Cited by 403Biofuel production and bioconversionAnaerobic Digestion and Biogas ProductionThermochemical Biomass Conversion Processes

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Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse

Binod, Parameswaran, Satyanagalakshmi, Karri, Sindhu, Raveendran, Janu, Kanakambaran Usha, Sukumaran, Rajeev K., Pandey, Ashok·2012·Renewable Energy

DOI 10.1016/j.renene.2011.06.007

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“The absorption peak at 1000–1200 cm−1 results from C–O single-bond vibration, possibly reflecting the structural and characteristic peak of cellulose and hemicellulose, the glycosidic bond, or the ether bond between lignin and hemicellulose [44].”
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“Similarly, in sugarcane bagasse, the peak at 2890.49 cm −1 of untreated and 2876.82 cm −1 of pretreated shows the weak C-H stretching [29].”
Influential
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“In addition, the broad peaks at 3000– 2900 cm for lignin, characterized by the aromatic ring [40], C–H and O–H stretching [36] were also present in samples treated with P.”
Background
Advances in Biochemical Engineering Biotechnology
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“Major advantages of microwave irradiation are uniform heating, higher rate, efficiency, selectivity (delignification), hotspot formation, precision and controlled heating (Binod et al. 2012).”
Background
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“Other workers (Binod et al., 2012; Chen et al., 2011) found using acid or alkali in combination with”
BackgroundInfluential
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A study on glycerolysis of oil palm empty fruit bunch fiber.
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“Similar structural changes were reported earlier for sugarcane bagasse pretreated with short duration microwave (34) and rice straw pretreated with ammonia water soaking pretreatment (35).”
Background
Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood
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“The absorption peaks at wavenumbers 3300 cm−1 (A) and 2895 cm−1 (B) corresponded to the O-H hydrogen bond of cellulose and C-H stretching vibration of cellulose and lignin, respectively (Binod et al. 2012).”
Background
The effect of microwave power on lignocellulose content, physical and chemical characteristics of biomass: A review
Indriani, D. W., Susilo, B., & Mashur (2021) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/924/1/012069 · Cited by 11
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“Though there are many literatures available for the microwave assisted pre-treatment of the biomass and extraction of hemicelluloses from biomass using high temperature and/or pressure [13,23-29], only very few literatures are available on microwave assisted extraction of hemicelluloses under mild conditions [30,31].”
Background
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“Both these peaks were weaker in the spectrum of HRS, indicating the removal of the linkages between hemicellulose and lignin, which provided additional evidence for the successful conversion of hemicelluloses [49].”
Background
Enhancing fermentable sugar yield from cassava pulp for bioethanol production: microwave-coupled enzymatic hydrolysis approach
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“Binod et al. ( 2012 ) developed a process using biomass microwave treatment in alkali solution (1 % NaOH) followed by acid pretreatment (1 % H 2 SO 4 ) and enzymatic hydrolysis, achieving an overall reduction of sugar yield of 0.83 g/g dry sugarcane bagasse.”
MethodInfluential
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“Besides hemicellulose and lignin removal, vibrational changes in the biomass were observed at wavenumbers 1037 cm –1 and 2892 cm –1 , indicating the nature of lime to alter the structure of cellulose and lignin [61].”
Background
Photocatalytic oxidation of textile dye using sugarcane bagasse-Nb2O5 as a catalyst
de Almeida, A. R., Monteiro, F. C., Monteiro, J. F. H. L., Tiburtius, E. R. L., & Pessôa, C. A. (2022) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/j.jphotochem.2022.114103 · Cited by 9
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Optimization of dilute sulfuric acid pretreatment of kitchen garbage for increased lactic acid production
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“C of cellulose, MA anti-symmetrical bridge oxygen [56].”
Effects of washing with water on enzymatic saccharification and d-lactic acid production from steam-exploded sugarcane bagasse
Sasaki, C., Okumura, R., Asakawa, A., Asada, C., & Nakamura, Y. (2012) · Journal of Material Cycles and Waste Management · doi:10.1007/s10163-012-0064-y · Cited by 9
A review of agro-food waste transformation into feedstock for use in fermentation.
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Introduction
Binod, P., & Pandey, A. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800080-9.00001-3 · Cited by 9
Self-citation
