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Cited by 47Advanced Neuroimaging Techniques and ApplicationsAdvanced MRI Techniques and ApplicationsNMR spectroscopy and applications

Cytoarchitecture of the mouse brain by high resolution diffusion magnetic resonance imaging

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Wang, Nian, White, Leonard E., Qi, Yi, Cofer, Gary, Johnson, G. Allan·2020·NeuroImage
DOI 10.1016/j.neuroimage.2020.116876PMC PMC7299741
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Abstract

MRI has been widely used to probe the neuroanatomy of the mouse brain, directly correlating MRI findings to histology is still challenging due to the limited spatial resolution and various image contrasts derived from water relaxation or diffusion properties. Magnetic resonance histology has the potential to become an indispensable research tool to mitigate such challenges. In the present study, we acquired high spatial resolution MRI datasets, including diffusion MRI (dMRI) at 25 ​μm isotropic resolution and quantitative susceptibility mapping (QSM) at 21.5 ​μm isotropic resolution to validate with conventional mouse brain histology. Diffusion weighted images (DWIs) show better delineation of cortical layers and glomeruli in the olfactory bulb than fractional anisotropy (FA) maps. However, among all the image contrasts, including quantitative susceptibility mapping (QSM), T1/T2∗ images and DTI metrics, FA maps highlight unique laminar architecture in sub-regions of the hippocampus, including the strata of the dentate gyrus and CA fields of the hippocampus. The mean diffusivity (MD) and axial diffusivity (AD) yield higher correlation with DAPI (0.62 and 0.71) and NeuN (0.78 and 0.74) than with NF-160 (-0.34 and -0.49). The correlations between FA and DAPI, NeuN, and NF-160 are 0.31, -0.01, and -0.49, respectively. Our findings demonstrate that MRI at microscopic resolution deliver a three-dimensional, non-invasive and non-destructive platform for characterization of fine structural detail in both gray matter and white matter of the mouse brain.

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  • Long data acquisitions possible in ex vivo DTI allow high spatial resolution without image corruption due to motion or breathing that are common in in vivo DTI.

    “We leveraged the ability of long data acquisitions that are possible in ex vivo DTI to achieve a high spatial resolution, without suffering from image corruption due to motion or breathing that are common in in vivo DTI [12,13].”

    From Diffusion tensor imaging reveals whole-brain microstructural changes in the P301L mouse model of tauopathy. (2020) · cites this paper as [12]

    The Methods (MRH protocols; page not provided) report a 95.3-hour diffusion MRI acquisition at 25 μm isotropic resolution, supporting the long-acquisition, high-resolution portion of the claim. The passages do not address whether ex vivo imaging avoids corruption from motion or breathing, or whether those artifacts are common in in vivo DTI. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Diffusion-weighted imaging probes the directional diffusivity of water molecules and can yield information about the microstructural properties of brain tissue.

    “Diffusion-weighted imaging is a MRI technique that probes the directional diffusivity of water molecules and can, thus, yield information about the microstructural properties of brain tissue [11][12][13].”

    From Diffusion tensor imaging reveals whole-brain microstructural changes in the P301L mouse model of tauopathy. (2020) · cites this paper as [12]

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Cited by

Sorted by citations

Authors
Wang, N., White, L. E., Qi, Y., Cofer, G., & Johnson, G. A.
Affiliations
  • Duke University (US)
  • Duke Medical Center (US)
Corresponding author
  • Wang, Nian
  • Johnson, G. Allan
ORCID
Venue
NeuroImage
Publisher
Elsevier BV
Published
2020-08
Volume
216
Pages
116876
ISSN
1053-8119 (ISSN-L)
DOI
10.1016/j.neuroimage.2020.116876
PMC
PMC7299741
OpenAlex
W3018850970
Cited by
47
References
5
Citations by year
2026: 3 · 2025: 12 · 2024: 4 · 2023: 10 · 2022: 10 · 2021: 6 · 2020: 1 · 2013: 1
FWCI
3.29
Type
journal article
Language
en
Open access
Licence
Locations
  • NeuroImage (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • NIH
  • NIBIB
  • Duke University School of Medicine
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
  • Physical Sciences › Physics and Astronomy › Nuclear and High Energy Physics › NMR spectroscopy and applications
Keywords
mouse brain, diffusion MRI, quantitative susceptibility mapping, fractional anisotropy, dentate gyrus, olfactory bulb glomeruli, mean diffusivity, axial diffusivity, high-resolution MRI
MeSH terms
Animals, Male, Mice, Inbred C57BL, Diffusion Magnetic Resonance Imaging, Mice, Diffusion Tensor Imaging, White Matter, Gray Matter

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