Cited by 10,891Genomics and Phylogenetic StudiesMicrobial Community Ecology and PhysiologyEnvironmental DNA in Biodiversity Studies

Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB

Open access
DeSantis, T. Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E. L., Keller, K., Huber, T., Dalevi, D. +2 more·2006·Applied and Environmental Microbiology

Abstract

A 16S rRNA gene database ( http://greengenes.lbl.gov ) addresses limitations of public repositories by providing chimera screening, standard alignment, and taxonomic classification using multiple published taxonomies. It was found that there is incongruent taxonomic nomenclature among curators even at the phylum level. Putative chimeras were identified in 3% of environmental sequences and in 0.2% of records derived from isolates. Environmental sequences were classified into 100 phylum-level lineages in the Archaea and Bacteria .

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/aem.03006-05

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
DeSantis, T. Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E. L., Keller, K., Huber, T., Dalevi, D., Hu, P., & Andersen, G. L.
Affiliations
  • Lawrence Berkeley National Laboratory (US)
  • Joint Genome Institute (US)
  • Danish Genome Institute, Gustav Wieds vej 10 C, DK-8000 Aarhus C, Denmark
  • Baylor University (US)
  • University of California, Berkeley (US)
  • The University of Queensland (AU)
  • Chalmers University of Technology (SE)
Corresponding author
Andersen, G. L.
Venue
Applied and Environmental Microbiology
Published
2006-07
Volume
72
Issue
7
Pages
5069-5072
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W1988925586
Cited by
10,891
References
33
Citations by year
2026: 220 · 2025: 384 · 2024: 545 · 2023: 576 · 2022: 741 · 2021: 1058 · 2020: 1148 · 2019: 1084 · 2018: 1054 · 2017: 964 · 2016: 770 · 2015: 561 · 2014: 441 · 2013: 317 · 2012: 296
FWCI
36.66
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
  • University of North Texas Digital Library (University of North Texas) (submittedVersion)
  • ANU Open Research (Australian National University) (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
  • Chalmers Research (Chalmers University of Technology) (submittedVersion)
  • Lawrence Berkeley National Laboratory
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
Keywords
Greengenes, chimeric sequences, taxonomic classification, sequence alignment, angiotensin receptor blockers, bacterial taxonomy, archaeal taxonomy
MeSH terms
Archaea, Bacteria, Recombination, Genetic, RNA, Ribosomal, 16S, Software, Reproducibility of Results, Polymerase Chain Reaction, Sequence Alignment, Genes, rRNA, Databases, Nucleic Acid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review