Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology
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The objective of this study was to investigate the effect of key extraction parameters of extraction time (5-25 min), acid concentration (0-0.06 M HCl) and ultrasound amplitude (22.8-114 μm) on yields of bioactive compounds (total phenolics, fucose and uronic acid) from Ascophyllumnodosum. Response surface methodology was employed to optimize the extraction variables for bioactive compounds' yield. A second order polynomial model was fitted well to the extraction experimental data with (R(2)>0.79). Extraction yields of 143.12 mgGAE/gdb, 87.06 mg/gdb and 128.54 mg/gdb were obtained for total phenolics, fucose and uronic acid respectively at optimized extraction conditions of extraction time (25 min), acid concentration (0.03 M HCl) and ultrasonic amplitude (114 μm). Mass spectroscopy analysis of extracts show that ultrasound enhances the extraction of high molecular weight phenolic compounds from A. nodosum. This study demonstrates that ultrasound assisted extraction (UAE) can be employed to enhance extraction of bioactive compounds from seaweed.
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Kadam et al. observed extraction of fucose and uronic acid; higher extraction yield for phenolic content and uronic acid when acid used, while acid did not contribute to higher fucose content; higher acid concentration decreased extraction yield due to acid hydrolysis and degradation.
“Except the extraction of phenols, Kadam et al. [52] also observed the extraction of fucose and uronic acid. They showed that the higher extraction yield was obtained for phenolic content and uronic acid when acid was used, while the use of acid did not contribute to the higher fucose content. If the higher concentration of acid was used, the decrease in the extraction yield occurred due to acid”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [52]
Higher amount of TPC is shown by Kadam et al. because they used acid (0.03 M HCl) as solvent, while Dang et al. used ethanol; both authors used various species, which explained difference.
“The higher amount of TPC is shown by Kadam et al. [52], because they used acid (0.03 M HCl) as solvent, while Dang et al. [49] used ethanol. But, both authors used various species of macroalgae, which explained the difference between amounts of extracted polyphenols.”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [52]
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References 32 · Cited by 220
- Authors
- Kadam, S. U., Tiwari, B. K., Smyth, T. J., & O’Donnell, C. P.
- Affiliations
- University College Dublin (IE)
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Corresponding author
- Tiwari, Brijesh K.
- Venue
- Ultrasonics Sonochemistry
- Publisher
- Elsevier BV
- Published
- 2015-03
- Volume
- 23
- Pages
- 308-316
- ISSN
- 1350-4177 (ISSN-L)
- OpenAlex
- W2065334338
- Cited by
- 220
- References
- 32
- Citations by year
- 2026: 20 · 2025: 24 · 2024: 20 · 2023: 12 · 2022: 32 · 2021: 21 · 2020: 25 · 2019: 25 · 2018: 14 · 2017: 13 · 2016: 5 · 2015: 9
- FWCI
- 13.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Ultrasonics Sonochemistry (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Irish Research Council’s Embark Postgraduate Research Scholarship Scheme
- Marine Functional Foods Research Initiative (MFFRI/07/01)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Physical Sciences › Materials Science › Materials Chemistry › Ultrasound and Cavitation Phenomena
- Keywords
- ultrasound-assisted extraction, Ascophyllum nodosum, brown algae, bioactive compounds, total phenolic content, fucose, uronic acid, extraction optimization, extraction time, acid concentration, ultrasonic amplitude
- MeSH terms
- Chemical Fractionation, Fucose, Hydrochloric Acid, Models, Theoretical, Phenols, Seaweed, Statistics as Topic, Tannins, Time Factors, Ultrasonics, Uronic Acids, Ascophyllum
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