Xylooligosaccharides from Barley Malt Residue Produced by Microwave-Assisted Enzymatic Hydrolysis and Their Potential Uses as Prebiotics
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“This pretreatment increased enzymatic hydrolysis through lignin removal selectively Binod et al. [57] reported that microwave pretreatment was enough to obtain high degradation of the cell wall, which increased the surface area of BMR towards enzyme contact.”
Highly Efficient Base-Catalyzed Valorization of Food Waste into Lactic Acid at Mild Conditions with the Pretreatment Using HCl Recovered from PVC
Yang, R., Chen, P., Zhong, H., & Jin, F. (2023) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.3c04957 · Cited by 8
Biodegradation of Cellulose and Agricultural Waste Material
Akhtar, N., Goyal, D., & Goyal, A. (2015) · CRC Press eBooks · doi:10.1201/b18218-12 · Cited by 8
Physicochemical treatment for improving bioconversion of cassava industrial residues
Sudha, A., Sivakumar, V., Sangeetha, V., & Devi, K. (2017) · Environmental Progress & Sustainable Energy · doi:10.1002/ep.12702 · Cited by 8
“The lignin in the acid pretreated solid residue lowers the enzymatic convertibility due to its inhibitory action [14].”
Background
Evaluation of Blend Production of Cellulases and Xylanases Using Pretreated and Recycled Carnauba Straw
da Silva, F. L., dos Santos, D. A., Campos, A. D. O., Magalhães, E. R. B., & dos Santos, E. S. (2021) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-021-03677-8 · Cited by 8
Microwave-Assisted Alkali Delignification Coupled with Non-Ionic Surfactant Effect on the Fermentable Sugar Yield from Agricultural Residues of Cassava
S, P. N., & Padmaja, G. (2017) · International Journal of Environment Agriculture and Biotechnology · doi:10.22161/ijeab/2.2.10 · Cited by 7
“Microwaves interact with both polar molecules and ions in LCBs and produce thermal and non-thermal effects, which help breakdown the biomass in a shorter time compared to conventional heating [19, 20].”
Background
Innovative Valorization of Wood Panel Waste into Activated Biochar for Efficient Phenol Adsorption
Bentis, A., Ceron Daza, L. D., Dia, M., Koubaa, A., & Braghiroli, F. L. (2025) · Applied Sciences · doi:10.3390/app15158518 · Cited by 7
Influence of Microwave-Assisted Chemical Thermohydrolysis of Lignocellulosic Waste Biomass on Anaerobic Digestion Efficiency
Dębowski, M., Zieliński, M., Nowicka, A., & Kazimierowicz, J. (2024) · Energies · doi:10.3390/en17174207 · Cited by 7
Microwave-assisted alkaline pretreatment of white grape pomace for the production of bioethanol
Han, Y., Shan, C., Zeng, H., Shi, S., & Han, L. (2025) · RSC Advances · doi:10.1039/d5ra04296h · Cited by 7
Investigation of Microwave Irradiation and Ethanol Pre-Treatment toward Bioproducts Fractionation from Oil Palm Empty Fruit Bunch
Gill, A. S., Wong, K. H., Lim, S., Pang, Y. L., Ling, L., & Lau, S. Y. (2024) · Sustainability · doi:10.3390/su16031275 · Cited by 8
“…degrade the OPEFB, helping the ethanol solvent to be more active in breaking the lignin protective layer of the biomass. pointed out that microwave-assisted extraction methods, as opposed to microwave irradiation alone, can lead to a significant yield of fermentable sugars and lignin removal [21].”
Background
Harnessing fermentation for sustainable beverage production: A tool for improving the nutritional quality of coffee bean and valorizing coffee byproducts
Aloo, S. O., Gemechu, F. G., Oh, H.-J., Kilel, E. C., Chelliah, R., Gonfa, G. H., & Oh, D. (2024) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2024.103263 · Cited by 7
Microwave and microwave-alkali effect on barley straw for total sugar yield
İnan, H., Turkay, O., & Akkiris, C. (2014) · International Journal of Global Warming · doi:10.1504/ijgw.2014.061011 · Cited by 7
“Pretreatment is an important way of practical cellulose conversion processes and pretreatment can take place before enzymatic hydrolysis effectively (Binod et al., 2012).”
BackgroundMethodResultInfluential
ENZYMATIC SACCHARIFICATION ON AMMONIA PRE-TREATED OIL PALM TRUNK BIOMASS FOR GLUCOSE PRODUCTION: AN OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY
Lai, L. W., Yahya, S. S. M., Norakma, M. N., & Sulong, M. R. (2016) · Malaysian Journal of Analytical Science · doi:10.17576/mjas-2016-2001-03 · Cited by 7
A solid-state microwave method to disrupt biomass microstructure for natural product extraction
See, T. Y., Yusoff, R. B., Chan, C., & Ngoh, G. C. (2018) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2018.03.002 · Cited by 7
Effect Of Pre-Treatment And Inoculant During Composting Of Palm Oil Empty Fruit Bunches
Yaser, A. Z., Yee, I. K. T., Thian, E. S. C., Heng, S. Y., Janaun, J., Chong, K. P., Haywood, S. K., Tan, V. D., Asis, T., & Al-Mizi, T. M. T. M. A. (2018) · ASEAN Journal of Chemical Engineering · doi:10.22146/ajche.49551 · Cited by 7
“Microwave pretreatment was carried out in a domestic microwave (Model: ELBA, EMO-A2072(SV)) at a power of approximately 487 W for an exposure time of 4 minutes (Binod et al., 2012).”
BackgroundMethodInfluential
Evaluation Solvent Level Effect on Sugar Yield during Microwave-Assisted Pretreatment
