Characterization of a mycothiol ligase mutant of Rhodococcus jostii RHA1
Open accessAbstract
Mycothiol (1d-myo-inosityl 2-[N-acetyl-L-cysteinyl]amido-2-deoxy-alpha-D-glucopyranoside) is an important microbial thiol present only in actinomycetes. Rhodococcus jostii RHA1 degrades a wide range of xenobiotics, including polychlorinated biphenyls, nitriles and N-nitrosodimethylamine. Analyses revealed that this strain produces two thiols, mycothiol and ergothioneine, found in the other actinomycetes. A mycothiol ligase mutant strain of R. jostii RHA1 deficient in the production of mycothiol was constructed. This mutant has a number of interesting characteristics: (a) it overproduces the intermediate glucosamine-inositol (1-O-(2-amino-1-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol); (b) it is deficient in the biochemical degradation of a number of xenobiotics metabolized by the parent strain; (c) it shows increased susceptibility to a number of antibiotics; and (d) it shows unusual growth characteristics, exhibiting a long lag phase before normal exponential growth. The diverse phenotypes of the mutant indicate the utility of R. jostii RHA1 as a model for deciphering the various functions of mycothiol.
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ERG is synthesised by a variety of microbes, especially fungi and actinobacteria.
“The chief organisms capable of its synthesis are fungi and certain yeasts(59,60), though actinobacteria and certain other micro-organisms(60–66), including the slime mould Physarum polycephalum (65), cyanobacteria(67–71) and methylotrophs(72) are also naturally capable of its production.”
From The biology of ergothioneine, an antioxidant nutraceutical (2020) · cites this paper as [62]
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Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902 · see citing works
Gust, B., Challis, G. L., Fowler, K., Kieser, T., & Chater, K. F. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337542100 · Cited by 1,416 · see citing works
Cellular glutathione and thiols metabolism
Dickinson, D. A., & Forman, H. J. (2002) · Biochemical Pharmacology · doi:10.1016/s0006-2952(02)01172-3 · Cited by 852 · see citing works
The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse
McLeod, M. P., Warren, R. M., Hsiao, W., Araki, N., Myhre, M., Fernandes, C., Miyazawa, D., Wong, W., Lillquist, A. L., Wang, D. Y. Q., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0607048103 · Cited by 645 · see citing works
Self-citationDistribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes
Newton, G. L., Arnold, K., Price, M. S., Sherrill, C., Delcardayre, S. B., Aharonowitz, Y., Cohen, G., Davies, J., Fahey, R. C., & Davis, C. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.7.1990-1995.1996 · Cited by 523 · see citing works
Self-citationMechanisms of action of isoniazid
Timmins, G. S., & Deretic, V. P. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05467.x · Cited by 425 · see citing works
Paget, M. S. B., Chamberlin, L. C. L., Atrih, A., Foster, S. J., & Buttner, M. J. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.1.204-211.1999 · Cited by 403 · see citing works
Fahey, R. C., & Newton, G. L. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)43016-4 · Cited by 380 · see citing works
Self-citationTHE BIOLOGY AND GENETICS OF THE GENUS RHODOCOCCUS
Finnerty, W. R. (1992) · Annual Review of Microbiology · doi:10.1146/annurev.mi.46.100192.001205 · Cited by 279 · see citing works
A Novel Transformation of Polychlorinated Biphenyls by Rhodococcus sp. Strain RHA1
Seto, M., Kimbara, K., Shimura, M., Hatta, T., Fukuda, M., & Yano, K. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.9.3353-3358.1995 · Cited by 263 · see citing works
Rawat, M., Newton, G. L., Ko, M., Martinez, G. J., Fahey, R. C., & Av‐Gay, Y. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.11.3348-3355.2002 · Cited by 196 · see citing works
Self-citationBuchmeier, N. A., Newton, G. L., Koledin, T., & Fahey, R. C. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03416.x · Cited by 181 · see citing works
Self-citationNewton, G. L., Av‐Gay, Y., & Fahey, R. C. (2000) · Biochemistry · doi:10.1021/bi000356n · Cited by 178 · see citing works
Self-citationBiological Chemistry of Naturally Occurring Thiols of Microbial and Marine Origin
Hand, C. E., & Honek, J. F. (2005) · Journal of Natural Products · doi:10.1021/np049685x · Cited by 158 · see citing works
Catabolism of Benzoate and Phthalate in Rhodococcus sp. Strain RHA1: Redundancies and Convergence
