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Cited by 46Constructed Wetlands for Wastewater TreatmentWastewater Treatment and Nitrogen RemovalWastewater Treatment and Reuse

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Unraveling the active microbial populations involved in nitrogen utilization in a vertical subsurface flow constructed wetland treating urban wastewater

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Pelissari, Catiane, Guivernau, Miriam, Viñas, Marc, de Souza, Samara Silva, García, Joan, Sezerino, Pablo Heleno, Ávila, Cristina·2017·Science of The Total Environment
DOI 10.1016/j.scitotenv.2017.01.091
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Abstract

The dynamics of the active microbial populations involved in nitrogen transformation in a vertical subsurface flow constructed wetland (VF) treating urban wastewater was assessed. The wetland (1.5m 2 ) operated under average loads of 130gCODm -2 d -1 and 17gTNm -2 d -1 in Period I, and 80gCODm -2 d -1 and 19gTNm -2 d -1 in Period II. The hydraulic loading rate (HLR) was 375mmd -1 and C/N ratio was 2 in both periods. Samples for microbial characterization were collected from the filter medium (top and bottom layers) of the wetland, water influent and effluent at the end of Periods I (Jun-Oct) and II (Nov-Jan). The combination of qPCR and high-throughput sequencing (NGS, MiSeq) assessment at DNA and RNA level of 16S rRNA genes and nitrogen-based functional genes (amoA and nosZ-clade I) revealed that nitrification was associated both with ammonia-oxidizing bacteria (AOB) (Nitrosospira) and ammonia-oxidizing archaea (AOA) (Nitrososphaeraceae), and nitrite-oxidizing bacteria (NOB) such as Nitrobacter. Considering the active abundance (based in amoA transcripts), the AOA population revealed to be more stable than AOB in both periods and depths of the wetland, being less affected by the organic loading rate (OLR). Although denitrifying bacteria (nosZ copies and transcripts) were actively detected in all depths, the denitrification process was low (removal of 2gTNm -2 d -1 for both periods) concomitant with NO x -N accumulation in the effluent. Overall, AOA, AOB and denitrifying bact…

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Metadata

Authors
Pelissari, C., Guivernau, M., Viñas, M., de Souza, S. S., García, J., Sezerino, P. H., & Ávila, C.
Affiliations
  • Universidade Federal de Santa Catarina (BR)
  • Institute of Agrifood Research and Technology (ES)
  • Universitat de Girona (ES)
Corresponding author
Ávila, Cristina
ORCID
Venue
Science of The Total Environment
Publisher
Elsevier BV
Published
2017-04
Volume
584-585
Pages
642-650
ISSN
0048-9697 (ISSN-L)
DOI
10.1016/j.scitotenv.2017.01.091
OpenAlex
W2585286569
Cited by
46
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 7 · 2021: 4 · 2020: 10 · 2019: 4 · 2018: 8 · 2017: 5
FWCI
7.70
Type
journal article
Language
en
Open access
Licence
Locations
  • The Science of The Total Environment (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (acceptedVersion, cc-by-nc-nd)
  • RECERCAT (Consorci de Serveis Universitaris de Catalunya) (acceptedVersion, other-oa)
  • UPCommons institutional repository (Universitat Politècnica de Catalunya) (submittedVersion, cc-by-nc-nd)
Funders
  • Ministry of Economy and Competitiveness (FJCI-2014-22245)
  • Coordination for the Improvement of Higher Level (CAPES-PDSE 99999.006475/2015-09)
Topics
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Constructed Wetlands for Wastewater Treatment
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
Keywords
vertical flow constructed wetlands, municipal wastewater treatment, nitrogen removal, nitrification, denitrification, ammonia-oxidizing archaea, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, nosZ, Nitrosospira, Nitrobacter, organic loading rate, hydraulic loading rate, microbial community dynamics, biofilm
MeSH terms
Ammonia, Archaea, Bacteria, Genes, Bacterial, Nitrogen, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S, Water Microbiology, Water Purification, Genes, Archaeal, Wetlands, Wastewater

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