Cited by 7Exercise and Physiological ResponsesAntioxidant Activity and Oxidative StressMeat and Animal Product Quality

Oxidative stress time course in the rat diaphragm after freezing–thawing cycles

Sellares, Jacobo, Mas, Sandra, Melo, Esther, Sánchez, Francesc, Marin, Judith, Gea, Joaquim, Barreiro, Esther·2007·Respiratory Physiology & Neurobiology

Abstract

Hyperthermia was shown to induce oxidative stress by uncoupling mitochondrial respiratory chain and to reduce superoxide dismutase (SOD) activity in muscles. Reactive carbonyl groups, malondialdehyde (MDA)-protein adducts, 3-nitrotyrosine immunoreactivity, Mn-SOD, and catalase were detected using immunoblotting in rat diaphragm specimens and homogenates thawed at room temperature (after previous storage at -80 degrees C) for 5, 15, 30, and 60 min, and 3, 6, and 24h to be subsequently and immediately stored at -80 degrees C. Mn-SOD activity was also measured in all muscles. Both total protein carbonylation (reactive carbonyl groups and MDA-protein adducts) and nitration were significantly increased over time, reaching their peaks in the diaphragms of the 60- and 15-min groups, respectively. Mn-SOD expression and activity were significantly reduced over time, while catalase expression showed no significant variation. Protein oxidation was significantly increased in the rat diaphragms exposed to freezing-thawing cycles of different time lengths, while Mn-SOD was substantially reduced in all muscles.

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Crossref · 10.1016/j.resp.2006.05.008

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Authors
Sellares, J., Mas, S., Melo, E., Sánchez, F., Marin, J., Gea, J., & Barreiro, E.
Affiliations
Universitat Pompeu Fabra (ES)
Corresponding author
Barreiro, Esther
Venue
Respiratory Physiology & Neurobiology
Publisher
Elsevier BV
Published
2007-02
Volume
155
Issue
2
Pages
156-166
ISSN
1569-9048 (ISSN-L)
OpenAlex
W2171299153
Cited by
7
References
39
Citations by year
2024: 1 · 2019: 2
FWCI
1.12
Type
journal article
Language
en
Open access
closed
Locations
  • Respiratory Physiology & Neurobiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Meat and Animal Product Quality
Keywords
oxidative stress, freeze-thaw cycles, diaphragm, protein carbonylation, nitration, nitrotyrosine, manganese superoxide dismutase, catalase, mitochondrial uncoupling, hyperthermia, rats
MeSH terms
Animals, Catalase, Diaphragm, Freezing, Gene Expression Regulation, Hypothermia, Immunohistochemistry, Male, Superoxide Dismutase, Time Factors, Tyrosine, Blotting, Western, Rats, Sprague-Dawley, Oxidative Stress, Rats, In Vitro Techniques

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