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Small Molecules Efficiently Direct Endodermal Differentiation of Mouse and Human Embryonic Stem Cells
Open accessAbstract
Summary An essential step for therapeutic and research applications of stem cells is the ability to differentiate them into specific cell types. Endodermal cell derivatives, including lung, liver and pancreas, are of interest for regenerative medicine, but efforts to produce these cells have been met with only modest success. In a screen of 4000 compounds, two cell permeable small molecules were indentified that direct differentiation of ESCs into the endodermal lineage. These compounds induce nearly 80% of ESCs to form definitive endoderm, a higher efficiency than that achieved with Activin A or Nodal, commonly used protein inducers of endoderm. The chemically induced endoderm expresses multiple endodermal markers, can participate in normal development when injected into the embryonic gut tube and can form pancreatic progenitors in vitro. The application of small molecules to differentiate mouse and human ESCs into endoderm, and pancreatic progenitors represents a step toward achieving a reproducible and efficient production of desired ES cell derivatives.
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Cited by
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Epigenetic protein families: a new frontier for drug discovery
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Metadata
- Authors
- Borowiak, M., Maehr, R., Chen, S., Chen, A. E., Tang, W., Fox, J. L., Schreiber, S. L., & Melton, D. A.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 4
- Issue
- 4
- Pages
- 348-358
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2055710769
- Cited by
- 417
- References
- 50
- FWCI
- 18.72
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Borowiak, M., Maehr, R., Chen, S., Chen, A. E., Tang, W., Fox, J. L., Schreiber, S. L., & Melton, D. A. (2009). Small Molecules Efficiently Direct Endodermal Differentiation of Mouse and Human Embryonic Stem Cells. Cell Stem Cell. https://doi.org/10.1016/j.stem.2009.01.014
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