Cited by 529CRISPR and Genetic EngineeringGenetically Modified Organisms ResearchRNA regulation and disease

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CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

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Manghwar, Hakim, Lindsey, Keith, Zhang, Xianlong, Jin, Shuangxia·2019·Trends in Plant Science

DOI 10.1016/j.tplants.2019.09.006

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Abstract

Genome editing (GE) has revolutionized biological research through the new ability to precisely edit the genomes of living organisms. In recent years, various GE tools have been explored for editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use, and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and Cas12a (Cpf1), are also considered.

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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116 · cited 1×
“corresponds to an NGG consensus sequence which contains two G:C base pairs [104]”
Background
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · cited 2×
“introduces in vitro dsDNA breaks at a specific site containing a sequence complementary to crRNA [45]”
Background
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062 · cited 5×
“There are several BE systems that have been used for editing living organisms, including BE3 [32], BE4 [33], Targeted-AID [22], and dCpf1-BE [34].”
BackgroundMethodInfluential
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761 · cited 1×
“The Francisella novicida U112 (FnCpf1) is analogous to CRISPR/Cas9 but exhibits some unique characteristics [17].”
Background
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741 · cited 1×
“The secondary structure of the RNA is a fundamental parameter for tuning the activity of various CRISPR systems, and gRNA structures can affect the cleavage of on- and off-target sites [41].”
Background
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V. K., Donghia, N. M., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,887 · cited 1×
“Cas13 was used to direct ADAR2 deaminase for the modification of RNA (changing adenosine to inosine) in human cells for the recovery of functional proteins to halt disease progression [7].”
Method
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
In vivo genome editing using Staphylococcus aureus Cas9
Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., et al. (2015) · Nature · doi:10.1038/nature14299 · Cited by 2,687

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Structural and functional bases of F. rodentium Cas9 provide insights into CRISPR-Cas protein engineering
Yang, M. J., Liu, S., Chen, G., Liu, X., Sun, D., Zhang, J., Wang, Y., Chen, S., Tian, R., & Hu, Z. (2025) · Cell Genomics · doi:10.1016/j.xgen.2025.101039 · Cited by 1
“8,9 Second, the effectiveness of SpCas9, a popular CRISPR-Cas9 enzyme, is limited by its need for a specific protospacer adjacent motif (PAM) sequence ‘‘NGG’’ near the target site.”
Development of an RT-ERA-CRISPR/Cas12a assay for duck Tembusu virus
Huang, Z., Ding, Y., Xu, H., Zhang, Q., Xiyi, W., Ding, H., Zhang, J., Wang, Y., Wang, Y., & Jiao, P. (2026) · Poultry Science · doi:10.1016/j.psj.2026.106612 · Cited by 1
“CRISPR/Cas12a (Cpf1) is a class II CRISPR system that recognizes target double-stranded DNA (dsDNA) sequences via a protospacer adjacent motif (5 ′ -TTTV-3 ′ ), guided by specific CRISPR RNA (crRNA) (Manghwar et al ., 2019; Bandyopadhyay et al ., 2020; Xu and Wu, 2026).”
Advances in stem cell therapy, nanomedicine, multi-omics, and artificial intelligence–assisted strategies for the treatment of lung injury and pulmonary fibrosis
Song, D., Yang, Y., Li, Z., Song, L., An, B., Yuan, Z., Liu, Y., Su, R., Guo, W., & Xie, L. (2026) · Letters in Drug Design & Discovery · doi:10.1016/j.lddd.2026.100370 · Cited by 1
Analytical Assessment of sgRNA Impurities and Their Impact on Functional Performance
Chatla, K., Ayalew, L., Yim, M., Ko, P., Lippold, S., Hernandez, G. E., Chua, B. A., Patil, D. P., & Camperi, J. (2026) · Analytical Chemistry · doi:10.1021/acs.analchem.6c00747 · Cited by 1
Visual detection platform based on RPA-CRISPR/Cas12a for Klebsiella pneumoniae and Carbapenem-resistant Klebsiella pneumoniae in clinical and food safety settings
Ji, T., Fang, X., Gao, Y. Z., Yu, K., He, J., Wang, L. Y., Zhu, W., Huang, G., & Gao, X. Z. (2026) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2026.1817859 · Cited by 1
“This system enables crRNA-guided Cas12a to recognize and cleave target DNA, with speci ﬁ city dependent on a TTTV-containing protospacer adjacent motif (PAM) (V = A, C, or G) and the protospacer (Manghwar et al., 2019).”
