Animal immunization, in vitro display technologies, and machine learning for antibody discovery
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Hybridoma technology has relatively long development times when animals must be immunized, compared to in vitro phage display technologies.
“including relatively long development times when animals must be immunized, compared to in vitro phage display technologies (Laustsen et al., 2021)”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Laustsen et al., 2021
The 'Antibodies May Derive from Plasma, Hybridomas, and Recombinant Cell Lines' section says hybridomas are derived from immunized animals, and Figure 1 shows immunization in the hybridoma workflow. Neither passage, nor the paper’s comparison of discovery strategies in Table 1, says hybridoma development takes longer than in vitro phage display. (LLM, conf=0.82)
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Cites
Direct Gene Transfer into Mouse Muscle in Vivo
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In silico proof of principle of machine learning-based antibody design at unconstrained scale
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Data‐driven evaluation of suitable immunogens for improved antibody selection
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“Shorter immunogens (50 residues or fewer) with disordered or unfolded regions at the N-or C-terminus and long coil stretches were more likely to generate successful antibodies.”
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- Authors
- Laustsen, A. H., Greiff, V., Karatt-Vellatt, A., Muyldermans, S., & Jenkins, T. P.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2021-12
- Volume
- 39
- Issue
- 12
- Pages
- 1263-1273
- ISSN
- 0167-7799
- Cited by
- 157
- References
- 8
- Language
- en
- Licence
- Funders
- Leona M. and Harry B. Helmsley Charitable Trust
- Villum Fonden (00025302)
- European Research Council (850974)
- Research Council of Norway
- Horizon 2020
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