The DOI matches its Crossref record.
Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Open accessAbstract
Summary Monitoring neuronal electrical activity using fluorescent protein-based voltage sensors has been limited by small response magnitudes and slow kinetics of existing probes. Here we report the development of a novel fluorescent protein voltage sensor, named ArcLight, and derivative probes that exhibit large changes in fluorescence intensity in response to voltage changes. ArcLight consists of the voltage-sensing domain of Ciona intestinalis voltage-sensitive phosphatase and super ecliptic pHluorin that carries the point mutation A227D. The fluorescence intensity of ArcLight A242 decreases 35% in response to +100mV depolarization when measured in HEK 293 cells, which is more than five times larger than the signals from previously reported fluorescent protein voltage sensors. We show that the combination of signal size and response speed of these new probes, for the first time, allows the reliable detection of single action potentials and excitatory potentials in individual neurons and dendrites.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Daigle, T. L., Madisen, L., Hage, T. A., Valley, M. T., Knoblich, U., Larsen, R. S., Takeno, M. M., Huang, L., Gu, H., Larsen, R., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.035 · Cited by 817
High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor
Gong, Y., Huang, C., Li, J. Z., Grewe, B. F., Zhang, Y., Eismann, S., & Schnitzer, M. J. (2015) · Science · doi:10.1126/science.aab0810 · Cited by 462
Knecht, Z. A., Silbering, A. F., Ni, L., Klein, M., Budelli, G., Bell, R., Abuin, L., Ferrer, A. J., Samuel, A. D., Benton, R., et al. (2016) · eLife · doi:10.7554/elife.17879 · Cited by 258
Genetic Approaches to Neural Circuits in the Mouse
Josh Huang, Z., & Zeng, H. (2013) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-062012-170307 · Cited by 200
Magnetoelectric materials for miniature, wireless neural stimulation at therapeutic frequencies
Singer, A., Dutta, S., Lewis, E., Chen, Z., Chen, J. C., Verma, N., Avants, B., Feldman, A. K., O’Malley, J., Beierlein, M., et al. (2020) · Neuron · doi:10.1016/j.neuron.2020.05.019 · Cited by 202
Metadata
- Authors
- Jin, L., Han, Z., Platisa, J., Wooltorton, J. R. A., Cohen, L. B., & Pieribone, V. A.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 75
- Issue
- 5
- Pages
- 779-785
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2069950623
- Cited by
- 539
- References
- 0
- FWCI
- 17.85
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. https://app.cochat.ai/papers/10.1016/j.neuron.2012.06.040
- The paper
- Jin, L., Han, Z., Platisa, J., Wooltorton, J. R. A., Cohen, L. B., & Pieribone, V. A. (2012). Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron. https://doi.org/10.1016/j.neuron.2012.06.040
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
