A role for SNAP25 in internalization of kainate receptors and synaptic plasticity
Open accessAbstract
Regulation of surface insertion and internalization of AMPA and NMDA receptors has emerged as a key mechanism for the control of synaptic strength. Regulatory elements for synaptic kainate receptors (KARs) are, however, largely undetermined. We have found that SNAP25 is critical for the synaptic removal of KARs, acting via GluK5 (i.e., KA2) subunits. SNAP25 coimmunoprecipitates with protein complexes containing PICK1, GRIP1, and GluK5 and colocalizes with GluK5 in both hippocampal neurons and transfected HEK293 cells. In hippocampal slices, purified SNAP25 antibodies and blocking peptides caused a GluK5-dependent run-up of KARs-mediated EPSC (EPSC(KAR)) recorded from CA3 pyramidal neurons when included in the patch pipette and prevented activity-dependent long-term depression of EPSC(KAR). As EPSC(KAR) LTD, SNAP25/PICK1/GluK5 interactions are dynamically regulated by PKC.
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SNAP-25 has recently been shown to facilitate the removal of kainate receptors (KARs) from the membrane surface.
“Moreover, SNAP-25 has recently been shown to facilitate the removal of kainate receptors (KARs), which are implicated in epileptogenesis, from the membrane surface (83, 90 -95).”
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Contractor, A., Mulle, C., & Swanson, G. T. (2011) · Trends in Neurosciences · doi:10.1016/j.tins.2010.12.002 · Cited by 292 · see citing works
SNAP-25 is a target of protein kinase C phosphorylation critical to NMDA receptor trafficking
Lau, C. G., Takayasu, Y., Rodenas-Ruano, A., Paternain, A. V., Lerma, J., Bennett, M. V. L., & Zukin, R. S. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.4933-08.2010 · Cited by 120 · see citing works
Self-citationNiere, F., Namjoshi, S., Song, E., Dilly, G. A., Schoenhard, G., Zemelman, B. V., Mechref, Y., & Raab-Graham, K. F. (2016) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m115.055079 · Cited by 38 · see citing works
- Authors
- Selak, S., Paternain, A. V., Aller, I. M., Picó, E., Rivera, R., & Lerma, J.
- Affiliations
- Instituto de Neurociencias (ES)
- Corresponding author
- Lerma, Juan
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2009-08
- Volume
- 63
- Issue
- 3
- Pages
- 357-371
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2078288469
- Cited by
- 95
- References
- 1
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 5 · 2017: 9 · 2016: 4 · 2015: 5 · 2014: 9 · 2013: 10 · 2012: 8
- FWCI
- 3.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1482 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministry of Education and Science (2007-0023, BFI2003-00161, BFU2006-007138)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- SNAP-25, kainate receptors, receptor internalization, synaptic plasticity, PICK1, GRIP1, long-term depression, EPSC, hippocampus, CA3 pyramidal cells, protein kinase C, synaptic regulation
- MeSH terms
- Animals, Animals, Newborn, Calcium, Carrier Proteins, Cell Line, Transformed, Electric Stimulation, Enzyme Inhibitors, Hippocampus, Humans, Luminescent Proteins, Mice, Inbred C57BL, Naphthalenes, Nerve Tissue Proteins, Neural Pathways, Neuroblastoma, Neuronal Plasticity, Neurons, Neurotoxins, Nuclear Proteins, Pyridines, Synapses, Transfection, Kainic Acid Receptors, Receptors, Kainic Acid, Mice, Knockout, Patch-Clamp Techniques, Excitatory Amino Acid Antagonists, Cell Cycle Proteins, Excitatory Postsynaptic Potentials, Protein Transport, Cell Line, Tumor, Immunoprecipitation, Adaptor Proteins, Signal Transducing, Synaptosomal-Associated Protein 25, Vesicle-Associated Membrane Protein 2, Mice, Rats
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