CoChat
Sign Up
Cited by 65Mosquito-borne diseases and controlViral Infections and VectorsDengue and Mosquito Control Research

RNA-Seq analysis of peripheral blood mononuclear cells reveals unique transcriptional signatures associated with disease progression in dengue patients

Banerjee, Arup, Shukla, Shweta, Pandey, Abhay Deep, Goswami, Saptamita, Bandyopadhyay, Bhaswati, Ramachandran, Vishnampettai, Das, Shukla, Malhotra, Arjun +9 more·2017·Translational Research
DOI 10.1016/j.trsl.2017.06.007
Open this paper in CoChat — free account

Abstract

Patients infected with Dengue virus usually present a mild, self-limiting febrile dengue infection (DI) that occasionally leads to a potentially lethal complication, called the severe dengue (DS). The ability to identify the prognostic markers of DS could allow an improved disease intervention and management. To identify the transcriptional signatures associated with the dengue disease progression, we carried out the high-throughput sequencing of the RNA isolated from the peripheral blood mononuclear cells (PBMCs) of the dengue patients of varying severity and compared with that in the patients with other febrile illnesses (OFIs) or the healthy controls. The transcriptional signatures that discriminated the DS patients from OFI and DI patients were broadly related to the pathways involving glycine, serine, and threonine metabolisms, extracellular matrix organization, ubiquitination, and cytokines and inflammatory response. Several upregulated genes in the inflammatory process (MPO, DEFA4, ELANE, AUZ1, CTSG, OLFM4, SLC16A14, and CRISP3) that were associated with the dengue disease progression are known to facilitate leukocyte-mediated migration, and neutrophil activation and degranulation process. High activity of MPO and ELANE in the plasma samples of the follow-up and recovered dengue patients, as well as and the presence of a larger amount of cell-free dsDNA in the DS patients, suggested an association of neutrophil-mediated immunity with dengue disease progression. Careful monitoring of some of these gene transcripts, and control of the activity of proteins encoded by them, may have a great translational significance for the prognosis and management of the dengue patients.

Integrity signals

Registry

Crossref · 10.1016/j.trsl.2017.06.007

Cites

41 of 41 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • RNA-seq of the human host can reveal new or underappreciated host-microbial interactions directly from clinical samples, as shown in Lyme disease, dengue, and malaria.

    “Moreover, RNA-seq analyses of the human host can be used to identify novel or underappreciated host–microbial interactions directly from clinical samples, as previously shown for patients with Lyme disease68, dengue80 or malaria81.”

    From Clinical metagenomics (2019) · cites this paper as [80]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Banerjee, A., Shukla, S., Pandey, A. D., Goswami, S., Bandyopadhyay, B., Ramachandran, V., Das, S., Malhotra, A., Agarwal, A., Adhikari, S., Rahman, M., Chatterjee, S., Bhattacharya, N., Basu, N., Pandey, P., Sood, V., & Vrati, S.
Affiliations
  • Translational Health Science and Technology Institute (IN)
  • University College of Medical Sciences (IN)
  • School of Tropical Medicine (IN)
  • National Institute of Biomedical Genomics (IN)
Corresponding author
  • Banerjee, Arup
  • Vrati, Sudhanshu
ORCID
Venue
Translational Research
Publisher
Elsevier BV
Published
2017-08
Volume
186
Pages
62-78.e9
ISSN
  • 1878-1810 (ISSN-L)
  • 1931-5244
DOI
10.1016/j.trsl.2017.06.007
OpenAlex
W2638117886
Cited by
65
References
41
Citations by year
2026: 13 · 2025: 8 · 2024: 7 · 2023: 13 · 2022: 9 · 2021: 5 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 1
FWCI
0.44
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Translational research (publishedVersion)
  • PubMed (publishedVersion)
Funders
Department of Biotechnology (DBT), Government of India (BT/PR8597/MED/29/764/2013)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
  • Social Sciences › Sociology and Political Science › Dengue and Mosquito Control Research
Keywords
dengue, severe dengue, disease progression, peripheral blood mononuclear cells, gene expression signatures, prognostic factors, neutrophil activation, neutrophil degranulation, myeloperoxidase, neutrophil elastase, cell-free DNA, inflammation, cytokines, glycine, serine and threonine metabolism, extracellular matrix organization, ubiquitination, febrile illness
MeSH terms
Adolescent, Adult, Base Sequence, Child, Dengue, Dengue Virus, Female, Gene Expression Regulation, Humans, Leukocytes, Mononuclear, Male, RNA, Transcription, Genetic, Young Adult, Transcriptome

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review