Polymerase subunit gamma 2 affects porcine oocyte maturation and subsequent embryonic development
Abstract
Deoxyribonucleic acid polymerase subunit gamma (POLG) is an enzyme encoded by the mitochondrial Polg gene. Polymerase (DNA directed), gamma 2, accessory subunit, also known as POLG2, is involved in mitochondrial replication. In the present study, we examined the role of Polg2 in the maturation of porcine oocytes. After Polg2 knockdown, the mitochondrial DNA copy number was significantly (P < 0.05) lower than that in the control group. However, there was no decrease in mitochondrial membrane potential. The decrease in mitochondrial DNA copy number led to reductions in adenosine-5'-triphosphate content (P < 0.05) and the maturation rate (P < 0.05) of oocytes. Furthermore, in the Polg2-knockdown group, maturation-promoting factor activity was decreased (P < 0.05) and the percentage of oocytes displaying abnormal actin filaments and microtubules was significantly increased (P < 0.05). This likely led to the reduced development rate and number of cells per blastocyst in this group (P < 0.05). In conclusion, Polg2 seems to be critical for mitochondrial replication and regulation of adenosine-5'-triphosphate content and affects porcine oocyte maturation and subsequent embryonic development.
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Knockdown of porcine oocyte PolG2 causes an aging-like phenotype as evidenced by decreased production of ATP and maturation promoting factor (MPF), reducing the fraction of oocytes that reach MII stage.
“Knockdown of porcine oocyte PolG2 causes an aging-like phenotype as evidenced by decreased production of ATP and maturation promoting factor (MPF), reducing the fraction of oocytes that reach MII stage (Lee et al., 2015).”
From Role of the mitochondrial DNA replication machinery in mitochondrial DNA mutagenesis, aging and age-related diseases (2017) · cites this paper as Lee et al., 2015
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- Authors
- Lee, S.-K., Zhao, M.-H., Zheng, Z., Kwon, J.-W., Liang, S., Kim, S.-H., Kim, N.-H., & Cui, X.-S.
- Affiliations
- Chungbuk National University (KR)
- Corresponding author
- Cui, Xiang-Shun
- Venue
- Theriogenology
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 83
- Issue
- 1
- Pages
- 121-130
- ISSN
- 0093-691X (ISSN-L)
- OpenAlex
- W1999930246
- Cited by
- 21
- References
- 41
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 4 · 2016: 1 · 2015: 3
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Theriogenology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Next-Generation BioGreen 21 Program (PJ009594, PJ00956302, PJ00909801)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- POLG2, porcine oocyte maturation, mitochondrial DNA copy number, mitochondrial DNA replication, ATP levels, maturation-promoting factor, blastocyst development, oogenesis, gene knockdown
- MeSH terms
- Adenosine Triphosphate, Animals, DNA-Directed DNA Polymerase, Mitochondria, Oocytes, Parthenogenesis, Swine, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, In Vitro Oocyte Maturation Techniques
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