The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
BRACHYURY and CDX2 Mediate BMP-Induced Differentiation of Human and Mouse Pluripotent Stem Cells into Embryonic and Extraembryonic Lineages
Open accessAbstract
BMP is thought to induce hESC differentiation toward multiple lineages including mesoderm and trophoblast. The BMP-induced trophoblast phenotype is a long-standing paradox in stem cell biology. Here we readdressed BMP function in hESCs and mouse epiblast-derived cells. We found that BMP4 cooperates with FGF2 (via ERK) to induce mesoderm and to inhibit endoderm differentiation. These conditions induced cells with high levels of BRACHYURY (BRA) that coexpressed CDX2. BRA was necessary for and preceded CDX2 expression; both genes were essential for expression not only of mesodermal genes but also of trophoblast-associated genes. Maximal expression of the latter was seen in the absence of FGF but these cells coexpressed mesodermal genes and moreover they differed in cell surface and epigenetic properties from placental trophoblast. We conclude that BMP induces human and mouse pluripotent stem cells primarily to form mesoderm, rather than trophoblast, acting through BRA and CDX2.
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Cites
New cell lines from mouse epiblast share defining features with human embryonic stem cells
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Cited by
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells
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Development of the human placenta
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Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture
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Metadata
- Authors
- Bernardo, A. S., Faial, T., Gardner, L., Niakan, K. K., Ortmann, D., Senner, C. E., Callery, E. M., Trotter, M. W., Hemberger, M., Smith, J. C., Bardwell, L., Moffett, A., & Pedersen, R. A.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2011-08
- Volume
- 9
- Issue
- 2
- Pages
- 144-155
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2092099264
- Cited by
- 386
- References
- 49
- FWCI
- 10.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Bernardo, A. S., Faial, T., Gardner, L., Niakan, K. K., Ortmann, D., Senner, C. E., Callery, E. M., Trotter, M. W., Hemberger, M., Smith, J. C., Bardwell, L., Moffett, A., & Pedersen, R. A. (2011). BRACHYURY and CDX2 Mediate BMP-Induced Differentiation of Human and Mouse Pluripotent Stem Cells into Embryonic and Extraembryonic Lineages. Cell Stem Cell. https://doi.org/10.1016/j.stem.2011.06.015
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
