Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis
Abstract
Probiotics participate actively in the neuropsychiatric disorders. However, their roles on ischemic stroke remain unclear. This study aims to determine whether Clostridium butyricum (C. butyricum) could attenuate cerebral ischemia/reperfusion (I/R) injury and its possible mechanisms. Male ICR mice were intragastrically pretreated with C. butyricum for 2 successive weeks, and then subjected to cerebral I/R injury induced by the bilateral common carotid artery occlusion (BCCAO) for 20min. After 24h of the reperfusion, neurological deficit scores were evaluated. Histopathological changes of the hippocampus neurons were observed using Hematoxylin and eosin (H&E) and TUNEL staining. Malondialdehyde (MDA) contents and superoxide dismutase (SOD) activities in the brain were detected. The expression of Caspase-3, Bax and Bcl-2 were investigated by Western blot and immunohistochemistry analysis. The butyrate contents in the brain were determined. Our results showed that cerebral I/R injury led to neurological deficit, increased levels of Caspase-3 and Bax and decreased Bcl-2/Bax ratio. C. butyricum significantly improved neurological deficit, relieved histopathologic change, decreased MDA contents and increased SOD activities in the I/R injury mice. After C. butyricum pretreatment, the expression of Caspase-3 and Bax were significantly decreased, the Bcl-2/Bax ratio was significantly increased, and butyrate contents in the brain were significantly increased. These findings suggested t…
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Levels of butyrate in the brain of mice supplemented with live Clostridium butyricum reached 0.4 to 0.7 μmol/g, about an order of magnitude higher than concentrations reported in peripheral blood.
“the levels of butyrate in the brain of mice supplemented with live Clostridium butyricum reached a range from 0.4 to 0.7 μmol/g, which was about an order of magnitude higher than concentrations reported in peripheral blood (96, 97).”
From The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication (2020) · cites this paper as [97]
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Savignac, H. M., Kiely, B., Dinan, T. G., & Cryan, J. F. (2014) · Neurogastroenterology & Motility · doi:10.1111/nmo.12427 · Cited by 418 · see citing works
Microbes and the gut‐brain axis
Bercik, P., Collins, S. M., & Verdu, E. F. (2012) · Neurogastroenterology & Motility · doi:10.1111/j.1365-2982.2012.01906.x · Cited by 417 · see citing works
Liu, J., Sun, J., Wang, F., Yu, X., Ling, Z., Li, H., Zhang, H., Jin, J., Chen, W., Pang, M., et al. (2015) · BioMed Research International · doi:10.1155/2015/412946 · Cited by 225 · see citing works
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Cryan, J. F., O'Riordan, K. J., Cowan, C. S. M., Sandhu, K. V., Bastiaanssen, T. F. S., Boehme, M., Codagnone, M. G., Cussotto, S., Fulling, C., Golubeva, A. V., et al. (2019) · Physiological Reviews · doi:10.1152/physrev.00018.2018 · Cited by 5,286 · see citing works
The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication
Silva, Y. P., Bernardi, A., & Frozza, R. L. (2020) · Frontiers in Endocrinology · doi:10.3389/fendo.2020.00025 · Cited by 3,043 · see citing works
“an order of magnitude higher than concentrations reported in peripheral blood (96, 97).”
BackgroundThe neuropharmacology of butyrate: the bread and butter of the microbiota-gut-brain axis
Stilling, R. M., van de Wouw, M., Clarke, G., Stanton, C., Dinan, T. G., & Cryan, J. F. (2016) · Neurochemistry International · doi:10.1016/j.neuint.2016.06.011 · Cited by 891 · see citing works
Autism Spectrum Disorders and the Gut Microbiota
Fattorusso, A., Di Genova, L., Dell’Isola, G., Mencaroni, E., & Esposito, S. (2019) · Nutrients · doi:10.3390/nu11030521 · Cited by 484 · see citing works
Sun, J., Xu, J., Yang, B., Chen, K., Kong, Y., Fang, N., Gong, T., Wang, F., Ling, Z., & Liu, J. (2020) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201900636 · Cited by 302 · see citing works
Self-citationMicrobes, immunity, and behavior: psychoneuroimmunology meets the microbiome
Dinan, T. G., & Cryan, J. F. (2017) · Neuropsychopharmacology · doi:10.1038/npp.2016.103 · Cited by 246 · see citing works
Xiao, W., Su, J., Gao, X., Yang, H., Weng, R., Ni, W., & Gu, Y. (2022) · Microbiome · doi:10.1186/s40168-022-01255-6 · Cited by 252 · see citing works
Liu, J., Wang, F., Liu, S., Du, J., Hu, X., Xiong, J., Fang, R., Chen, W., & Sun, J. (2017) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2017.08.3235 · Cited by 218 · see citing works
Self-citationSpichak, S., Bastiaanssen, T. F. S., Berding, K., Vlckova, K., Clarke, G., Dinan, T. G., & Cryan, J. F. (2021) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2021.02.044 · Cited by 127 · see citing works
Zhao, L., Xiong, Q., Stary, C. M., Mahgoub, O. K., Ye, Y., Gu, L., Xiong, X., & Zhu, S. (2018) · Journal of Neuroinflammation · doi:10.1186/s12974-018-1382-3 · Cited by 113 · see citing works
Tian, Z., Tang, C., & Wang, Z. (2019) · Journal of Cellular Biochemistry · doi:10.1002/jcb.29169 · Cited by 57 · see citing works
- Authors
- Sun, J., Ling, Z., Wang, F., Chen, W., Li, H., Jin, J., Zhang, H., Pang, M., Yu, J., & Liu, J.
- Affiliations
- Wenzhou Medical University (CN)
- First Affiliated Hospital Zhejiang University (CN)
- Corresponding author
- Sun, Jing
- Ling, Zongxin
- Liu, Jiaming
- ORCID
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2016-02
- Volume
- 613
- Pages
- 30-35
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2228779853
- Cited by
- 183
- References
- 3
- Citations by year
- 2026: 7 · 2025: 23 · 2024: 14 · 2023: 23 · 2022: 32 · 2021: 29 · 2020: 15 · 2019: 16 · 2018: 6 · 2017: 6 · 2016: 12
- FWCI
- 4.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Zhejiang Medical Science and Technology (2016136369)
- Zhejiang Science and Technology Development (2015C37010)
- Topics
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- Clostridium butyricum, cerebral ischemia-reperfusion injury, ischemic stroke, probiotics, neuroprotection, oxidative stress, apoptosis, butyrate, caspase-3, Bax, Bcl-2, malondialdehyde, superoxide dismutase, hippocampus, bilateral common carotid artery occlusion, neurological deficit
- MeSH terms
- Animals, Brain, Brain Ischemia, Male, Mice, Inbred ICR, Neurons, Reperfusion Injury, Carotid Stenosis, Apoptosis, Carotid Artery, Common, Oxidative Stress, Proto-Oncogene Proteins c-bcl-2, Probiotics, Clostridium butyricum, bcl-2-Associated X Protein, Caspase 3
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