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iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome

Kosanke, Maike, Davenport, Colin, Szepes, Monika, Wiehlmann, Lutz, Kohrn, Tim, Dorda, Marie, Gruber, Jonas, Menge, Kaja, Sievert, Maike, Melchert, Anna, Gruh, Ina, Göhring, Gudrun, Martin, Ulrich·2021·Stem Cell Reports
DOI 10.1016/j.stemcr.2021.08.016PMC PMC8514965

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  • Individual PSCs display considerable heterogeneity in mutation loads that can impact their utilization in disease modeling and as drug screening platforms.

    “Furthermore, individual PSCs display considerable heterogeneity in mutation loads that can impact their utilization in disease modeling and as drug screening platforms [163,164].”

    From The Mitochondrial Genome in Aging and Disease and the Future of Mitochondrial Therapeutics (2022) · cites this paper as [164]

    The Introduction and Results report differing mitochondrial variants and heteroplasmy levels among iPSC clones, including potentially damaging variants. The Discussion says heteroplasmy levels can rise enough to affect cell functionality. Our copy does not establish an impact on using those cells for disease modeling or drug screening; “drug screening” appears only in a reference title. (LLM, conf=0.83)

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Cited by

Sorted by citations

Metadata

Authors
Kosanke, M., Davenport, C., Szepes, M., Wiehlmann, L., Kohrn, T., Dorda, M., Gruber, J., Menge, K., Sievert, M., Melchert, A., Gruh, I., Göhring, G., & Martin, U.
Venue
Stem Cell Reports
Publisher
Elsevier BV
Published
2021-10
Volume
16
Issue
10
Pages
2488-2502
ISSN
2213-6711
DOI
10.1016/j.stemcr.2021.08.016
PMC
PMC8514965
Cited by
17
References
2
Language
en
Licence
Funders
  • Deutsches Zentrum für Lungenforschung (82DZL00401, 82DZL00201)
  • Lower Saxony State Ministry of Science and Culture
  • Bundesministerium für Bildung und Forschung
  • Deutsche Forschungsgemeinschaft

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