Patrauchan, M. A., Florizone, C., Dosanjh, M., Mohn, W. W., DAVIES, J. E., & Eltis, L. D. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.12.4050-4063.2005 · Cited by 152 · see citing works
Self-citationMycothiol Is Essential for Growth of Mycobacterium tuberculosis Erdman
Sareen, D., Newton, G. L., Fahey, R. C., & Buchmeier, N. A. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.22.6736-6740.2003 · Cited by 145 · see citing works
Self-citationVetting, M. W., Hegde, S. S., Javid-Majd, F., Blanchard, J. S., & Roderick, S. L. (2002) · Nature Structural Biology · doi:10.1038/nsb830 · Cited by 137 · see citing works
Newton, G. L., Bewley, C. A., Dwyer, T. J., Horn, R., Aharonowitz, Y., Cohen, G., DAVIES, J. E., Faulkner, D. J., & Fahey, R. C. (1995) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1995.0821h.x · Cited by 122 · see citing works
Self-citationSharp, J. O., Sales, C. M., LeBlanc, J. C., Liu, J., Wood, T. K., Eltis, L. D., Mohn, W. W., & Alvarez‐Cohen, L. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.01697-07 · Cited by 120 · see citing works
delCardayré, S. B., Stock, K. P., Newton, G. L., Fahey, R. C., & DAVIES, J. E. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5744 · Cited by 116 · see citing works
Self-citationMycothiol-dependent proteins in actinomycetes
Rawat, M., & Av‐Gay, Y. (2007) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2006.00062.x · Cited by 103 · see citing works
Koledin, T., Newton, G. L., & Fahey, R. C. (2002) · Archives of Microbiology · doi:10.1007/s00203-002-0462-y · Cited by 101 · see citing works
Self-citationNewton, G. L., Unson, M. D., Anderberg, S. J., Aguilera, J. A., Oh, N. N., delCardayré, S. B., Av‐Gay, Y., & Fahey, R. C. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0156 · Cited by 97 · see citing works
Self-citationSulfate Metabolism in Mycobacteria
Schelle, M. W., & Bertozzi, C. R. (2006) · ChemBioChem · doi:10.1002/cbic.200600224 · Cited by 91 · see citing works
Roles of Ring-Hydroxylating Dioxygenases in Styrene and Benzene Catabolism inRhodococcus jostiiRHA1
Patrauchan, M. A., Florizone, C., Eapen, S., Gómez-Gil, L., Sethuraman, B., Fukuda, M., DAVIES, J. E., Mohn, W. W., & Eltis, L. D. (2007) · Journal of Bacteriology · doi:10.1128/jb.01122-07 · Cited by 86 · see citing works
Self-citationTranscriptomic Assessment of Isozymes in the Biphenyl Pathway of Rhodococcus sp. Strain RHA1
Gonçalves, E. R., Hara, H., Miyazawa, D., DAVIES, J. E., Eltis, L. D., & Mohn, W. W. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.00947-06 · Cited by 79 · see citing works
Self-citationMahenthiralingam, E., Marklund, B., Brooks, L. A., Smith, D. A., Bancroft, G. J., & Stokes, R. W. (1998) · Infection and Immunity · doi:10.1128/iai.66.8.3626-3634.1998 · Cited by 78 · see citing works
Biochemistry of the Initial Steps of Mycothiol Biosynthesis
Newton, G. L., Ta, P., Bzymek, K. P., & Fahey, R. C. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m604724200 · Cited by 78 · see citing works
Self-citationFeng, J., Che, Y., Milse, J., Yin, Y., Liu, L., Rückert, C., Shen, X., Qi, S.-W., Kalinowski, J., & Liu, S. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m513192200 · Cited by 77 · see citing works
Park, J., & Roe, J. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06191.x · Cited by 74 · see citing works
Mycothiol Biosynthesis and Metabolism
Anderberg, S. J., Newton, G. L., & Fahey, R. C. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30391 · Cited by 72 · see citing works
Self-citationWarren, R. M., Hsiao, W., Kudo, H., Myhre, M., Dosanjh, M., Petrescu, A. S., Kobayashi, H., Shimizu, S., Miyauchi, K., Masai, E., et al. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.22.7783-7795.2004 · Cited by 69 · see citing works
Self-citationFirst Total Synthesis of Mycothiol and Mycothiol Disulfide
Lee, S., & Rosazza, J. P. N. (2004) · Organic Letters · doi:10.1021/ol0362008 · Cited by 66 · see citing works
Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1
Okamoto, S., & Eltis, L. D. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.05834.x · Cited by 65 · see citing works
Shortcut to Mycothiol Analogues
Knapp, S., González, S., Myers, D. S., Eckman, L. L., & Bewley, C. A. (2002) · Organic Letters · doi:10.1021/ol0269796 · Cited by 45 · see citing works
Bzymek, K. P., Newton, G. L., Ta, P., & Fahey, R. C. (2007) · Journal of Bacteriology · doi:10.1128/jb.00644-07 · Cited by 31 · see citing works
Self-citationIdentification of genes for mycothiol biosynthesis in Streptomyces coelicolor A3(2).
