Effects of soy isoflavones on 17β-estradiol-induced proliferation of MCF-7 breast cancer cells
Abstract
Based on the results of in vitro-experiments in practically estrogen-free media and in the absence of estrogen-beta receptors, soy isoflavones have been suspected to enhance proliferation of MCF-7 breast cancer cells. In this study the effects of soy isoflavones on MCF-7 cells were investigated in the presence and absence of estrogen, directly and in a metabolized form by testing sera of postmenopausal women supplemented with isoflavones. First, three concentrations of isoflavones (0.1, 1 and 10 mumol/l) were tested at increasing levels of 17-beta-estradiol ( 20 pM, isoflavones both tested directly and indirectly (metabolized) revealed significant anti-proliferative effects as well as in the proliferation and the gene chip assay. These findings emphasize the reported advantageous properties of isoflavones for postmenopausal women.
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According to Imhof, typical in vivo estrogen levels range from 50-400 pM, with even menopausal estrogen levels exceeding 20 pM, the concentration of genistein achieved through supplementation.
“According to Imhof, typical in vivo estrogen levels range from 50–400 pM, with even menopausal estrogen levels exceeding 20 pM, the concentration of genistein achieved through supplementation [18].”
From Soy, Red Clover, and Isoflavones and Breast Cancer: A Systematic Review (2013) · cites this paper as [18]
According to this line of reasoning, at these relative concentrations, the agonistic effects of estrogen would be expected to outweigh those of genistein.
“According to this line of reasoning, at these relative concentrations, the agonistic effects of estrogen would be expected to outweight those of genistein [18].”
From Soy, Red Clover, and Isoflavones and Breast Cancer: A Systematic Review (2013) · cites this paper as [18]
In a study of soy and MCF-7 breast cancer cell growth, Imhof et al observed "minor proliferation enhancing effects" that occurred "only at unphysiologically low estrogen levels".
“In a study of soy and MCF-7 breast cancer cell growth as assessed in the presence and absence of estradiol, Imhof et al observed “minor proliferation enhancing effects” [18] that occurred “only at unphysiologically low estrogen levels” [18].”
From Soy, Red Clover, and Isoflavones and Breast Cancer: A Systematic Review (2013) · cites this paper as [18]
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Soy, Red Clover, and Isoflavones and Breast Cancer: A Systematic Review
Fritz, H., Seely, D., Flower, G., Skidmore, B., Fernandes, R., Vadeboncoeur, S., Kennedy, D., Cooley, K., Wong, R., Sagar, S., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0081968 · Cited by 188 · see citing works
- Authors
- Imhof, M., Molzer, S., & Imhof, M.
- Affiliations
- Landesklinikum Korneuburg (AT)
- Corresponding author
- Imhof, Marianne
- Imhof, Martin
- ORCID
- Imhof, Martin
- Venue
- Toxicology in Vitro
- Publisher
- Elsevier BV
- Published
- 2008-09
- Volume
- 22
- Issue
- 6
- Pages
- 1452-1460
- ISSN
- 0887-2333 (ISSN-L)
- OpenAlex
- W2051310463
- Cited by
- 32
- References
- 0
- Citations by year
- 2025: 1 · 2021: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2014: 2 · 2013: 7 · 2012: 4
- FWCI
- 2.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology in Vitro (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- soy isoflavones, estradiol, MCF-7 cells, cell proliferation, estrogen receptor, postmenopausal women, isoflavone supplementation, gene expression profiling, antiproliferative activity, estrogen metabolism
- MeSH terms
- Breast Neoplasms, Dose-Response Relationship, Drug, Estradiol, Estrogens, Female, Gene Expression Regulation, Humans, Isoflavones, Glycine max, Postmenopause, Gene Expression Profiling, Cell Line, Tumor, Cell Proliferation
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