Evaluation of the neuroprotective activity of P. amarus in attenuating arsenic-induced neurotoxicity - an in vivo study
Open accessAbstract
Arsenic is viewed as a bizarre and frightful element in human history. Recent studies showed that even low levels of arsenic can cause neurological damage. Phyllanthus amarus is an important medicinal herb proved to have neuro protective effect in experimental animals. This pre-clinical study used an animal model of arsenic toxicity and screened the different neuroprotective mechanisms of Phyllanthus amarus ethanolic extract. To assess the effect of Phyllanthus amarus ethanolic extract on various biochemical parameter's in the brain of arsenic administered Wistar albino rats. The neuroprotective activity of P. amarus against arsenic induced toxicity was studied in Wistar albino rats. The experimental animals (n=24) were divided into four groups of 6 rats each. Rats in group I received distilled water, whereas rats in groups II, III, and IV received arsenic as sodium arsenite salt (40 mg/kg b.w) in drinking water daily for 28 days. In addition, rats from groups III, and IV also received P. amarus ethanolic extract 100 and 200 mg/kg b.w, by intragastric route, once daily for a period of 28 days. At the end of the experiment rats were subjected to various neurobehavioral tests such as Elevated plus maze, Light dark arena, Forced swim test and Tail suspension test. After 24 h of the last treatment, the animals were euthanized and brain tissue was assayed for various biochemical parameters. ANOVA and Post hoc testing of behavioral and biochemical data (Dunnett's t-test and Student unpaired T test) showed co-administration of P. amarus ethanolic extract significantly reversed anxiety and depression-like behaviors in experimental animals. P. amarus ethanolic extract effectively reduced acetyl cholinesterase activity in the brain, and it also increased the levels of dopamine and serotonin in the brains of arsenic-treated animals as compared to only arsenic treated group. P. amarus ethanolic extract significantly decreased lipid peroxidase and increased brain catalase and super oxidase dismutase activities. Based on the pharmacological, and biochemical analysis the plant extracts of P. amarus have shown protective effects against arsenic induced neurotoxicity.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Phyllanthus acidus ethanolic extract increases super oxidase dismutase and brain catalase activities.
“increased super oxidase”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [158]
Phyllanthus acidus has AChE inhibitory potential.
“AChE”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [158]
Phyllanthus acidus ethanolic extract significantly decreases lipid peroxidase.
“significantly decreased”
From Unlocking the Therapeutic Potential of Medicinal Plants for Alzheimer’s Disease: Preclinical to Clinical Trial Insights (2023) · cites this paper as [158]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 28 · Cited by 11
Research works printed but not linked: 4
- Mao, X., Wu, L.F., Guo, H.L., Chen, W.J., Cui, Y.P., Qi, Q., Li, S., 2016. The genus Phyllanthus: An ethnopharmacological, phytochemical, and pharmacological review. Evid. Based Complement. Altern. 1-36.
- Massoulie, J., Sussman, J., Bon, S., Silman, I., 1993. Structure and functions of acetylcholinesterase and butyrylcholinesterase. Progress in Brain Research, Chapter 15, 98. Elsevier Science Publishers B.V, pp. 139-146.
- Refaey, H.E., Amri, H.S., 2011. Effects of Antidepressants on Behavioural Assessment in Adolescent Rats. Bahrain Med Bull 33 (2), 1-12.
- Rahman, H., Eswaraiah, M.C. 2008. Simple Spectroscopic Methods for Estimating Brain Neurotransmitters, Antioxidant Enzymes of Laboratory Animals Like Mice: A Review, Available from: https://www.pharmatutor.org/articles/simple-spectroscop ic-method-estimating-brain-neurotransmitter-antioxidnat-enzymes-lab-animals. [Last accessed on 2021 March 28].
- Authors
- Hashim, A., Ahmed, M. G., Rahiman, N. B. A., Manikkoth, S., & Leena Pramod, K.
- Affiliations
- Yenepoya University (IN)
- Corresponding author
- Hashim, A.
- Venue
- Phytomedicine Plus
- Publisher
- Elsevier BV
- Published
- 2022-08
- Volume
- 2
- Issue
- 3
- Pages
- 100316
- ISSN
- 2667-0313 (ISSN-L)
- OpenAlex
- W4283732396
- Cited by
- 11
- References
- 28
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 4
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Phytomedicine Plus (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Phytochemistry and Bioactivity Studies
- Keywords
- Phyllanthus amarus, arsenic neurotoxicity, neuroprotection, sodium arsenite, ethanolic plant extract, anxiety-like behavior, depression-like behavior, acetylcholinesterase, dopamine, serotonin, oxidative stress, antioxidant enzymes, lipid peroxidation, neurochemistry
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
