Carbon black nanoparticle exposure during middle and late fetal development induces immune activation in male offspring mice
Abstract
Increasing exposure to nanoparticles (NPs) has raised concerns regarding their health and safety profiles in humans and animals, especially in developing organisms, which may display increased sensitivity to NP toxicity. The present study examined the effects of gestational exposure to carbon black NP (CB-NP) on the development of the offspring immune system. Pregnant mice were exposed to CB-NP (95μg/kg body weight) by intranasal instillation on gestational days 9 and 15. The thymus and spleen were collected from their offspring mice on postnatal day (PND) 1, 3 and 5. Thymocyte and splenocyte phenotypes were examined by determining the expression of cell-surface molecules using flow cytometry. Gene expression in the thymus and spleen was examined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Prenatal exposure to CB-NP increased total thymocytes and their immunophenotypes (CD4(-)CD8(-) and CD4(+)CD8(+) cells). It also induced an increase in total lymphocytes, and CD4(-)CD8(-), particularly CD3(-)B220(-)cells, at PND 5 in the spleen of newborn male offspring, reflecting the stimulation of immature splenocytes. Furthermore, mRNA expression of genes related to the induction of peripheral tolerance (i.e. thymic Traf6) was upregulated. These data suggest that respiratory exposure to CB-NP during middle and late gestation may have allergic or inflammatory effects in male offspring, and may provide initial information on the potential developmental imm…
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The magnitude of lymphocyte alteration depended on the stage of gestation at exposure.
“Importantly, the magnitude of alteration depended on the stage of gestation fetuses were exposed, highlighting the importance in the timing and duration of exposure.”
From Air pollution and children’s health—a review of adverse effects associated with prenatal exposure from fine to ultrafine particulate matter (2021) · cites this paper as [105]
Gestational exposure to carbon black nanoparticles induced dysregulation of lymphocyte populations in offspring, indicating neonatal peripheral tolerance.
“El-Sayed et al. [105] also demonstrated gestational exposure to carbon black nanoparticles induced dysregulation of lymphocyte populations in offspring, indicating neonatal peripheral tolerance.”
From Air pollution and children’s health—a review of adverse effects associated with prenatal exposure from fine to ultrafine particulate matter (2021) · cites this paper as [105]
Cites
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Hougaard, K. S., Jensen, K. A., Nordly, P., Taxvig, C., Vogel, U., Saber, A. T., & Wallin, H. (2008) · Particle and Fibre Toxicology · doi:10.1186/1743-8977-5-3 · Cited by 132 · see citing works
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Johnson, N. M., Hoffmann, A. R., Behlen, J. C., Lau, C., Pendleton, D., Harvey, N., Shore, R., Li, Y., Chen, J., Tian, Y., et al. (2021) · Environmental Health and Preventive Medicine · doi:10.1186/s12199-021-00995-5 · Cited by 339 · see citing works
“[105] also demonstrated gestational exposure to carbon black nanoparticles induced dysregulation of lymphocyte populations in offspring, indicating neonatal peripheral tolerance.”
Background
- Authors
- El-Sayed, Y. S., Shimizu, R., Onoda, A., Takeda, K., & Umezawa, M.
- Affiliations
- Tokyo University of Science (JP)
- Corresponding author
- El-Sayed, Yasser S.
- Venue
- Toxicology
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 327
- Pages
- 53-61
- ISSN
- 0300-483X (ISSN-L)
- OpenAlex
- W2036847201
- Cited by
- 63
- References
- 2
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 7 · 2020: 8 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 9 · 2015: 6
- FWCI
- 3.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- carbon black nanoparticles, developmental immunotoxicity, fetal immune system development, thymocytes, splenocytes, TRAF6, peripheral tolerance, male offspring
- MeSH terms
- Animals, Body Weight, Female, Immune System, Male, Mice, Inbred ICR, Pregnancy, Sex Ratio, Maternal Exposure, Fetal Development, Mice, Soot, Nanoparticles
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