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Astrocyte culture models: Molecular and function characterization of primary culture, immortalized astrocytes and C6 glioma cells

Galland, Fabiana, Seady, Marina, Taday, Jessica, Smaili, Soraya Soubhi, Gonçalves, Carlos Alberto, Leite, Marina Concli·2019·Neurochemistry International
DOI 10.1016/j.neuint.2019.104538
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Abstract

The understanding of the physiology of astrocytes and their role in brain function progresses continuously. Primary astrocyte culture is an alternative method to study these cells in an isolated system: in their physiologic and pathologic states. Cell lines are often used as an astrocyte model, since they are easier and faster to manipulate and cost less. However, there are a few studies evaluating the different features of these cells which may put into question the validity of using them as astrocyte models. The aim of this study was to compare primary cultures (PC) with two cell lines - immortalized astrocytes and C6 cells, in terms of protein characterization, morphology and metabolic functional activity. Our results showed, under the same culture condition, that immortalized astrocytes and C6 are positive for differentiated astrocytic markers (eg. GFAP, S100B, AQP4 and ALDH1L1), although expressing them in less quantities then primary astrocyte cultures. Glutamate metabolism and cell communication are reduced in proliferative cells. However, glucose uptake is elevated in C6 lineage cells in comparison with primary astrocytes, probably due to their tumorigenic origin and high proliferation rate. Immortalized astrocytes presented a lower growth rate than C6 cells, and a similar basal morphology as primary astrocytes. However, they did not prove to be as good reproductive models of some of the classic astrocytic functions, such as S100B secretion and GFAP content, especiall…

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  • Higher passage C6 cells tend to display more astrocyte-like characteristics, including functional responses that closely parallel those of primary astrocytes.

    “Second, higher passage C6 cells tend to display more astrocyte‐like characteristics, including functional responses that closely parallel those of primary astrocytes (Galland et al. 2019; Lee et al. 1992), thereby supporting their validity for studying cellular responses to LDL cholesterol.”

    From LDL Cholesterol Modulates Astrocyte Metabolism, Lipid Handling, and Morphology: Evidence From In Vitro and In Vivo Models (2026) · cites this paper as Galland et al. 2019

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Metadata

Authors
Galland, F., Seady, M., Taday, J., Smaili, S. S., Gonçalves, C. A., & Leite, M. C.
Venue
Neurochemistry International
Publisher
Elsevier BV
Published
2019-12
Volume
131
Pages
104538
ISSN
0197-0186
DOI
10.1016/j.neuint.2019.104538
Cited by
121
References
2
Language
en
Licence
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
  • Instituto Nacional de Ciência e tecnologia

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