Ethaib, S., Omar, R., Mazlina, M. K. S., Biak, D. R. A., & Zuwaini, M. (2020) · IOP Conference Series Materials Science and Engineering · doi:10.1088/1757-899x/871/1/012034 · Cited by 7
Two-stage alkaline and acid pretreatment applied to sugarcane bagasse to enrich the cellulosic fraction and improve enzymatic digestibility
Igbojionu, L. I., Laluce, C., & Pécoraro, É. (2020) · Detritus · doi:10.31025/2611-4135/2020.14005 · Cited by 6
“The sample was refluxed in water for 30 min to remove the remaining solvents before being dried in a Biochemical Oxygen Demand (BOD) incubator, as recommended in the literature (Binod et al., 2012) at 60°C to constant weight before being stored in a desiccator at room temperature until use.”
Method
Potential brewer’s spent grain as a carbon source alternative for biosurfactant production by Rhodotorula mucilaginosa (LBP4)
Azevedo, M. A., Neta, M. D. R. A. V., do Nascimento, L. P., da Silva, G. F., Vicente, J. G. P., & Duarte, I. C. S. (2023) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2023.111594 · Cited by 6
Fermentative lactic acid production from Sotol bagasse (Dasylirion spp.): Optimization of microwave-assisted biomass pre-treatment
Orozco-Mena, R. E., Lorena, T. A. N., Salmerón, I., Piñón-Muñiz, M. I., Espinoza-Hicks, J. C., Chávez‐Flores, D., Bacayo, J., & Ramos-Sánchez, V. H. (2025) · Waste Management · doi:10.1016/j.wasman.2025.114778 · Cited by 6
Microwave and microwave-chemical pretreatment application for agricultural waste
İnan, H., Turkay, O., & Akkiris, C. (2015) · Desalination and Water Treatment · doi:10.1080/19443994.2015.1069217 · Cited by 6
Novel Pretreatment Methods to Improve Enzymatic Saccharification of Sugarcane Bagasse: A Report
Saeed, S., & Saleem, M. (2018) · DOAJ (DOAJ: Directory of Open Access Journals) · Cited by 6
Removal of Cr(VI) from water by in natura and magnetic nanomodified hydroponic lettuce roots
Soares, B. C., Abilio, T. E., José, J. C., Labuto, G., & Carrilho, E. N. V. M. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-022-21755-0 · Cited by 6
Comparative pretreatment method for efficient enzymatic hydrolysis of Salvinia cucullata and sewage treatment in ponds containing this biomass
Mishra, G. S., Mitra, A., Banerjee‬‬‬‬‬‬‬‬‬, R., & Ghangrekar, M. M. (2013) · Clean Technologies and Environmental Policy · doi:10.1007/s10098-013-0694-3 · Cited by 6
“The enhancement of absorption peak of –CH2 near 2,900 cm -1 is the distinguished feature of cellulose (Binod et al. 2012).”
Background
Efficient Hydrolysis of Lignocellulosic Biomass: Potential Challenges and Future Perspectives for Biorefineries
Mukherjee, G., Dhiman, G., & Akhtar, N. (2017) · Environmental science and engineering · doi:10.1007/978-3-319-48439-6_17 · Cited by 7
Bioconversion of lignocellulose in ensiled Caragana korshinskii Kom. into bioethanol by ferulic acid esterase-producing Limosilactobacillus reuteri A4-2 and Acremonium cellulase
Zhang, Y., Kreuzer, M., Usman, S., Liang, Y., Su, R., Li, Q., Xu, D., Yu, P., & Guo, X. (2025) · Industrial Crops and Products · doi:10.1016/j.indcrop.2025.121384 · Cited by 5
Removal of Steryl Glucosides in Biodiesel Using Diatomaceous Earth as Adsorbent
Yuangsawad, R., Khambung, S., Laungthaleongpong, P., & Na‐Ranong, D. (2017) · doi:10.18690/978-961-286-048-6.12 · Cited by 4
Exploring breadfruit peel waste as a potential source for bio-ethanol production
Chithra, M., Krishna, K. R., Rakesh, S., Kumar, A. R., & Manoprabha, M. (2024) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-024-06043-w · Cited by 5
“H stretching [41].”
"MICROWAVE-ASSISTED ALKALI PRETREATMENT OF HAPLOPHRAGMA ADENOPHYLLUM LEAVES FOR BIOETHANOL PRODUCTION"
Rubab, N., Ghazanfar, M., ADNAN, S., Ahmad, I., Shakir, H. A., Khan, M. I., Franco, M., & Irfan, M. I. (2023) · Cellulose Chemistry and Technology · doi:10.35812/cellulosechemtechnol.2023.57.30 · Cited by 5
Nonindustrial pretreatment and enzymes can yield sufficient calories from lignocellulosic biomass for human survival
Siva, N., & Anderson, C. T. (2024) · Food Science & Nutrition · doi:10.1002/fsn3.4358 · Cited by 4
“However, common thermal treatments involve a temperature range of 140–230°C (Baharuddin et al., 2012; Binod et al., 2012; Lee et al., 2010; Zhang et al., 2022), which could not be easily achieved at a household level using pots or pressure cookers that typically operate at 100–120°C.”
Background
Synthesis of Microcrystalline Cellulose at Several Variations in Alpha-Cellulose Hydrolysis Time from Oil Palm Empty Fruit Bunches by Microwave Pre-treatment
Kasim, F., & Asben (2022) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/1059/1/012079 · Cited by 4
“Pretreatment of bagasse with a combination of alkali and microwave at 450 watts for 5 minutes, obtained 96% lignin removal [17].”
Value Added Products Generation from Sugarcane Bagasse and Its Impact on Economizing Biorefinery and Sustainability of Sugarcane Industry
Korasapati, R., Bastipati, S. B., Rosangzuala, K., Reddy, P. R., Raveena, G., Babu, P. R., Chandrasekhar, C., Malothu, R., Prakasham, R. S., & Banoth, L. (2023) · IntechOpen eBooks · doi:10.5772/intechopen.107472 · Cited by 4

