic neurons in Alzheimer's disease, Exp. Neurol., 195, 484, 10.1016/j.expneurol.2005.06.020 · DOI
  71. Xifro, 2006, N-methyl-d-aspartate blocks activation of JNK and mitochondrial apoptotic pathway induced by potassium deprivation in cerebellar granule cells, J. Biol. Chem., 281, 6801, 10.1074/jbc.M504571200 · DOI
  72. Yamagishi, 2003, Comparison of inhibitory effects of brain-derived neurotrophic factor and insulin-like growth factor on low potassium-induced apoptosis and activation of p38 MAPK and c-Jun in cultured cerebellar granule neurons, Brain Res. Mol. Brain Res., 119, 184, 10.1016/j.molbrainres.2003.09.009 · DOI
  73. Zhang, 1999, Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 proteins, Proc. Natl. Acad. Sci. U.S.A., 96, 8511, 10.1073/pnas.96.15.8511 · DOI
  74. Zhou, 2007, Superoxide dismutase/catalase mimetics but not MAP kinase inhibitors are neuroprotective against oxygen/glucose deprivation-induced neuronal death in hippocampus, J. Neurochem., 103, 2212, 10.1111/j.1471-4159.2007.04906.x · DOI
  75. Zhou, 2008, c-Jun N-terminal kinase regulates mitochondrial bioenergetics by modulating pyruvate dehydrogenase activity in primary cortical neurons, J. Neurochem., 104, 325, 10.1111/j.1471-4159.2007.04957.x · DOI

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Metadata

Authors
Ramiro-Cortés, Y., & Morán, J.
Affiliations
Universidad Nacional Autónoma de México (MX)
Corresponding author
Morán, Julio
ORCID
Morán, Julio
Venue
Neurochemistry International
Publisher
Elsevier BV
Published
2009-12
Volume
55
Issue
7
Pages
581-592
ISSN
0197-0186 (ISSN-L)
DOI
10.1016/j.neuint.2009.05.015
OpenAlex
W2014294777
Cited by
33
References
2
Citations by year
2024: 2 · 2023: 1 · 2021: 2 · 2020: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 4 · 2013: 4 · 2012: 1
FWCI
1.74
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neurochemistry International (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
oxidative stress, JNK signaling, apoptosis, potassium deprivation, staurosporine, cerebellar granule cells, reactive oxygen species, ASK1, c-Jun phosphorylation, caspase-3, SP600125
MeSH terms
Animals, Antioxidants, Cell Nucleus, Cell Survival, Cerebellum, Enzyme Inhibitors, Glyceraldehyde-3-Phosphate Dehydrogenases, Hypokalemia, Neurons, Blotting, Western, Signal Transduction, Rats, Wistar, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Staurosporine, Kaempferols, JNK Mitogen-Activated Protein Kinases, Rats, Caspase 3

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Ramiro-Cortés, Y., & Morán, J. (2009). Role of oxidative stress and JNK pathway in apoptotic death induced by potassium deprivation and staurosporine in cerebellar granule neurons. Neurochemistry International. https://doi.org/10.1016/j.neuint.2009.05.015