Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism
Open accessAbstract
Hepatic steatosis is a highly prevalent liver disease, yet research on it is hampered by the lack of tractable cellular models in poultry. To examine the possibility of using organoids to model steatosis and detect it efficiently in leghorn male hepatocellular ( LMH ) cells, we first established steatosis using different concentrations of oleic acid ( OA ) (0.05–0.75 mmol/L) for 12 or 24 h. The subsequent detections found that the treatment of LMH cells with OA resulted in a dramatic increase in intracellular triglyceride ( TG ) concentrations, which was positively associated with the concentration of the inducing OA (R 2 > 0.9). Then, the modeled steatosis was detected by flow cytometry after NileRed staining and it was found that the intensity of NileRed-A was positively correlated with the TG concentration (R 2 > 0.93), which demonstrates that the flow cytometry is suitable for the detection of steatosis in LMH cells. According to the detection results of the different steatosis models, we confirmed that the optimal induction condition for the establishment of the steatosis model in LMH cells is OA (0.375 mmol/L) incubation for 12 h. Finally, the transcription and protein content of fat metabolism-related genes in steatosis model cells were detected. It was found that OA-induced steatosis could significantly decrease the expression of nuclear receptor PPAR-γ and the synthesis of fatty acids (SREBP-1C, ACC1, FASN), increasing the oxidative decomposition of triglycerides (CPT1A) and the assembly of low-density lipoproteins (MTTP, ApoB). Sterol metabolism in model cells was also significantly enhanced (HMGR, ABCA1 , L-BABP). This study established, detected, and analyzed an OA-induced steatosis model in LMH cells, which provides a stable model and detection method for the study of poultry steatosis-related diseases.
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Oleic acid primarily promotes the formation of larger lipid droplets and facilitates the safe esterification of fatty acids into triglycerides, whereas palmitic acid is more strongly associated with mitochondrial fragmentation, increased oxidative metabolism, and ROS formation in both the cytosol and mitochondria.
“Oleic acid primarily promotes the formation of larger lipid droplets and facilitates the safe esterification of fatty acids into triglycerides [148,149,150,151], whereas palmitic acid is more strongly associated with mitochondrial fragmentation, increased oxidative metabolism, and ROS formation in both the cytosol and mitochondria.”
From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [151]
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- Authors
- Song, H., Yang, R., Zhang, J., Sun, P., Xing, X., Wang, L., Sairijima, T., Hu, Y., Liu, Y., Cheng, H., Zhang, Q., & Li, L.
- Affiliations
- Xinjiang Production and Construction Corps (CN)
- Corresponding author
- Li, Lianrui
- Venue
- Poultry Science
- Publisher
- Elsevier BV
- Published
- 2023-01
- Volume
- 102
- Issue
- 1
- Pages
- 102297
- ISSN
- 0032-5791 (ISSN-L)
- PMC
- PMC9709224
- OpenAlex
- W4307815352
- Cited by
- 19
- References
- 23
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 7 · 2023: 1
- FWCI
- 2.71
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Poultry Science (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- hepatic steatosis, oleic acid, LMH cells, triglyceride accumulation, PPARγ, SREBP-1c, fatty acid synthesis, triglyceride oxidation, sterol metabolism, lipid metabolism, organoid models
- MeSH terms
- Animals, Chickens, Fatty Acids, Fatty Liver, Liver, Male, Oleic Acid, Lipid Metabolism
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