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Cited by 115Microbial bioremediation and biosurfactantsSteroid Chemistry and BiochemistryEnvironmental Chemistry and Analysis

Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil

Zhao, Hai-Ming, Hu, Rui-Wen, Chen, Xue-Xue, Chen, Xue-Bin, Lü, Huixiong, Li, Yan-Wen, Li, Hui, Mo, Ce-Hui, Cai, Quan-Ying, Wong, Ming-Hung·2018·Science of The Total Environment
DOI 10.1016/j.scitotenv.2018.05.334
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Abstract

A novel bacterial strain designated as Rhodococcus pyridinivorans XB, capable of utilizing various endocrine disruptor phthalates or phthalic acid (PA) as sole source of carbon and energy, was isolated from activated sludge. Under the optimal culture conditions (pH 7.08, 30.4 °C, inoculum size (OD 600 nm ) of 0.6) obtained by response surface methodology, di-(2-ethylhexyl) phthalate (DEHP, 200 mg/L) could be degraded by strain XB with a removal rate of 98% within 48 h. Under the observation of an atomic force microscope, it was confirmed that DEHP did not inhibit the growth of strain XB which might produce some extracellular polymeric substances as a response to DEHP stress, resulting in rapid degradation of DEHP. At initial concentrations of 50-800 mg/L DEHP, its degradation curves were well fitted with the first-order kinetic model, and the half-life of DEHP degradation varied from 5.44 to 23.5 h. The degradation intermediates of DEHP were identified by both GC-MS and high performance liquid chromatography-time of flight-mass spectrometry (HPLC-TOF-MS). Significant up-regulation was observed for the relative expression levels of genes (i.e., phthalate hydrolase, PA 3,4-dioxygenase, protocatechuate 3,4-α and 3,4-β dioxygenase) involved in DEHP degradation determined by real-time quantitative PCR (RT-qPCR). A DEHP biodegradation pathway by strain XB was proposed based on the identified intermediates and the degrading genes. Bioaugmentation of DEHP-contaminated soils with stra…

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Authors
Zhao, H.-M., Hu, R.-W., Chen, X.-X., Chen, X.-B., Lü, H., Li, Y.-W., Li, H., Mo, C.-H., Cai, Q.-Y., & Wong, M.-H.
Affiliations
  • Jinan University (CN)
  • South China Agricultural University (CN)
Corresponding author
Cai, Quan-Ying
ORCID
Venue
Science of The Total Environment
Publisher
Elsevier BV
Published
2018-11
Volume
640-641
Pages
1121-1131
ISSN
0048-9697 (ISSN-L)
DOI
10.1016/j.scitotenv.2018.05.334
OpenAlex
W2807047751
Cited by
115
References
0
Citations by year
2026: 5 · 2025: 12 · 2024: 21 · 2023: 21 · 2022: 22 · 2021: 14 · 2020: 14 · 2019: 5 · 2018: 1
FWCI
3.19
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • The Science of The Total Environment (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (41573087, 41471265, 41773108)
  • NSFC-Guangdong Joint Fund (U1501233)
  • Natural Science Foundation of Guangdong Province (2016A030312009)
  • Guangdong Science and Technology Department (2016B020242005, 2015B020235008)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
  • Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
Keywords
DEHP, Rhodococcus pyridinivorans, bioaugmentation, degradation intermediates, endocrine disruptors, phthalic acid, first-order degradation kinetics, extracellular polymeric substances, gene expression
MeSH terms
Biodegradation, Environmental, Diethylhexyl Phthalate, Phthalic Acids, Rhodococcus, Soil, Soil Pollutants

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