Authors: Binod, P., Satyanagalakshmi, K., Sindhu, R., Janu, K. U., Sukumaran, R. K., & Pandey, A.
Affiliations: National Institute for Interdisciplinary Science and Technology (IN)
Corresponding author: Pandey, Ashok
ORCID: Binod, Parameswaran
Sindhu, Raveendran
Sukumaran, Rajeev K.
Pandey, Ashok
Venue: Renewable Energy
Publisher: Elsevier BV
Published: 2012-01
Volume: 37
Issue: 1
Pages: 109-116
ISSN: 0960-1481 (ISSN-L)
DOI: 10.1016/j.renene.2011.06.007
OpenAlex: W1996438954
Cited by: 400
References: 41
Citations by year: 2026: 13 · 2025: 16 · 2024: 29 · 2023: 17 · 2022: 32 · 2021: 31 · 2020: 36 · 2019: 32 · 2018: 29 · 2017: 40 · 2016: 29 · 2015: 28 · 2014: 27 · 2013: 24 · 2012: 15
FWCI: 12.22
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
doi.org/10.15223/policy-017 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-012 (stm-asf)
doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-004 (stm-asf)
Locations: Renewable Energy (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Funders: Bangladesh Council of Scientific and Industrial Research
Department of Science and Technology, Ministry of Science and Technology, India
Technology Information, Forecasting and Assessment Council
Topics: Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
Physical Sciences › Energy › Fuel Technology › Thermochemical Biomass Conversion Processes
Keywords: microwave pretreatment, sugarcane bagasse, enzymatic saccharification, lignocellulosic biomass, bioethanol production, reducing sugar yield, lignin removal, alkaline pretreatment, acid pretreatment

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Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse

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