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The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, Bernd, Gootenberg, Jonathan S., Abudayyeh, Omar O., Slaymaker, Ian M., Makarova, Kira S., Essletzbichler, Patrick, Volz, Sara E., Joung, Julia, van der Oost, John, Regev, Aviv, Koonin, Eugene V., Zhang, Feng·2015·Cell
DOI 10.1016/j.cell.2015.09.038PMC PMC4638220
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  • Cas12a produces staggered cuts with 5-nt 5′-overhangs, whereas Cas9 produces blunt cuts.

    “the resulting DSBs are staggered cuts with 5-nt 5′-overhangs as opposed to the blunt cuts generated by Cas9 [116].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [116]

    The Results section reports that FnCpf1 makes staggered cuts with 5-nt 5′ overhangs, unlike the blunt cuts made by Cas9. It also reports staggered cuts for AsCpf1 and LbCpf1, but does not establish a 5-nt overhang for those proteins or Cas12a generally. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Cas12a differs from Cas9 by self-catalysis of crRNA maturation.

    “their distinguishable differences from Cas9 include: a T nucleotide-rich PAM site (5′-TTTV), self-catalysis of crRNA maturation, and a staggered dsDNA cut [76,77,78,79].”

    From Viral Delivery Systems for CRISPR (2019) · cites this paper as [76]

    The Results section, “The Cpf1-associated CRISPR array is processed independent of tracrRNA” (page unavailable), reports crRNA maturation when FnCpf1 and its CRISPR array were the only elements supplied from that locus. The Discussion contrasts this tracrRNA-independent processing with Cas9, but says the processing mechanism still needs to be elucidated; it does not establish that Cpf1 itself catalyzes maturation. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Cas12a enzymes are single crRNA-guided endonucleases measuring 3.9 kb.

    “Cas12a enzymes are single crRNA-guided endonucleases measuring 3.9 kb”

    From Viral Delivery Systems for CRISPR (2019) · cites this paper as [76]

    The Discussion identifies Cpf1 as a single crRNA-guided endonuclease. The Results section on Cpf1-family proteins gives a range of approximately 1,200–1,500 amino acids, but our copy does not state that the enzymes measure 3.9 kb; that specific size is unsupported. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Cas12a requires only a crRNA molecule to guide it, unlike Cas9 which requires a crRNA:tracrRNA dual guide.

    “However, in contrast to Cas9, it requires only a crRNA molecule to guide it towards its target, in contrast with the crRNA:tracrRNA dual guide of Cas9”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [116]

  • Cas12a differs from Cas9 by a staggered dsDNA cut.

    “their distinguishable differences from Cas9 include: a T nucleotide-rich PAM site (5′-TTTV), self-catalysis of crRNA maturation, and a staggered dsDNA cut [76,77,78,79].”

    From Viral Delivery Systems for CRISPR (2019) · cites this paper as [76]

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Citations

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Research works printed but not linked: 36

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Metadata

Authors
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., Koonin, E. V., & Zhang, F.
Venue
Cell
Publisher
Elsevier BV
Published
2015-10
Volume
163
Issue
3
Pages
759-771
ISSN
0092-8674
DOI
10.1016/j.cell.2015.09.038
PMC
PMC4638220
Cited by
4,761
References
12
Language
en
Licence

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