Recent advances in genome editing technologies for improvement of ornamental crops and their regulatory perspectives
Garg, D., Singh, T., & Bansal, S. (2026) · Discover Life · doi:10.1007/s11084-026-09732-w · Cited by 1
“(b)) [1].”
CRISPR-genome editing: Revolutionizing beneficial Bacteria and Fungi for sustainable agriculture
Mascarin, G. M., dos Santos, M. S. N., & Côrtes, M. V. D. C. B. (2026) · Current Research in Biotechnology · doi:10.1016/j.crbiot.2026.100404 · Cited by 1
Spatial and Multiple Omics Approaches for Pest and Disease Management: Opportunities and Challenges
Antwi-Boasiako, A., Amponsah, P., Adomako, J., Frimpong‐Anin, K., Ohene-Mensah, G., Boateng, A. B., Dormatey, R., Baafi, E., Annang, E. A., & Coulibaly, D. (2026) · CABI biotechnology series. · doi:10.1079/9781836994572.0014 · Cited by 1
CRISPR/Cas based electrochemical biosensors
Wu, T., Wu, Y., Gao, H., Huang, H., & Liu, Y. (2026) · Journal of Electroanalytical Chemistry · doi:10.1016/j.jelechem.2026.120386 · Cited by 1
CRISPR Technology - Recent Advances
Chen, Y. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.102156 · Cited by 2
Transcriptional Effects of Rootstock on Scion after Drought: A Case Study of Using MdGH3 RNAi as the Rootstock
He, J., Guo, J., Jiang, L., An, W., Ma, F., Guan, Q., & Niu, C. (2022) · Horticulturae · doi:10.3390/horticulturae8121212 · Cited by 1
Introductory Chapter: CRISPR Technology
Chen, Y. (2023) · IntechOpen eBooks · doi:10.5772/intechopen.107829 · Cited by 1
Insights into Grapevine Defence Response Against Fungal and Oomycete Diseases Towards a Sustainable Plant Breeding
Patanita, M., Félix, M. D. R., Ribeiro, J. A., Varanda, C. M. R., Albuquerque, A., Materatski, P., Garrido, N., & Campos, M. D. (2023) · Springer eBooks · doi:10.1007/978-981-99-4890-1_5 · Cited by 1
CRISPR/Cas systems and techniques
Poonia, A. K., Batra, B., Lal, M. K., & Gahlaut, V. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-18444-4.00003-x · Cited by 1
Predictability of Off-Targets in CRISPR-Cas9 Gene Editing Systems using Convolutional Neural Networks
Verma, P., Kethar, J., & Appavu, R. (2023) · Journal of Student Research · doi:10.47611/jsrhs.v12i3.5052 · Cited by 1
Genome Editing in Improving Photosynthesis in Crop Plants
Bukhat, S., Rasul, S., Athar, H., & Manzoor, H. (2025) · CABI eBooks · doi:10.1079/9781800622517.0016 · Cited by 1
Breeding Strategies for Improved Multistress-Resilient Crops
Lokeshkumar, B. M., Snehi, S., Krishanu, Kumar, S., Ravikiran, K. T., Kumar, R., Choudhary, M., Kota, S., Kumar, A., Mann, A., et al. (2025) · Springer eBooks · doi:10.1007/978-981-96-2508-6_7 · Cited by 1
Directed mutagenesis in fruit crops
Mohanta, R., Maiti, P., Sharangi, A. B., Roy, S., Hazra, S., Chakraborty, S., & Ghorai, S. (2025) · 3 Biotech · doi:10.1007/s13205-025-04268-8 · Cited by 1
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Enhanced pigment production from plants and microbes: a genome editing approach
Harshini, P., Varghese, R., Pachamuthu, K., & Ramamoorthy, S. (2025) · 3 Biotech · doi:10.1007/s13205-025-04290-w · Cited by 1
“However, both ZFN (prevalent since 2002) and TALEN (prevalent since 2011) require prolonged vector construction (Manghwar et al. 2019).”
