Reflections on more than 10 years of TGE approaches
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When the need for a quick and economical approach prevails, transient expression remains the best choice for protein production.
“When the need for a quick and economical approach prevails, transient expression remains the best choice for protein production (Rosser et al., 2005;Pham et al., 2006;Geisse, 2009;Chahal et al., 2011;Baldi et al., 2012).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Geisse, 2009
Cites
Large-Scale transfection of mammalian cells for the fast production of recombinant protein
Pham, P. L., Kamen, A., & Durocher, Y. (2006) · Molecular Biotechnology · doi:10.1385/mb:34:2:225 · Cited by 236 · see citing works
Jiang, Z., & Sharfstein, S. T. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21726 · Cited by 118 · see citing works
Ohya, T., Hayashi, T., Kiyama, E., Nishii, H., Miki, H., Kobayashi, K., Honda, K., Omasa, T., & Ohtake, H. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21758 · Cited by 96 · see citing works
Rosser, M. P., Xia, W., Hartsell, S., McCaman, M., Zhu, Y., Wang, S., Harvey, S., Bringmann, P., & Cobb, R. R. (2005) · Protein Expression and Purification · doi:10.1016/j.pep.2004.07.015 · Cited by 68 · see citing works
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Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Geisse
- Venue
- Protein Expression and Purification
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 64
- Issue
- 2
- Pages
- 99-107
- ISSN
- 1046-5928 (ISSN-L)
- OpenAlex
- W2045408545
- Cited by
- 117
- References
- 4
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 1 · 2020: 7 · 2019: 3 · 2018: 8 · 2017: 10 · 2016: 3 · 2015: 9 · 2014: 10 · 2013: 13 · 2012: 16
- FWCI
- 0.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Protein Expression and Purification (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- cell density effects, transient expression, high cell density, cell-specific productivity, recombinant protein expression, bioprocess scale-up, cellular metabolism, biopharmaceutical manufacturing
- MeSH terms
- Animals, Cells, Cultured, Cricetulus, Cricetinae, Humans, Transfection, Gene Expression, CHO Cells
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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