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Cited by 123Probiotics and Fermented FoodsAlcohol Consumption and Health EffectsGut microbiota and health

The DOI matches its Crossref record; 37 of the 38 references it cites were found in a registry or an index.

Lactobacillus rhamnosus GG supernatant promotes intestinal barrier function, balances T reg and T H 17 cells and ameliorates hepatic injury in a mouse model of chronic-binge alcohol feeding

Chen, Rui-Cong, Xu, Lan-Man, Du, Shan-Jie, Huang, Si-Si, Wu, He, Dong, Jia-Jia, Huang, Jian-Rong, Wang, Xiao-Dong, Feng, Wen-Ke, Chen, Yong-Ping·2016·Toxicology Letters
DOI 10.1016/j.toxlet.2015.11.019
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Abstract

Impaired intestinal barrier function plays a critical role in alcohol-induced hepatic injury, and the subsequent excessive absorbed endotoxin and bacterial translocation activate the immune response that aggravates the liver injury. Lactobacillus rhamnosus GG supernatant (LGG-s) has been suggested to improve intestinal barrier function and alleviate the liver injury induced by chronic and binge alcohol consumption, but the underlying mechanisms are still not clear. In this study, chronic-binge alcohol fed model was used to determine the effects of LGG-s on the prevention of alcoholic liver disease in C57BL/6 mice and investigate underlying mechanisms. Mice were fed Lieber-DeCarli diet containing 5% alcohol for 10 days, and one dose of alcohol was gavaged on Day 11. In one group, LGG-s was supplemented along with alcohol. Control mice were fed isocaloric diet. Nine hours later the mice were sacrificed for analysis. Chronic-binge alcohol exposure induced an elevation in liver enzymes, steatosis and morphology changes, while LGG-s supplementation attenuated these changes. Treatment with LGG-s significantly improved intestinal barrier function reflected by increased mRNA expression of tight junction (TJ) proteins and villus-crypt histology in ileum, and decreased Escherichia coli (E. coli) protein level in liver. Importantly, flow cytometry analysis showed that alcohol reduced Treg cell population while increased TH17 cell population as well as IL-17 secretion, which was reversed by LGG-s administration. In conclusion, our findings indicate that LGG-s is effective in preventing chronic-binge alcohol exposure-induced liver injury and shed a light on the importance of the balance of Treg and TH17 cells in the role of LGG-s application.

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Crossref · 10.1016/j.toxlet.2015.11.019

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Research works printed but not linked: 1

  1. Yuhua Wang, 2012, Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury, Am. J. Physiol. Gastrointest. Liver Physiol.

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Metadata

Authors
Chen, R.-C., Xu, L.-M., Du, S.-J., Huang, S.-S., Wu, H., Dong, J.-J., Huang, J.-R., Wang, X.-D., Feng, W.-K., & Chen, Y.-P.
Affiliations
Wenzhou Medical University (CN)
Corresponding author
Chen, Rui-Cong
ORCID
Feng, Wen-Ke
Venue
Toxicology Letters
Publisher
Elsevier BV
Published
2016-01
Volume
241
Pages
103-110
ISSN
0378-4274 (ISSN-L)
DOI
10.1016/j.toxlet.2015.11.019
OpenAlex
W2181249084
Cited by
123
References
38
Citations by year
2026: 3 · 2025: 12 · 2024: 9 · 2023: 12 · 2022: 29 · 2021: 9 · 2020: 10 · 2019: 13 · 2018: 15 · 2017: 10 · 2016: 1
FWCI
11.56
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Toxicology Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Science Foundation of Zhejiang Province (LY15H030017)
  • National Five-year Science and Technology Major Projects (2013ZX10005002-001)
  • National Natural Science Foundation (81370481)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Lactobacillus rhamnosus GG, probiotic supernatant, alcoholic liver disease, binge drinking, intestinal barrier function, tight junction proteins, regulatory T cells, Th17 cells, IL-17, Escherichia coli, hepatic steatosis, endotoxin, bacterial translocation, gut-liver axis
MeSH terms
Animals, Chronic Disease, Diet, Fatty Liver, Alcoholic, Intestinal Absorption, Intestines, Liver, Liver Diseases, Alcoholic, Male, Mice, Inbred C57BL, Interleukin-17, Escherichia coli Proteins, T-Lymphocytes, Regulatory, Mice, Lacticaseibacillus rhamnosus, Th17 Cells, Tight Junction Proteins, Binge Drinking

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