Influential
Genetic Engineering in Cotton: Techniques and Practices
Esmaeili, N., Hussin, A., Jin, S., Zhang, J., & Zhang, H. (2025) · Compendium of plant genomes · doi:10.1007/978-3-031-84605-2_8 · Cited by 1
Genome editing without the hassles of tissue culture: Hope for editing plants recalcitrant to in vitro manipulations
Vaikuntapu, P. R., Kumar, V. D., Sujatha, M., & RADHAKRISHNAN, T. (2020) · Journal of Oilseeds Research · doi:10.56739/jor.v37i3.136489 · Cited by 1
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Xing, T. (2020) · Journal of Plant Development · doi:10.33628/jpd.2020.27.1.187 · Cited by 1
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Singh, M., Singh, D., Suyal, D. C., Mehra, A., Tripathi, V., Bhasin, S., Rai, P. K., Saxena, R., & Ilyas, A. (2021) · Invertis Journal of Renewable Energy · doi:10.5958/2454-7611.2021.00003.5 · Cited by 1
Characterization of Substitution Mutations of eIF4G Gene Generated through Adenine Base Editors in Rice
Kumam, Y., Rajadurai, C., Kumar, K. K., Varanavasiappan, S., Raveendran, M., Manonmani, S., Gopalakrishnan, C., Arul, L., Kokiladevi, E., & Sudhakar, D. (2021) · Madras Agricultural Journal · doi:10.29321/maj.10.000544 · Cited by 1
“The repair, albeit precise, is error-prone, with no control over the number of nucleotide bases being deleted or inserted, leading to unanticipated mutants (Manghwar et al., 2019; Maruyama et al., 2015; Piergentili et al., 2021).”
Background
CRISPR/Cas for Improved Stress Tolerance in Rice
Islam, K. N., Hasan, M. M., & Islam, M. N. (2022) · Springer eBooks · doi:10.1007/978-981-19-1445-4_12 · Cited by 1
¿Mejoramiento Genético o Manipulación Genética? Aplicaciones de la técnica CRISPR-Cas.
González, E. J. R., Bitzer-Quintero, O. K., & Ramírez-Jirano, L. J. (2022) · Recursos Naturales y Sociedad · doi:10.18846/renaysoc.2022.08.08.02.0001 · Cited by 1
CRISPR/Cas9: An evolutionary approach towards crops amelioration
Kapil, J., Fartyal, M., & Mathur, N. (2023) · LS International Journal of Life Sciences · doi:10.5958/2319-1198.2023.00004.0 · Cited by 1
CRISPR Gen Düzenleme Yöntemi Nedir? CRISPR-Cas9 Sistemini Kullanarak Genleri Nasıl Düzenleriz?Preprint
Özer, O., Karagözoğlu, M., Uysal, Ö., & Akgören, K. (2014) · doi:10.47023/ea.bilim.407 · Cited by 1
The future
Hull, R., Head, G., & Tzotzos, G. T. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818564-3.00010-x · Cited by 1
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Vats, S., Kaur, S., Chauhan, A., Biswas, D. K., & Deshmukh, R. K. (2023) · Wiley eBooks · doi:10.1002/9781394183913.ch4 · Cited by 1
Editorial: Applications of novel gene editing tools and approaches in plants
Cheng, Y., Zhang, T., & Huang, C. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1404959 · Cited by 1
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Yang, S., Liu, H., Hu, J., Chen, B., An, W., Song, X., Yang, Y., & He, F. (2025) · eLife · doi:10.7554/elife.101236 · Cited by 1
“…Church and Jennifer Doudna, uses guide RNAs to target-specific genomic regions, allowing for precise gene removal, insertion, silencing, or activation, with advantages like complete gene deletion despite potential off-target effects ( Lingor, 2010 ; Schuster et al., 2019 ; Manghwar et al., 2019 ).”
