The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Mex3a Marks a Slowly Dividing Subpopulation of Lgr5+ Intestinal Stem Cells
Open accessAbstract
SUMMARY The intestinal epithelium is continuously regenerated by highly proliferative Lgr5+ intestinal stem cells (ISCs). The existence of a population of quiescent ISCs has been suggested yet its identity and features remain controversial. Here we describe that the expression of the RNA-binding protein Mex3a labels a subpopulation of Lgr5+ cells that divide less frequently and contribute to regenerate all intestinal lineages with slow kinetics. Single cell transcriptomic analysis revealed two classes of Lgr5-high cells, one of them defined by the Mex3a-expression program and by low levels of proliferation genes. Lineage tracing experiments show that large fraction of Mex3a+ cell population is continuously recalled into the rapidly dividing self-renewing ISC pool in homeostatic conditions. Chemotherapy and radiation target preferentially rapidly dividing Lgr5+ cells but spare the Mex3a-high/Lgr5+ population, which helps sustain the renewal of the intestinal epithelium during treatment.
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
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“Read one (12 bp) contained cell and unique molecular barcodes (Islam et al., 2014; 36 Kivioja et al., 2012); read two (85 bp) contained the transcript sequence.”
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Self-citationIntestinal label-retaining cells are secretory precursors expressing Lgr5
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Self-citationInterconversion Between Intestinal Stem Cell Populations in Distinct Niches
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Lgr5 stem cells are indispensable for radiation-induced intestinal regeneration
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The External RNA Controls Consortium: a progress report
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Pereira, B., Billaud, M., & Almeida, R. (2017) · Trends in Cancer · doi:10.1016/j.trecan.2017.05.003 · Cited by 773
Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell
Ayyaz, A., Kumar, S., Sangiorgi, B., Ghoshal, B., Gosio, J., Ouladan, S., Fink, M., Barutcu, S., Trcka, D., Shen, J., et al. (2019) · Nature · doi:10.1038/s41586-019-1154-y · Cited by 573
Benchmarking single-cell RNA-sequencing protocols for cell atlas projects
Mereu, E., Lafzi, A., Moutinho, C., Ziegenhain, C., McCarthy, D. J., Álvarez-Varela, A., Batlle, E., Sagar, Grün, D., Lau, J. K., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0469-4 · Cited by 499
Self-citationIntestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
Yan, K. S., Gevaert, O., Zheng, G. X. Y., Anchang, B., Probert, C. S., Larkin, K. A., Davies, P. S., Cheng, Z.-F., Kaddis, J. S., Han, A., et al. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.06.014 · Cited by 361
Metadata
- Authors
- Barriga, F. M., Montagni, E., Mana, M., Mendez-Lago, M., Hernando-Momblona, X., Sevillano, M., Guillaumet-Adkins, A., Rodriguez-Esteban, G., Buczacki, S. J. A., Gut, M., Heyn, H., Winton, D. J., Yilmaz, O. H., Attolini, C. S.-O., Gut, I., & Batlle, E.
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 20
- Issue
- 6
- Pages
- 801-816.e7
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- OpenAlex
- W2591975886
- Cited by
- 207
- References
- 12
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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