Oxidative stress time course in the rat diaphragm after freezing–thawing cycles
Abstract
Hyperthermia was shown to induce oxidative stress by uncoupling mitochondrial respiratory chain and to reduce superoxide dismutase (SOD) activity in muscles. Reactive carbonyl groups, malondialdehyde (MDA)-protein adducts, 3-nitrotyrosine immunoreactivity, Mn-SOD, and catalase were detected using immunoblotting in rat diaphragm specimens and homogenates thawed at room temperature (after previous storage at -80 degrees C) for 5, 15, 30, and 60 min, and 3, 6, and 24h to be subsequently and immediately stored at -80 degrees C. Mn-SOD activity was also measured in all muscles. Both total protein carbonylation (reactive carbonyl groups and MDA-protein adducts) and nitration were significantly increased over time, reaching their peaks in the diaphragms of the 60- and 15-min groups, respectively. Mn-SOD expression and activity were significantly reduced over time, while catalase expression showed no significant variation. Protein oxidation was significantly increased in the rat diaphragms exposed to freezing-thawing cycles of different time lengths, while Mn-SOD was substantially reduced in all muscles.
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UCP3 expression in cultured myotubes and rodent skeletal muscle has been linked to modulation of fatty acid oxidation, mitochondrial integrity, and management of ROS/oxidative stress.
“its expression in cultured myotubes and rodent skeletal muscle has been linked to modulation of fatty acid oxidation [56–58], mitochondrial integrity, and to management of ROS/oxidative stress [59–62].”
From Proinflammatory NFkB signalling promotes mitochondrial dysfunction in skeletal muscle in response to cellular fuel overloading (2019) · cites this paper as [59]
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Nisr, R. B., Shah, D. S., Ganley, I. G., & Hundal, H. S. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03148-8 · Cited by 142 · see citing works
- Authors
- Sellares, J., Mas, S., Melo, E., Sánchez, F., Marin, J., Gea, J., & Barreiro, E.
- Affiliations
- Universitat Pompeu Fabra (ES)
- Corresponding author
- Barreiro, Esther
- Venue
- Respiratory Physiology & Neurobiology
- Publisher
- Elsevier BV
- Published
- 2007-02
- Volume
- 155
- Issue
- 2
- Pages
- 156-166
- ISSN
- 1569-9048 (ISSN-L)
- OpenAlex
- W2171299153
- Cited by
- 7
- References
- 39
- Citations by year
- 2024: 1 · 2019: 2
- FWCI
- 1.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Respiratory Physiology & Neurobiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
- Keywords
- oxidative stress, freeze-thaw cycles, diaphragm, protein carbonylation, nitration, nitrotyrosine, manganese superoxide dismutase, catalase, mitochondrial uncoupling, hyperthermia, rats
- MeSH terms
- Animals, Catalase, Diaphragm, Freezing, Gene Expression Regulation, Hypothermia, Immunohistochemistry, Male, Superoxide Dismutase, Time Factors, Tyrosine, Blotting, Western, Rats, Sprague-Dawley, Oxidative Stress, Rats, In Vitro Techniques
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