A deep learning and co-conservation framework enable discovery of non-canonical Cas proteinsPreprint
He, B., Qi, C., Feng, Y., Liu, D., Wu, R., Wang, Z., Wang, D., Yue, Z., Zhang, Y., Lan, H., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.30.679098 · Cited by 1
Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters
Peng, B., Ma, X., Yang, T., Zheng, M., Peng, J., Tian, X.-Y., YanFang, S., Song, X., Pang, R.-H., Li, J., et al. (2020) · Journal of Biology and Life Science · doi:10.5296/jbls.v11i2.17039
“Abbreviation: Cas9, CRISPR-associated nuclease 9; CRISPR, clustered regularly interspaced short palindromic repeat; HDR, homology-directed repair; PAM, protospacer adjacent motif (Manghwar et al., 2019).”
Background
Genome editing and RNA interference technologies in plants
Tamang, T. M., Kakeshpour, T., Hu, Y., Peng, Z., & Wu, Q. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821120-5.00001-8
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韩, 平. (2021) · Botanical Research · doi:10.12677/br.2021.105088
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Lange, A. (2021) · Springer eBooks · doi:10.1007/978-3-662-63055-6_6
Genoptimierung – vom Traum zur Wirklichkeit?
Lange, A. (2021) · Springer eBooks · doi:10.1007/978-3-662-63055-6_7
Bibliography
(2021) · Elsevier eBooks · doi:10.1016/b978-0-12-817563-7.00029-5
Principles and Practices of Genome Editing in Crop Plants
Yuhong, G., Rasheed, A., Zhuo, Z., Gardiner, J. J., Hassan, M., Fahad, S., Gillani, S. F. A., Batool, M., & Jian, W. (2022) · Springer eBooks · doi:10.1007/978-3-030-96925-7_1 · Cited by 1
PENGGUNAAN CRISPR-CAS SYSTEM SEBAGAI SOLUSI MENGHADAPI RESISTENSI ANTIMIKROBA PADA BAKTERI
Soetedjo, R., Alexander, L., & Tobian, N. (2021) · Essential Essence of Scientific Medical Journal · doi:10.24843/estl.2020.v18.i02.p05
Genome Editing
Stotz, H. U., & Koizuka, N. (2021) · Royal Society of Chemistry eBooks · doi:10.1039/9781788017862-00287
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Tian, Y. (2022) · Advances in Social Science, Education and Humanities Research/Advances in social science, education and humanities research · doi:10.2991/978-2-494069-31-2_289
Biofortification of Oats (Avena sativa)
Rana, M., Priyadarshini, P., Guleria, G., Kaldate, R. C., Kumar, N., Gajghate, R., Dwivedi, K. K., & Ahmed, S. (2023) · Springer eBooks · doi:10.1007/978-981-19-4308-9_11
Genome editing of apple – establishing a workflow for the generation of homohistont mutant lines
Schröpfer, S., & Flachowsky, H. (2023) · Acta Horticulturae · doi:10.17660/actahortic.2023.1362.4
Dimethyladenosine Transferase 1 Homolog Promotes Human Gastric Carcinoma Cell Proliferation and Inhibits Apoptosis via the AKT Pathway
Liu, G., Wang, H., Ran, R., Wang, Y., & Li, Y. (2023) · The Turkish Journal of Gastroenterology · doi:10.5152/tjg.2023.22169
Recombinant DNA technology: revolutionizing regenerative medicine and empowering nanotechnology strategies
Lakshmi, M., Dhanu, A. S., Swamy, G. G., Murugesan, K., Radhakrishnan, A., Murali, U., Jagannathan, S., Elango, B., Mathanmohun, M., & Basalingappa, K. M. (2023) · Recent Research in Science and Technology · doi:10.25081/rrst.2023.15.8732
“…of transgenes or CRISPR components into plants through specialized gene delivery mechanisms 12-20 (Steinberg & Raso, 1998; Wright et al., 2005; Phillips & Tang, 2008; Christian et al., 2010; Butler et al., 2016; Tang et al., 2017; Anzalone et al., 2019; Manghwar et al., 2019; Lin et al., 2020).”
