The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
3D Culture Supports Long-Term Expansion of Mouse and Human Nephrogenic Progenitors
Open accessAbstract
SUMMARY Transit-amplifying nephron progenitor cells (NPCs) generate all of the nephrons of the mammalian kidney during development. Their limited numbers, poor in vitro expansion, and difficult accessibility in humans have slowed basic and translational research into renal development and diseases. Here, we show that with appropriate 3D culture conditions, it is possible to support long-term expansion of primary mouse and human fetal NPCs as well as NPCs derived from human induced pluripotent stem cells (iPSCs). Expanded NPCs maintain genomic stability, molecular homogeneity, and nephrogenic potential in vitro, ex vivo, and in vivo. Cultured NPCs are amenable to gene targeting and can form nephron organoids that engraft in vivo, functionally couple to the host’s circulatory system, and produce urine-like metabolites via filtration. Together, these findings provide a technological platform for studying human nephrogenesis, modeling and diagnosing renal diseases, and drug discovery.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Takasato, M., Er, P. X., Chiu, H. S., Maier, B., Baillie, G. J., Ferguson, C., Parton, R. G., Wolvetang, E. J., Roost, M. S., Chuva de Sousa Lopes, S. M., et al. (2015) · Nature · doi:10.1038/nature15695 · Cited by 1,627
Mae, S.-I., Shono, A., Shiota, F., Yasuno, T., Kajiwara, M., Gotoda-Nishimura, N., Arai, S., Sato-Otubo, A., Toyoda, T., Takahashi, K., et al. (2013) · Nature Communications · doi:10.1038/ncomms2378 · Cited by 291
SnapShot: Growing Organoids from Stem Cells
Sato, T., & Clevers, H. (2015) · Cell · doi:10.1016/j.cell.2015.06.028 · Cited by 168
Cited by
In vivo target gene activation via CRISPR/Cas9-mediated trans-epigenetic modulation
Liao, H.-K., Hatanaka, F., Araoka, T., Reddy, P., Wu, M.-Z., Sui, Y., Yamauchi, T., Sakurai, M., O’Keefe, D. D., Núñez-Delicado, E., et al. (2017) · Cell · doi:10.1016/j.cell.2017.10.025 · Cited by 451
Self-citationOrganoids — Preclinical Models of Human Disease
Li, M., & Izpisua Belmonte, J. C. (2019) · New England Journal of Medicine · doi:10.1056/nejmra1806175 · Cited by 355
Self-citationCao, J.-Y., Wang, B., Tang, T.-T., Wen, Y., Li, Z.-L., Feng, S.-T., Wu, M., Liu, D., Yin, D., Ma, K.-L., et al. (2021) · Theranostics · doi:10.7150/thno.54550 · Cited by 303
Self-citationThe potential of organoids in urological cancer research
Wang, S., Gao, D., & Chen, Y. (2017) · Nature Reviews Urology · doi:10.1038/nrurol.2017.65 · Cited by 87
Kidney Organoids as Disease Models: Strengths, Weaknesses and Perspectives
Romero-Guevara, R., Ioannides, A., & Xinaris, C. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.563981 · Cited by 65
Metadata
- Authors
- Li, Z., Araoka, T., Wu, J., Liao, H.-K., Li, M., Lazo, M., Zhou, B., Sui, Y., Wu, M.-Z., Tamura, I., Xia, Y., Beyret, E., Matsusaka, T., Pastan, I., Rodriguez Esteban, C., Guillen, I., Guillen, P., Campistol, J. M., & Izpisua Belmonte, J. C.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2016-10
- Volume
- 19
- Issue
- 4
- Pages
- 516-529
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2511728841
- Cited by
- 179
- References
- 4
- FWCI
- 10.53
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: 3D Culture Supports Long-Term Expansion of Mouse and Human Nephrogenic Progenitors. https://app.cochat.ai/papers/10.1016/j.stem.2016.07.016
- The paper
- Li, Z., Araoka, T., Wu, J., Liao, H.-K., Li, M., Lazo, M., Zhou, B., Sui, Y., Wu, M.-Z., Tamura, I., Xia, Y., Beyret, E., Matsusaka, T., Pastan, I., Rodriguez Esteban, C., Guillen, I., Guillen, P., Campistol, J. M., & Izpisua Belmonte, J. C. (2016). 3D Culture Supports Long-Term Expansion of Mouse and Human Nephrogenic Progenitors. Cell Stem Cell. https://doi.org/10.1016/j.stem.2016.07.016
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
