The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Derivation of Embryonic Stem-Cell Lines from Human Blastocysts
Open accessAbstract
Embryonic stem cells have the unique ability to form all adult cell types.Harnessing this potential may provide a source of cells to replace those that are lost or impaired as a result of disease.Moreover, the derivation of human embryonic stem cells opens a unique window into the study of early human development.At present, approximately 15 human embryonic stem-cell lines are publicly available, and they vary considerably in their usefulness for research and the extent of their characterization (see http://stemcells.nih.gov/registry/index.asp).To promote further research with human embryonic stem cells, we sought to derive and characterize more fully cell lines that meet strict criteria for ease of manipulation, including enzymatic passage with trypsin, streamlined freezing and thawing procedures, well-defined culture mediums, and straightforward methods for in vitro differentiation.We report the derivation and characterization of 17 additional human embryonic stem cell lines.We obtained frozen cleavage-and blastocyststage human embryos, produced by in vitro fertilization for clinical purposes, after obtaining written informed consent and approval by a Harvard institutional review board.A total of 286 frozen and thawed cleaved embryos (6 to 12 cells each) were cultured to the blastocyst stage, and 58 frozen and thawed blastocysts were allowed to re-expand in culture, whereupon they were treated with Tyrode's solution to remove the zona pellucida, followed by immunosurgery to isolate inner cell masses. 1 Many of these embryos were of such poor quality that they did not develop or divide after thawing.Nevertheless, 97 inner cell masses were isolated, and 17 individual human embryonic stem-cell lines (HUES1 through HUES17) were derived according to published protocols that we modified in terms of medium composition, enzymatic dissociation, and pro-The New England
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Cites
Embryonic Stem Cell Lines Derived from Human Blastocysts
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Stochastic State Transitions Give Rise to Phenotypic Equilibrium in Populations of Cancer Cells
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Somatic coding mutations in human induced pluripotent stem cells
Gore, A., Li, Z., Fung, H.-L., Young, J. E., Agarwal, S., Antosiewicz-Bourget, J., Canto, I., Giorgetti, A., Israel, M. A., Kiskinis, E., et al. (2011) · Nature · doi:10.1038/nature09805 · Cited by 1,273
Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z. D., Ziller, M., Croft, G. F., Amoroso, M. W., Oakley, D. H., et al. (2011) · Cell · doi:10.1016/j.cell.2010.12.032 · Cited by 1,009
Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9
Paquet, D., Kwart, D., Chen, A., Sproul, A., Jacob, S., Teo, S., Olsen, K. M., Gregg, A., Noggle, S., & Tessier-Lavigne, M. (2016) · Nature · doi:10.1038/nature17664 · Cited by 951
Metadata
- Authors
- Cowan, C. A., Klimanskaya, I., McMahon, J., Atienza, J., Witmyer, J., Zucker, J. P., Wang, S., Morton, C. C., McMahon, A. P., Powers, D., & Melton, D. A.
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 2004-03-25
- Volume
- 350
- Issue
- 13
- Pages
- 1353-1356
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W1988865085
- Cited by
- 964
- References
- 19
- FWCI
- 30.83
- Type
- journal article
- Language
- en
- Open access
- bronze
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