Chemoconnectomics: Mapping Chemical Transmission in Drosophila
Open accessAbstract
We define the chemoconnectome (CCT) as the entire set of neurotransmitters, neuromodulators, neuropeptides, and their receptors underlying chemotransmission in an animal. We have generated knockout lines of Drosophila CCT genes for functional investigations and knockin lines containing Gal4 and other tools for examining gene expression and manipulating neuronal activities, with a versatile platform allowing genetic intersections and logic gates. CCT reveals the coexistence of specific transmitters but mutual exclusion of the major inhibitory and excitatory transmitters in the same neurons. One neuropeptide and five receptors were detected in glia, with octopamine β2 receptor functioning in glia. A pilot screen implicated 41 genes in sleep regulation, with the dopamine receptor Dop2R functioning in neurons expressing the peptides Dilp2 and SIFa. Thus, CCT is a novel concept, chemoconnectomics a new approach, and CCT tool lines a powerful resource for systematic investigations of chemical-transmission-mediated neural signaling circuits underlying behavior and cognition.
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The usage of acetylcholine, glutamate, and GABA is largely mutually exclusive.
“the usage of acetylcholine, glutamate, and GABA is largely mutually exclusive.30,31”
From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [30]
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- Authors
- Deng, B., Li, Q., Liu, X., Cao, Y., Li, B., Qian, Y., Xu, R., Mao, R., Zhou, E., Zhang, W., Huang, J., & Rao, Y.
- Affiliations
- Peking University (CN)
- Nanjing Medical University (CN)
- Corresponding author
- Rao, Yi
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2019-03
- Volume
- 101
- Issue
- 5
- Pages
- 876-893.e4
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2916714824
- Cited by
- 340
- References
- 6
- Citations by year
- 2026: 40 · 2025: 72 · 2024: 57 · 2023: 66 · 2022: 42 · 2021: 29 · 2020: 23 · 2019: 11
- FWCI
- 12.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Beijing Commission of Science and Technology (Z151100003915121)
- Beijing Advanced Innovation Center for Genomics
- National Natural Science Foundation of China (31421003)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- neurotransmitters, neuromodulators, neuropeptides, neurotransmitter receptors, Drosophila, knockout lines, sleep regulation, DILP2, SifA
- MeSH terms
- Animals, Drosophila melanogaster, Synaptic Transmission, Neuroglia, Neurons, Neurotransmitter Agents, Connectome
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