Kidney-on-a-Chip technology for drug-induced nephrotoxicity screening
Open accessAbstract
Improved model systems to predict drug efficacy, interactions, and drug-induced kidney injury (DIKI) are crucially needed in drug development. Organ-on-a-chip technology is a suitable in vitro system because it reproduces the 3D microenvironment. A kidney-on-a-chip can mimic the structural, mechanical, transport, absorptive, and physiological properties of the human kidney. In this review we address the application of state-of-the-art microfluidic culturing techniques, with a focus on culturing kidney proximal tubules, that are promising for the detection of biomarkers that predict drug interactions and DIKI. We also discuss high-throughput screening and the challenges for in vitro to in vivo extrapolation (IVIVE) that will need to be overcome for successful implementation.
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Reproduction of cellular structure and function is incomplete under static culture conditions.
“the reproduction of cellular structure and function is also incomplete”
From Material-dependent oxygen transport governs ischemia-reperfusion injury and drug response in a kidney microphysiological system (2026) · cites this paper as Wilmer et al., 2016
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- Authors
- Wilmer, M. J., Ng, C. P., Lanz, H. L., Vulto, P., Suter-Dick, L., & Masereeuw, R.
- Affiliations
- Radboud University Nijmegen (NL)
- Mimetas (Netherlands) (NL)
- Utrecht University (NL)
- Corresponding author
- Wilmer, Martijn J.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2016-02
- Volume
- 34
- Issue
- 2
- Pages
- 156-170
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2208642322
- Cited by
- 354
- References
- 5
- Citations by year
- 2026: 12 · 2025: 22 · 2024: 44 · 2023: 21 · 2022: 49 · 2021: 44 · 2020: 31 · 2019: 38 · 2018: 38 · 2017: 36 · 2016: 19
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- green
- dspace.library.uu.nl
- Licence
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Radboud Repository (Radboud University) (submittedVersion)
- Funders
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC/C013204/1)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Nephrology › Acute Kidney Injury Research
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- kidney-on-a-chip, drug-induced nephrotoxicity, organ-on-a-chip, proximal tubule, high-throughput screening, in vitro-in vivo extrapolation, drug safety prediction
- MeSH terms
- Drug Evaluation, Preclinical, Humans, Kidney, Models, Biological, Organ Culture Techniques, Microfluidic Analytical Techniques, Acute Kidney Injury
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