Anti-melanogenesis effect of dehydroglyasperin C through the downregulation of MITF via the reduction of intracellular cAMP and acceleration of ERK activation in B16F1 melanoma cells
Abstract
Background In mammals, UV radiation induces melanin synthesis in melanocyte for protecting their skin through the stimulation of α-melanocyte stimulating hormone (α-MSH) from keratinocytes. In this study, the inhibitory effects of dehydroglyasperin C (DGC), an useful component of Glycyrrhiza uralensis (G. uralensis), was investigated on melanogenesis induced by α-melanocyte stimulating hormone (α-MSH) and its mechanisms. Methods Melanogenesis suppression effect of DGC on α-MSH induced B16F1 melanoma cells. The cell viability was measured by MTT assay. Expression and phosphorylation of melanogeic protein were conducted using western blot. cAMP acceleration was measured by cAMP immunoassay kit. To investigate whitening mechanism, we used ERK inhibitor (PD98059). Results DGC decreased intra cellular tyrosinase (TYR) activity and expression of melanin synthesis related proteins (TYR and TRP-1) in a dose-dependent manner on α-MSH induced melanogenesis. In addition, DGC induced the downregulation of MITF (melanocyte-specific transcription factor) through suppression of cAMP-CREB pathway. Also, phosphorylation of extracellular signal regulated kinase (ERK) decreased MITF by DGC treatment. Conclusion Therefore, DGC could be used as a whitening ingredient in skin and clinical usage against hyperpigmentation.
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Dehydroglyasperin C dose-dependently decreased intracellular tyrosinase activity and expression of TYR and TRP-1 in keratinocytes treated with α-MSH to induce melanogenesis.
“Dehydroglyasperin C decreased in a dose-dependent manner intracellular tyrosinase activity and expression of proteins related to melanin synthesis (TYR and TRP-1) in keratinocytes treated with α-MSH (melanocyte stimulating hormone) to induce melanogenesis [46].”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [46]
Dehydroglyasperin C treatment decreased MITF via phosphorylation of extracellular signal-regulated kinase (ERK).
“Phosphorylation of extracellular signal-regulated kinase (ERK) also decreased MITF by dehydroglyasperin C treatment [46].”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [46]
Dehydroglyasperin C reduced the downregulation of MITF through suppression of the cAMP-CREB pathway.
“Dehydroglyasperin C also reduced the downregulation of MITF (melanocyte-specific transcription factor) through suppression of cAMP-CREB pathway.”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [46]
Dehydroglyasperin C could be considered a whitening ingredient against hyperpigmentation in the skin.
“Literature reports how dehydroglyasperin C could be considered a whitening ingredient against hyperpigmentation in the skin.”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [46]
Cites
Ando, H., Niki, Y., Ito, M., Akiyama, K., Matsui, M. S., Yarosh, D. B., & Ichihashi, M. (2012) · Journal of Investigative Dermatology · doi:10.1038/jid.2011.413 · Cited by 258 · see citing works
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Cerulli, A., Masullo, M., Montoro, P., & Piacente, S. (2022) · Cosmetics · doi:10.3390/cosmetics9010007 · Cited by 83 · see citing works
- Authors
- Lim, J. W., Ha, J. H., Jeong, Y. J., & Park, S. N.
- Affiliations
- Seoul National University of Science and Technology (KR)
- Corresponding author
- Park, Soo Nam
- Venue
- Pharmacological Reports
- Published
- 2018-10
- Volume
- 70
- Issue
- 5
- Pages
- 930-935
- ISSN
- 1734-1140 (ISSN-L)
- OpenAlex
- W2794358746
- Cited by
- 25
- References
- 1
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 6 · 2020: 4 · 2019: 2
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pharmacological Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Trade, Industry and Energy, Republic of Korea (RDNWIC15013)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
- Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
- Keywords
- Glycyrrhiza uralensis, anti-melanogenesis, melanogenesis, MITF, ERK1/2 phosphorylation, tyrosinase, TRP-1, alpha-melanocyte-stimulating hormone, B16F1 melanoma cells, hyperpigmentation, skin lightening
- MeSH terms
- Cyclic AMP, alpha-MSH, Animals, Benzopyrans, Cell Survival, Dose-Response Relationship, Drug, Enzyme Activation, Flavonoids, Melanins, Melanoma, Experimental, Membrane Glycoproteins, Oxidoreductases, Monophenol Monooxygenase, Down-Regulation, Cell Line, Tumor, Extracellular Signal-Regulated MAP Kinases, Mice, Microphthalmia-Associated Transcription Factor
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