Background
CRISPR-Cas technologies in food security and food revolution
De, B., Singla, R. K., Goswami, T. K., & Shen, B. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-91808-4.00003-1
Construção de modelo in vitro baseados em edição gênica por CRISPR/cas9 no LDLR visando melhor compreensão da influência genética na hipercolesterolemia familial
Mori, A. A. (2023) · doi:10.11606/t.9.2023.tde-12122023-113730
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Nazir, T., Mirza, H. A., & Taha, N. (2024) · Preprints.org · doi:10.20944/preprints202402.0085.v1
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Andreev, D. (2024) · E3S Web of Conferences · doi:10.1051/e3sconf/202448606010
Plant Genome Editing Technologies: An Updated Overview
Agrawal, S., Kumar, P., Das, R., Singh, K., Singh, N., Singh, S., Singh, A. K., Shukla, P. K., Rajput, V. D., Minkina, T., et al. (2024) · Interdisciplinary biotechnological advances · doi:10.1007/978-981-99-9338-3_1
CRISPR-based precision breeding of fruits, vegetables, and ornamental plants
Atia, M. A. M., Salah, A., Elsawah, A., Karam, A., & Abdelmoneim, T. K. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13229-2.00010-7
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Koizuka, N. (2024) · Oleoscience · doi:10.5650/oleoscience.24.141
Additional file 11 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323728
Additional file 4 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323743
Additional file 5 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Wen, L., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323746
Additional file 2 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323737
Additional file 12 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323731
Additional file 7 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323752
Additional file 6 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323749
Additional file 9 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323758
Additional file 2 of Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
Wang, G., Xu, Z., Wang, F., Huang, Y., Xin, Y., Liang, S., Li, B., Si, H., Sun, L., Wang, Q., et al. (2022) · Figshare · doi:10.6084/m9.figshare.19178990
Additional file 8 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323755
Additional file 10 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Wen, L., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323725
Additional file 3 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323740
Additional file 1 of Transcriptomic profiles of non-embryogenic and embryogenic callus cells in a highly regenerative upland cotton line (Gossypium hirsutum L.)
Li, W., Li, W., Parris, S., West, M., Lawson, J., Smathers, M., Li, Z., Jones, D. C., Jin, S., & Saski, C. A. (2020) · TigerPrints (Clemson University) · doi:10.6084/m9.figshare.13323734
Application of Mutagenesis in Food Production and Sustainable Development
Pharmawati, M. (2024) · Sustainable landscape planning and natural resources management · doi:10.1007/978-3-031-50729-8_1
The Progress and Challenges of CRISPR-Cas System from Laboratory to Clinical Practice
Pu, Z., & Cai, X. (2024) · International Journal of Molecular Medical Science · doi:10.5376/ijmms.2024.14.0011
“This genome editing technology can be employed to study the function of genes, such as investigating their role in organisms by knocking out specific genes (Manghwar et al., 2019).”
Attitudes of Algorithms: An Exploration of the Ethics of CRISPR Cas9 through the Lens of ChatGPT
D’Souza, R. F., Mathew, M., & Surapaneni, K. M. (2024) · Indian Journal of Clinical Biochemistry · doi:10.1007/s12291-024-01242-8 · Cited by 1
“Gene editing, facilitated by CRISPR technology, opens the door to modifying these genetic instructions, holding promise for the treatment and prevention of various diseases [4].”
