Natural deep eutectic solvents in combination with ultrasonic energy as a green approach for solubilisation of proteins: application to gluten determination by immunoassay
Abstract
In this work, a fast and miniaturised procedure based on the use of a natural deep eutectic solvent (NADES) in combination with ultrasound-assisted extraction (UAE) has been proposed for gluten determination by a commercial enzyme-linked immunosorbent assay (ELISA). Fourteen NADESs were prepared by combining two natural primary metabolites and water. Studies on NADES viscosity and gluten solubilisation in NADESs and ethanol-water solutions (for comparison purposes) were carried out. Different strategies for speeding-up gluten solubilisation in NADESs were evaluated: dilution, temperature and sonication by a cup-horn sonoreactor. Diluted fructose-citric acid NADES and sonication were finally selected for gluten solubilisation. Solubilised proteins were characterized by electrophoresis and molecular fluorescence. The proposed procedure was also assessed in real samples, especially ultrasound time. Kit solvents (including reducing agents) were replaced by NADES, and hence, a reassessing of immunoassay system was necessary. Samples with and without gluten as well as recovery tests were used for this purpose. Recoveries were in the range of 79-106% and the repeatability, expressed as relative standard deviation, was better than 15%.
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Both UAE and MAE provide shorter extraction time, increased yields, and often improved extract quality.
“Both UAE and MAE provide shorter extraction time, increased yields, and often improved quality of the extract (Cui et al. 2015;Lores et al. 2017;Wei et al. 2015a;2015b), thus they are categorized under 'Green Extraction' techniques.”
From The perspectives of natural deep eutectic solvents in agri-food sector (2020) · cites this paper as Lores et al. 2017
Dilute fructose:citric acid gave the best gluten solubilization, and citric acid could prevent gluten oxidation.
“The results demonstrated that gluten was best solubilized in dilute fructose:citric acid with the additional advantage that citric acid could prevent gluten oxidation.”
From Green solvents from ionic liquids and deep eutectic solvents to natural deep eutectic solvents (2018) · cites this paper as [98]
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References 23 · Cited by 96
- Authors
- Lores, H., Romero, V., Costas, I., Bendicho, C., & Lavilla, I.
- Affiliations
- Universidade de Vigo (ES)
- Corresponding author
- Lavilla, I.
- Venue
- Talanta
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 162
- Pages
- 453-459
- ISSN
- 0039-9140 (ISSN-L)
- OpenAlex
- W2532070915
- Cited by
- 96
- References
- 23
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 5 · 2023: 13 · 2022: 6 · 2021: 10 · 2020: 12 · 2019: 14 · 2018: 17 · 2017: 6
- FWCI
- 4.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Talanta (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Spanish Ministry of Economy and Competitiveness (CTQ2012-32788, CTQ2015-68146-P)
- Topics
- Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Health Sciences › Medicine › Gastroenterology › Celiac Disease Research and Management
- Keywords
- natural deep eutectic solvents, ultrasound-assisted extraction, protein solubilization, sonication, ELISA, green chemistry
- MeSH terms
- Biological Products, Electrophoresis, Polyacrylamide Gel, Fructose, Glutens, Immunoassay, Solubility, Solvents, Sonication, Viscosity, Water, Reproducibility of Results, Citric Acid
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