Park, J., Cha, C., & Roe, J. (2006) · PubMed · Cited by 31 · see citing works
Microbial products. IV. X-14847, a new aminoglycoside from Micromonospora echinospora.
Maehr, H., LIU, C.-M., HERMANN, T. E., PROSSER, B. L. T., Smallheer, J. M., & Palleroni, N. J. (1980) · The Journal of Antibiotics · doi:10.7164/antibiotics.33.1431 · Cited by 14 · see citing works
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The biology of ergothioneine, an antioxidant nutraceutical
Borodina, I., Kenny, L. C., McCarthy, C. M., Paramasivan, K., Pretorius, E., Roberts, T. J., van der Hoek, S. A., & Kell, D. B. (2020) · Nutrition Research Reviews · doi:10.1017/s0954422419000301 · Cited by 296 · see citing works
Glutathione analogs in prokaryotes.
Fahey, R. C. (2013) · Biochimica et Biophysica Acta · doi:10.1016/j.bbagen.2012.10.006 · Cited by 205 · see citing works
Shen, X., Zhou, N., & Liu, S. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4139-4 · Cited by 133 · see citing works
Developmental biology ofStreptomycesfrom the perspective of 100 actinobacterial genome sequences
Chandra, G., & Chater, K. F. (2013) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12047 · Cited by 132 · see citing works
Ta, P., Buchmeier, N. A., Newton, G. L., Rawat, M., & Fahey, R. C. (2011) · Journal of Bacteriology · doi:10.1128/jb.01402-10 · Cited by 78 · see citing works
Self-citationCombination of degradation pathways for naphthalene utilization in R hodococcus sp. strain TFB
Tomás‐Gallardo, L., Gómez‐Álvarez, H., Santero, E., & Floriano, B. (2013) · Microbial Biotechnology · doi:10.1111/1751-7915.12096 · Cited by 45 · see citing works
Liu, T., Xu, Y., Liu, H., Luo, S., Yin, Y., Liu, S., & Zhou, N. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-3033-1 · Cited by 39 · see citing works
Sulfur metabolism in Rhodococcus species and their application in desulfurization of fossil fuels
Hou, J., Deng, H., Liu, Z., Xu, P., & Wang, L. (2023) · Journal of Applied Microbiology · doi:10.1093/jambio/lxad048 · Cited by 25 · see citing works
Costa, J. S. D., Silva, R. A., Leichert, L. I. O., & Álvarez, H. M. (2017) · Microbiology · doi:10.1099/mic.0.000424 · Cited by 21 · see citing works
Broadley, S. G., Gumbart, J. C., Weber, B. W., Marakalala, M. J., Steenkamp, D. J., & Sewell, B. T. (2012) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s090744491203449x · Cited by 17 · see citing works
Lei, Z., Wang, Z., Zhang, H., Jia, Y., Zhang, D., Shi, R., Wang, C., & Chen, W. (2025) · Food & Function · doi:10.1039/d5fo02337h · Cited by 18 · see citing works
Firrincieli, A., Zannoni, D., Donini, E., Dostálová, H., Rädisch, R., Iommarini, L., Turner, R. J., Busche, T., Pátek, M., & Cappelletti, M. (2022) · Applied and Environmental Microbiology · doi:10.1128/aem.02209-21 · Cited by 13 · see citing works
L-cysteine:1d-myo-inositol 2-amino-2-deoxy-α-d-glucopyranoside ligase 6.3.1.13
Schomburg, D., & Schomburg, I. (2013) · Springer eBooks · doi:10.1007/978-3-642-36260-6_87
- Authors
- Dosanjh, M., Newton, G. L., & Davies, J.
- Affiliations
- University of British Columbia (CA)
- University of California San Diego (US)
- Corresponding author
- Davies, Julian
- ORCID
- Davies, Julian
- Venue
- Research in Microbiology
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 159
- Issue
- 9-10
- Pages
- 643-650
- ISSN
- 0769-2609 (ISSN-L)
- 0923-2508
- OpenAlex
- W1979838277
- Cited by
- 13
- References
- 39
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2020: 1 · 2017: 1 · 2013: 3 · 2012: 3
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 5499 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Research in Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Natural Sciences and Engineering Research Council of Canada
- National Institute of Allergy and Infectious Diseases (AI 072133, R01AI072133)
- Topics
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- mycothiol, Rhodococcus jostii RHA1, ergothioneine, actinomycetes, xenobiotic degradation, polychlorinated biphenyls, N-nitrosodimethylamine, antibiotic susceptibility, lag phase, mutant strain
- MeSH terms
- Bacterial Proteins, Culture Media, Cysteine, Ergothioneine, Glucosamine, Glycopeptides, Inositol, Ligases, Mutation, Rhodococcus, Gene Deletion
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