Zebrafish casr affects swim bladder inflation by regulating heart developmentPreprint
Liu, L., Hu, Y.-Y., Xie, B., Zhu, J., Zeng, T., Huang, W., Huang, J. J., Lu, X., Yang, C., Li, J., et al. (2024) · Research Square · doi:10.21203/rs.3.rs-4498455/v1
Genome modifications in cassava
Imran, H., Baig, D. I., Jabbar, W., Gul, A., Gul, W., Öztürk, M., Ünal, B. T., & García‐Caparrós, P. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-26614-0.00023-0 · Cited by 1
Three decades and beyond: Breeding, biotech breakthroughs and future of China's GM insect-resistant cotton
Liang, C., & Jin, S. (2024) · Journal of Integrative Agriculture · doi:10.1016/j.jia.2024.07.041
ACVR2A facilitates trophoblast cell invasion through TCF7/c-JUN pathway in pre-eclampsia progression
Yang, S., Liu, H., Hu, J., Chen, B., An, W., Song, X., Yang, Y., & He, F. (2025) · eLife · doi:10.1101/2024.09.03.24312808
ACVR2A Facilitates Trophoblast Cell Invasion through TCF7/c-JUN Pathway in Pre-eclampsia ProgressionPreprint
Yang, S., Liu, H., Hu, J., Chen, B., An, W., Song, X., Yang, Y., & He, F. (2025) · eLife · doi:10.7554/elife.101236.1
Edición de Genes y CRISPR-Cas: Aplicaciones, Avances y Desafíos: Genes y CRISPR
Elizondo‐Luévano, J. H., Sotelo, L. A. G., Paredes, I. C., de Dios-Romero, V., Villanueva-Terán, C., & Kačániová, M. (2024) · doi:10.29105/agricolis.v1i2.11
ACVR2A Facilitates Trophoblast Cell Invasion through TCF7/c-JUN Pathway in Pre-eclampsia ProgressionPreprint
Yang, S., Liu, H., Hu, J., Chen, B., An, W., Song, X., Yang, Y., & He, F. (2025) · eLife · doi:10.7554/elife.101236.2
Improving Plant Abiotic Stress Tolerance by Modulating LncRNAs Using CRISPR/Cas9 Technology
Waseem, M., Basharat, S., Shaheen, I., Pingwu, L., Shareef, M., & Shahid, N. (2025) · doi:10.1002/9781394280049.ch3
Mechanistic study of the immune defense function of the CRISPR1-Cas system in Enterococcus faecalis
Tao, S., Fang, Y., Zheng, L., Zhang, H., Yao, X., & Liang, W. (2025) · Virulence · doi:10.1080/21505594.2025.2530665
“Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and associated proteins (Cas) constitute an adaptive immune system (CRISPR-Cas) [10] represents the third generation of “genome-targeted editing technology,” following “zinc finger nuclease (ZFN)” [11] and “transcription…”
Gene disruption via a transient hypercompact CRISPR-AsCas12f1 system in Kluyveromyces marxianus
Zhang, K., Wang, D., Hu, S., Li, X., & Hong, J. (2025) · Biotechnology Letters · doi:10.1007/s10529-025-03626-z
“…system has been developed in Kluyveromyces marxianus (Bever et al. 2022; Lee et al. 2018; Li et al. 2021; Lobs et al. 2017; Rajkumar & Morrissey 2022); however, the large size of the Cas9 protein poses challenges for packaging into plasmids and subsequent delivery into cells (Manghwar et al. 2019).”
"Contributions of Crispr and Gene Editing Technologies to Plant Breeding and Biotechnology: Advances, Integrative Approaches, And Future Directions"
Gautam, K. K., & Verma, S. B. V. S. B. (2025) · Journal of research in agriculture and animal science. · doi:10.35629/9459-11072228
Simultaneous detection of cytomegalovirus and Epstein-Barr virus based on RPA-CRISPR-Cas12a/Cas13a system
Zhou, Y., Zhang, K., Wang, X., Ma, C., Fu, S., Wang, Z., & Chen, L. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.115052
Research progress of compact Class 2 Cas effector proteins
Liu, B., Huang, S., & ZHENG, W. (2025) · Scientia Sinica Vitae · doi:10.1360/ssv-2025-0095
The role of sterile plant lines in the management of invasive and allergenic plants in urban environments
Kisvarga, S., Horotán, K., Istvánfi, Z., Táborská, J., Mosonyi, I. D., & Orlóci, L. (2025) · Urban forestry & urban greening · doi:10.1016/j.ufug.2025.129083 · Cited by 1
Integrated Omics and CRISPR Tools for Speed and Precision Genetic Engineering in Crops
Suruthi, G. S., Arun, M., Begum, T. N., Margret, A. A., Rosita, A. S., Robert, A. A., Anand, A. V., & Sampathkumar, P. (2025) · CABI biotechnology series. · doi:10.1079/9781800627307.0012
The Cilia‐Associated Protein CCDC89 Is Dispensable for Male Fertility in Mice
Song, D., Li, Q., Sun, Y., Zhao, H., & Song, T. (2025) · Cytoskeleton · doi:10.1002/cm.70057
“Recently, the CRISPR-Cas9 system has emerged as the most prevalent technique for generating genetically engineered mice (Manghwar et al. 2019; Tyagi et al. 2020; Zhang et al. 2017).”
