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Microfluidic Local Perfusion Chambers for the Visualization and Manipulation of Synapses
Open accessAbstract
Summary The polarized nature of neurons as well as the size and density of synapses complicates the manipulation and visualization of cell biological processes that control synaptic function. Here we developed a microfluidic local perfusion (μLP) chamber to access and manipulate synaptic regions and pre- and post-synaptic compartments in vitro. This chamber directs the formation of synapses in >100 parallel rows connecting separate neuron populations. A perfusion channel transects the parallel rows allowing access to synaptic regions with high spatial and temporal resolution. We used this chamber to investigate synapse-to-nucleus signaling. Using the calcium indicator dye, Fluo-4, we measured changes in calcium at dendrites and somata, following local perfusion of glutamate. Exploiting the high temporal resolution of the chamber, we exposed synapses to “spaced” or “massed” application of glutamate and then examined levels of pCREB in somata. Lastly, we applied the metabotropic receptor agonist, DHPG, to dendrites and observed increases in Arc transcription and Arc transcript localization.
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Self-citationAshrafi, G., Schlehe, J. S., LaVoie, M. J., & Schwarz, T. L. (2014) · Journal of Cell Biology · doi:10.1083/jcb.201401070 · Cited by 502
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Metadata
- Authors
- Taylor, A. M., Dieterich, D. C., Ito, H. T., Kim, S. A., & Schuman, E. M.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2010-04
- Volume
- 66
- Issue
- 1
- Pages
- 57-68
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2011876206
- Cited by
- 292
- References
- 2
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Taylor, A. M., Dieterich, D. C., Ito, H. T., Kim, S. A., & Schuman, E. M. (2010). Microfluidic Local Perfusion Chambers for the Visualization and Manipulation of Synapses. Neuron. https://doi.org/10.1016/j.neuron.2010.03.022
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