Expression of Ski and its role in astrocyte proliferation and migration
Abstract
Ski, as an evolutionarily conserved protein, is a versatile transcriptional regulator which widely distributes in various tissues and species. Recently, we have demonstrated for the first time that Ski was strikingly up-regulated in reactive astrocytes after spinal cord injury (SCI) in vivo, which indicates that maybe Ski is a new molecule that controls astrocytes' biological properties after SCI. However, the accurate distributions and functions of Ski in astrocytes after central nervous system (CNS) injury are still unclear. Astrocytes were collected from rats' cerebral cortex. To elucidate the expression and role of Ski in reactive astrocytes, we performed an activated astrocytes model induced by LPS and scratch injury in vitro. Our results showed that Ski gradually increased and reached a peak at 4days, then declined at 6days after induction by LPS. Up-regulation of Ski was accompanied with the increase in proliferation-related proteins including PCNA, CDK4 and CyclinD1. Furthermore, immunofluorescent staining analysis also demonstrated a highly positive relationship between Ski and GFAP, PCNA in astrocytes. These results indicated that Ski might play an important role in astrocyte proliferation. To further explore the role of Ski, astrocytes were transfected with Ski-specific small interfering RNA (siRNA). We found that the primary activated astrocytes' proliferation decreased significantly after transfection with Ski-specific siRNA. Surprisingly, Ski knockdown also weak…
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PI3K-Akt signaling can regulate actin activity and is important in astrocyte proliferation and migration.
“It has been suggested that PI3K-Akt signaling can regulate actin activity and is important in astrocyte proliferation and migration (Zhao et al., 2017;Liao et al., 2022;Pérez-Núñez et al., 2023).”
From Cell Adhesion Molecule Protocadherin-γC5 Ameliorates Aβ Plaque Pathogenesis by Modulating Astrocyte Function in Alzheimer's Disease (2025) · cites this paper as Zhao et al., 2017
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Middeldorp, J., & Hol, E. M. (2011) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2011.01.005 · Cited by 1,080 · see citing works
Sofroniew, M. V. (2015) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a020420 · Cited by 695 · see citing works
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Sun, X., Pan, S., Li, D., Su, M., Zheng, H., Zhang, Y.-W., & Li, Y. (2025) · The Journal of Neuroscience · doi:10.1523/jneurosci.0967-24.2025 · Cited by 4 · see citing works
- Authors
- Zhao, X., Wang, X.-W., Zhou, K.-S., Nan, W., Guo, Y.-Q., Kou, J.-L., Wang, J., Xia, Y.-Y., & Zhang, H.-H.
- Affiliations
- Lanzhou University Second Hospital (CN)
- Corresponding author
- Zhang, H.-H.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 362
- Pages
- 1-12
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2749517334
- Cited by
- 22
- References
- 2
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 1
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Lanzhou University Institutional Repository (submittedVersion)
- Funders
- National Natural Science Foundation of China (30772299, 81672207)
- Second Hospital of Lanzhou University
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Spinal Cord Injury Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- reactive astrocytes, astrocyte proliferation, astrocyte migration, spinal cord injury, PCNA, CDK4, cyclin D1, glial fibrillary acidic protein, transcriptional regulation, central nervous system injury, siRNA knockdown
- MeSH terms
- Animals, Astrocytes, Cell Cycle, Cell Movement, Cells, Cultured, Cerebral Cortex, Glial Fibrillary Acidic Protein, Lipopolysaccharides, Proto-Oncogene Proteins, Wound Healing, Rats, Sprague-Dawley, Proliferating Cell Nuclear Antigen, Cyclin D1, RNA, Small Interfering, Cell Proliferation, Cyclin-Dependent Kinase 4
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