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Cited by 20Photodynamic Therapy Research StudiesNonmelanoma Skin Cancer StudiesCervical Cancer and HPV Research

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Induction of apoptosis in HPV16 E7 transfected human keratinocyte by ALA-mediated photodynamic therapy

Miao, Fei, Lv, Ting, Zhang, Yunfeng, Huang, Zheng, Wang, Xiuli, Wang, Hongwei·2016·Photodiagnosis and Photodynamic Therapy
DOI 10.1016/j.pdpdt.2015.07.004
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Abstract

Background and objective 5-Aminolevulinic acid (ALA)-mediated photodynamic therapy (ALA-PDT) is effective for the treatment of condyloma and intraepithelial neoplasia associated with human papillomaviruses (HPV). However, the mechanism of PDT on HPV-infected host cells is not fully understood. The aim of this in vitro study was to examine ALA-PDT induced apoptosis in a high-risk HPV E7 gene transfected cell line. Materials and methods HPV16 E7 gene obtained from HPV16 positive human cervical carcinoma was transfected into a human keratinocyte cell line. PpIX generation and ALA-PDT-induced apoptosis and mitochondrial damage were investigated in vitro. Results Fluorescence examination showed that HPV16E7 transfected cells could produce PpIX fairly quickly in the presence of 1mM ALA. Apoptosis occurred after ALA-PDT at dose levels of 4, 8, and 12 J/cm(2). Mitochondrial potential collapse occurred as early as 2 h post PDT. ALA-PDT induced a relatively weak and shorter caspase-9 activation but a strong and long lasting caspase-3 activation. However, mRNA levels of caspase-9 and -3 genes in cells treated with ALA-PDT were unchanged. Conclusion This in vitro study demonstrated that HPV16 E7 transfected keratinocytes could generate PpIX in the presence of ALA. Under light irradiation, ALA-PDT could induce mitochondrial-dependent apoptosis in HPV16 E7 transfected cells.

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  • Loss of mitochondrial membrane potential induces apoptotic cell death by activating the caspase-9-mediated mitochondrial apoptotic pathway.

    “Consequently, the loss of mitochondrial membrane potential induces apoptotic cell death by activating the caspase-9-mediated mitochondrial apoptotic pathway, as shown in multiple studies with various cell lines [25,26,27,28].”

    From 5-Aminolevulinic Acid as a Theranostic Agent for Tumor Fluorescence Imaging and Photodynamic Therapy (2023) · cites this paper as [27]

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Metadata

Authors
Miao, F., Lv, T., Zhang, Y., Huang, Z., Wang, X., & Wang, H.
Affiliations
  • Fudan University (CN)
  • Shanghai Skin Disease Hospital (CN)
  • Fujian Normal University (CN)
Corresponding author
Wang, Hongwei
ORCID
Venue
Photodiagnosis and Photodynamic Therapy
Publisher
Elsevier BV
Published
2016-03
Volume
13
Pages
205-210
ISSN
1572-1000 (ISSN-L)
DOI
10.1016/j.pdpdt.2015.07.004
OpenAlex
W962310441
Cited by
20
References
40
Citations by year
2026: 2 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2
FWCI
0.26
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Photodiagnosis and Photodynamic Therapy (publishedVersion)
  • PubMed (publishedVersion)
Funders
Type A Major Project of Shanghai Health Bureau (20124034)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Photodynamic Therapy Research Studies
  • Health Sciences › Medicine › Dermatology › Nonmelanoma Skin Cancer Studies
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
Keywords
5-aminolevulinic acid, photodynamic therapy, HPV16 E7, human papillomavirus, apoptosis, keratinocytes, protoporphyrin IX, mitochondrial depolarization, caspase-3, caspase-9, mitochondrial-dependent apoptosis
MeSH terms
Aminolevulinic Acid, Cell Line, Humans, Light, Photochemotherapy, Protoporphyrins, Transfection, Keratinocytes, Treatment Outcome, Apoptosis, Photosensitizing Agents, Papillomavirus E7 Proteins

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