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Cited by 168Axon Guidance and Neuronal SignalingNeurobiology and Insect Physiology ResearchNeurogenesis and neuroplasticity mechanisms

Integrins Regulate Repulsion-Mediated Dendritic Patterning of Drosophila Sensory Neurons by Restricting Dendrites in a 2D Space

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Han, Chun, Wang, Denan, Soba, Peter, Zhu, Sijun, Lin, Xinhua, Jan, Lily Yeh, Jan, Yuh-Nung·2012·Neuron
DOI 10.1016/j.neuron.2011.10.036PMC PMC3269260
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Abstract

Dendrites of the same neuron usually avoid each other. Some neurons also repel similar neurons through dendrite-dendrite interaction to tile the receptive field. Nonoverlapping coverage based on such contact-dependent repulsion requires dendrites to compete for limited space. Here we show that Drosophila class IV dendritic arborization (da) neurons, which tile the larval body wall, grow their dendrites mainly in a 2D space on the extracellular matrix (ECM) secreted by the epidermis. Removing neuronal integrins or blocking epidermal laminin production causes dendrites to grow into the epidermis, suggesting that integrin-laminin interaction attaches dendrites to the ECM. We further show that some of the previously identified tiling mutants fail to confine dendrites in a 2D plane. Expansion of these mutant dendrites in three dimensions results in overlap of dendritic fields. Moreover, overexpression of integrins in these mutant neurons effectively reduces dendritic crossing and restores tiling, revealing an additional mechanism for tiling.

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Authors
Han, C., Wang, D., Soba, P., Zhu, S., Lin, X., Jan, L. Y., & Jan, Y.-N.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Cincinnati Children's Hospital Medical Center (US)
Corresponding author
Jan, Yuh-Nung
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
2012-01
Volume
73
Issue
1
Pages
64-78
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/j.neuron.2011.10.036
PMC
PMC3269260
OpenAlex
W2047448689
Cited by
168
References
1
Citations by year
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FWCI
6.06
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • American Cancer Society (RSG-07-051)
  • California Institute for Regenerative Medicine
  • Jane Coffin Childs Memorial Fund for Medical Research
  • Chinese Academy of Sciences (2R37NS040929, KSCX2-YW-R-263)
  • National Institutes of Health (2R01 GM063891)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
dendritic branching, integrins, laminin, extracellular matrix, Drosophila, dendritic growth, epidermis
MeSH terms
Animals, Cloning, Molecular, Dendrites, Drosophila, Embryo, Nonmammalian, Extracellular Matrix, Laminin, Mutation, Sensory Receptor Cells, Sense Organs, Signal Transduction, Integrins, Gene Expression Regulation, Developmental, Drosophila Proteins, Animals, Genetically Modified, RNA Interference, Microscopy, Electron, Transmission, Neuroimaging

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