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Cited by 507

The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.

Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers

Open access
Rimoldi, Sara Giordana, Stefani, Fabrizio, Gigantiello, Anna, Polesello, Stefano, Comandatore, Francesco, Mileto, Davide, Maresca, Mafalda, Longobardi, Concetta, Mancon, Alessandro, Romeri, Francesca, Pagani, Cristina, Cappelli, Francesca, Roscioli, Claudio, Moja, Lorenzo, Gismondo, Maria Rita, Salerno, Franco·2020·Science of The Total Environment

Abstract

The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies are still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samples within the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA detection by means of real time RT-PCR and infectivity test on culture cells. SARS-CoV-2 RNA was detected in raw, but not in treated wastewaters (four and two samples, respectively, sampled in two dates). The isolated virus genome was sequenced, and belonged to the strain most spread in Europe and similar to another found in the same region. RNA presence in raw wastewater samples decreased after eight days, probably following the epidemiological trend estimated for the area. Virus infectivity was always null, indicating the natural decay of viral pathogenicity in time from emission. Samples from receiving rivers (three sites, sampled in the same dates as wastewaters) showed in some cases a positivity to real time RT-PCR, probably due to non-treated, or inefficiently treated discharges, or to the combined sewage overflows. Nevertheless, also for rivers infectivity was null. Risks for public health should be limited, although a precautionary approach to risk assessment is here advocated, giving the preliminary nature of the presented data.

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Metadata

Authors
Rimoldi, S. G., Stefani, F., Gigantiello, A., Polesello, S., Comandatore, F., Mileto, D., Maresca, M., Longobardi, C., Mancon, A., Romeri, F., Pagani, C., Cappelli, F., Roscioli, C., Moja, L., Gismondo, M. R., & Salerno, F.
Venue
Science of The Total Environment
Publisher
Elsevier BV
Published
2020-11
Volume
744
Pages
140911
ISSN
0048-9697 (ISSN-L)
OpenAlex
W3042026480
Cited by
507
References
38
FWCI
13.46
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers. https://app.cochat.ai/papers/10.1016/j.scitotenv.2020.140911
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Rimoldi, S. G., Stefani, F., Gigantiello, A., Polesello, S., Comandatore, F., Mileto, D., Maresca, M., Longobardi, C., Mancon, A., Romeri, F., Pagani, C., Cappelli, F., Roscioli, C., Moja, L., Gismondo, M. R., & Salerno, F. (2020). Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers. Science of The Total Environment. https://doi.org/10.1016/j.scitotenv.2020.140911

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.