The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications
Open accessAbstract
Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
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Cites
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Cited by
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery by AAV Vectors
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Precise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387
Self-citationXu, Y., & Li, Z. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2020.08.031 · Cited by 404
The emerging and uncultivated potential of CRISPR technology in plant science
Zhang, Y., Malzahn, A. A., Sretenovic, S., & Qi, Y. (2019) · Nature Plants · doi:10.1038/s41477-019-0461-5 · Cited by 394
Metadata
- Authors
- Molla, K. A., & Yang, Y.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2019-10
- Volume
- 37
- Issue
- 10
- Pages
- 1121-1142
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2939811618
- Cited by
- 331
- References
- 19
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- bronze
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