The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
Open accessAbstract
SUMMARY Functional modeling of many adult epithelia is limited by the difficulty of maintaining relevant stem cell populations in culture. Here, we show that dual inhibition of SMAD signaling pathways enables robust expansion of primary epithelial basal cell populations. We found that TGFβ/BMP/SMAD pathway signaling is strongly activated in luminal and suprabasal cells of several epithelia, but suppressed in p63+ basal cells. In airway epithelium, SMAD signaling promotes differentiation, and its inhibition leads to stem cell hyperplasia. Using dual SMAD inhibition in a feeder-free culture system we were able to expand airway basal stem cells from multiple species. Expanded cells can produce functional airway epithelium that is physiologically responsive to clinically relevant drugs such as CFTR modulators. This approach is effective for clonal expansion of single human cells and for basal cell populations from epithelial tissues from all three germ layers, and may therefore be broadly applicable for modeling of epithelia.
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Cites
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
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p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
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p63 is a p53 homologue required for limb and epidermal morphogenesis
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Basal cells as stem cells of the mouse trachea and human airway epithelium
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“Cellular senescence, defined as an irreversible cessation of cell division, has been proposed as the major obstacle preventing the long-term expansion of adult epithelial stem cells (Kiyono et al., 1998; Walters et al., 2013).”
BackgroundEpidermal growth factor and the multiplication of cultured human epidermal keratinocytes
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Distinct stem cells contribute to mammary gland development and maintenance
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Cited by
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
Montoro, D. T., Haber, A. L., Biton, M., Vinarsky, V., Lin, B., Birket, S. E., Yuan, F., Chen, S., Leung, H. M., Villoria, J., et al. (2018) · Nature · doi:10.1038/s41586-018-0393-7 · Cited by 1,206
Self-citationLong‐term expanding human airway organoids for disease modeling
Sachs, N., Papaspyropoulos, A., Zomer‐van Ommen, D. D., Heo, I., Böttinger, L., Klay, D., Weeber, F., Huelsz‐Prince, G., Iakobachvili, N., Amatngalim, G. D., et al. (2019) · The EMBO Journal · doi:10.15252/embj.2018100300 · Cited by 1,082
Screening cell–cell communication in spatial transcriptomics via collective optimal transport
(2023) · doi:10.1038/s41592-022-01728-4 · Cited by 484
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Ravindra, N. G., Alfajaro, M. M., Gasque, V., Huston, N. C., Wan, H., Szigeti-Buck, K., Yasumoto, Y., Greaney, A. M., Habet, V., Chow, R. D., et al. (2021) · PLOS Biology · doi:10.1371/journal.pbio.3001143 · Cited by 258
Metadata
- Authors
- Mou, H., Vinarsky, V., Tata, P. R., Brazauskas, K., Choi, S. H., Crooke, A. K., Zhang, B., Solomon, G. M., Turner, B., Bihler, H., Harrington, J., Lapey, A., Channick, C., Keyes, C., Freund, A., Artandi, S., Mense, M., Rowe, S., Engelhardt, J. F., Hsu, Y.-C., & Rajagopal, J.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2016-08
- Volume
- 19
- Issue
- 2
- Pages
- 217-231
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2421964473
- Cited by
- 382
- References
- 40
- FWCI
- 14.90
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Mou, H., Vinarsky, V., Tata, P. R., Brazauskas, K., Choi, S. H., Crooke, A. K., Zhang, B., Solomon, G. M., Turner, B., Bihler, H., Harrington, J., Lapey, A., Channick, C., Keyes, C., Freund, A., Artandi, S., Mense, M., Rowe, S., Engelhardt, J. F., . . . Rajagopal, J. (2016). Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells. Cell Stem Cell. https://doi.org/10.1016/j.stem.2016.05.012
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
