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Cited by 22Microbial infections and disease researchAnimal health and immunologyEscherichia coli research studies

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IbpA DR2 subunit immunization protects calves against Histophilus somni pneumonia

Geertsema, R.S., Zekarias, B., Scheuch, L. La Franco, Worby, C., Russo, R., Gershwin, L.J., Herdman, D.S., Lo, K., Corbeil, L.B.·2011·Vaccine
DOI 10.1016/j.vaccine.2011.04.075
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Abstract

Histophilus somni is a prevalent cause of pneumonia and septicemia in cattle. Yet evidence for protection against pneumonia by current vaccines is controversial. We have identified a new H. somni virulence factor, IbpA. Previous studies implicated three likely protective subunits or domains in IbpA (A3, A5, and DR2), which were expressed as recombinant GST fusion proteins and purified for systemic vaccination of calves. After two subcutaneous immunizations, calves were challenged intrabronchially with virulent H. somni strain 2336 and clinical signs were monitored for four days before necropsy. Serum samples were collected throughout. At necropsy, the area of gross pneumonia was estimated, bronchial lavage fluid was collected, lesions were cultured and tissue samples were fixed for histopathology. Results showed that calves immunized with IbpA DR2 had a statistically lower percentage of lung with gross lesions than controls, fewer histologic abnormalities in affected areas and no H. somni isolated from residual pneumonic lesions. Calves immunized with the control GST vaccine, IbpA3 or IbpA5 had larger H. somni positive pneumonic lesions. ELISA results for serum antibodies showed that calves immunized with the IbpA DR2 antigen had high IgG1 and IgG2 and lowest IgE responses to the immunizing antigen. Specific IgG responses were also high in the bronchial lavage fluid. High specific serum IgE responses were previously shown to be associated with more severe pneumonia, but high…

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Research works printed but not linked: 33

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Metadata

Authors
Geertsema, R. S., Zekarias, B., Scheuch, L. L. F., Worby, C., Russo, R., Gershwin, L. J., Herdman, D. S., Lo, K., & Corbeil, L. B.
Affiliations
  • University of California San Diego (US)
  • University of California, Davis (US)
Corresponding author
Corbeil, L.B.
ORCID
Venue
Vaccine
Publisher
Elsevier BV
Published
2011-06
Volume
29
Issue
29-30
Pages
4805-4812
ISSN
0264-410X (ISSN-L)
DOI
10.1016/j.vaccine.2011.04.075
OpenAlex
W1995701131
Cited by
22
References
0
Citations by year
2026: 2 · 2024: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 3 · 2013: 2 · 2012: 3
FWCI
1.77
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Vaccine (publishedVersion)
  • PubMed (publishedVersion)
Funders
USDA National Research (2005-35204-6257)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal health and immunology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Keywords
Histophilus somni, IbpA, bovine pneumonia, protective antigen, subunit vaccine, calves, antibody neutralization, IgG antibody response, IgE, virulence factors
MeSH terms
Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Vaccines, Bronchoalveolar Lavage Fluid, Cattle, Enzyme-Linked Immunosorbent Assay, Histocytochemistry, Immunoglobulin E, Immunoglobulin G, Immunization, Secondary, Injections, Subcutaneous, Lung, Recombinant Proteins, Severity of Illness Index, Vaccination, Vaccines, Synthetic, Pasteurellaceae, Pasteurellaceae Infections, Pneumonia, Bacterial, Protein Subunits, Virulence Factors

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CoChat. (2026). Citation check: IbpA DR2 subunit immunization protects calves against Histophilus somni pneumonia. https://app.cochat.ai/papers/10.1016/j.vaccine.2011.04.075
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Geertsema, R. S., Zekarias, B., Scheuch, L. L. F., Worby, C., Russo, R., Gershwin, L. J., Herdman, D. S., Lo, K., & Corbeil, L. B. (2011). IbpA DR2 subunit immunization protects calves against Histophilus somni pneumonia. Vaccine. https://doi.org/10.1016/j.vaccine.2011.04.075