Role of CRISPR/Cas9/genetically engineered microbial approaches for bioproducts development from lignocellulosic biomass
Tripathi, M., Pathak, S., & Garg, D. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-21604-6.00010-3
Genetic transformation and gene editing in cotton: advances and prospects
Yu, L., Yi, Y., Zhang, X., & Jin, S. (2025) · Scientia Sinica Vitae · doi:10.1360/ssv-2025-0249
Additional file 1 of Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
Wang, G., Xu, Z., Wang, F., Huang, Y., Xin, Y., Liang, S., Li, B., Si, H., Sun, L., Wang, Q., et al. (2022) · Figshare · doi:10.6084/m9.figshare.19178987
Additional file 5 of Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Tuo, D., Yao, Y., Yan, P., Chen, X., Qu, F., Xue, W., Liu, J., Kong, H., Guo, J., Cui, H., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849471
Additional file 3 of Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
Wang, G., Xu, Z., Wang, F., Huang, Y., Xin, Y., Liang, S., Li, B., Si, H., Sun, L., Wang, Q., et al. (2022) · Figshare · doi:10.6084/m9.figshare.19178993
Additional file 1 of Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Ursache, R., Fujita, S., Tendon, V. D., & Geldner, N. (2021) · Figshare · doi:10.6084/m9.figshare.16909731
Additional file 3 of Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Tuo, D., Yao, Y., Yan, P., Chen, X., Qu, F., Xue, W., Liu, J., Kong, H., Guo, J., Cui, H., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849465
Additional file 1 of Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Tuo, D., Yao, Y., Yan, P., Chen, X., Qu, F., Xue, W., Liu, J., Kong, H., Guo, J., Cui, H., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849459
Additional file 2 of Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Tuo, D., Yao, Y., Yan, P., Chen, X., Qu, F., Xue, W., Liu, J., Kong, H., Guo, J., Cui, H., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849462
Additional file 4 of Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
Tuo, D., Yao, Y., Yan, P., Chen, X., Qu, F., Xue, W., Liu, J., Kong, H., Guo, J., Cui, H., et al. (2023) · Figshare · doi:10.6084/m9.figshare.23849468
Additional file 3 of Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Ursache, R., Fujita, S., Tendon, V. D., & Geldner, N. (2021) · Figshare · doi:10.6084/m9.figshare.16909737
Additional file 2 of Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Ursache, R., Fujita, S., Tendon, V. D., & Geldner, N. (2021) · Figshare · doi:10.6084/m9.figshare.16909734
Liposome-Based Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein Systems: Advances in Targeted Delivery and Theranostics
Kim, Y., Kim, G.-J., & Kwon, O. S. (2025) · Applied Science and Convergence Technology · doi:10.5757/asct.2025.34.5.148

Authors: Manghwar, H., Lindsey, K., Zhang, X., & Jin, S.
Affiliations: Huazhong Agricultural University (CN)
Durham University (GB)
Corresponding author: Zhang, Xianlong
Jin, Shuangxia
ORCID: Manghwar, Hakim
Lindsey, Keith
Zhang, Xianlong
Jin, Shuangxia
Venue: Trends in Plant Science
Publisher: Elsevier BV
Published: 2019-12
Volume: 24
Issue: 12
Pages: 1102-1125
ISSN: 1360-1385 (ISSN-L)
DOI: 10.1016/j.tplants.2019.09.006
OpenAlex: W2984281074
Cited by: 529
References: 140
Citations by year: 2026: 49 · 2025: 84 · 2024: 94 · 2023: 91 · 2022: 76 · 2021: 91 · 2020: 43
FWCI: 4.65
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-nd
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by-nc-nd/4.0/ (vor)
Locations: Trends in Plant Science (publishedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
Durham Research Online (Durham University) (publishedVersion, other-oa)
Durham Research Online (Durham University) (acceptedVersion, other-oa)
Durham Research Online (Durham University) (submittedVersion, cc-by-nc-nd)
Funders: Ministry of Science and Technology of the People's Republic of China
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords: CRISPR/Cas9, genome editing, base editing, genome editing tools
MeSH terms: Gene Editing, Crops, Agricultural, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems

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CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

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