An mRNA-based vaccine candidate against SARS-CoV-2 elicits stable immuno-response with single dose
Open accessAbstract
D614G genotype of SARS-CoV-2 virus is highly infectious and responsible for almost all infection for 2nd wave. However, there are currently no reports with D614G as vaccine candidate. Here we report the development of an mRNA-LNP vaccine with D614G variant and characterization in animal model. We have used special mRNA-architecture and formulation that provides suitable response of the product. The surface plasmon resonance (SPR) data with spike protein (S) revealed that immunization generated specific antibody pools against the whole extracellular domain (RBD and S2) of the spike protein. The anti-sera and purified IgGs from immunized mice neutralized SARS-CoV-2-pseudoviruses in ACE2-expressing HEK293 cells in a dose dependent manner. Importantly, single-dose immunization protected mice-lungs from homotypic-pseudovirus entry and cytopathy. The immunologic responses have been implicated by a balanced and stable population of CD4+ cells with a Th1 bias. The data suggested great promise for immediate translation of the technology to the clinic.
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- Authors
- Nag, K., Chandra Baray, J., Rahman Khan, M., Mahmud, A., Islam, J., Myti, S., Ali, R., Haq Sarker, E., Kumar, S., Hossain Chowdhury, M., Roy, R., Islam, F., Barman, U., Khan, H., Chakraborty, S., Badsha, A., Hossain, M., Ahammad, S., Rahman Chowdhury, M., Ghosh, P., Islam Shimul, R., Ahmmed, R., Hussain Bhuiya, E., Kumar Biswas, B., Mohiuddin, M., & Sultana, N.
- Corresponding author
- Nag, Kakon
- Venue
- Vaccine
- Publisher
- Elsevier BV
- Published
- 2021-06
- Volume
- 39
- Issue
- 28
- Pages
- 3745-3755
- ISSN
- 0264-410X (ISSN-L)
- PMC
- PMC8130517
- OpenAlex
- W3161853215
- Cited by
- 13
- References
- 47
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1 · 2022: 7 · 2021: 3
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Vaccine (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
- Keywords
- SARS-CoV-2, D614G mutation, mRNA vaccines, lipid nanoparticle formulation, spike protein, receptor binding domain, S2 domain, neutralizing antibodies, pseudovirus neutralization, single-dose vaccination, lung protection, CD4+ T cells, Th1 response, animal models
- MeSH terms
- COVID-19, SARS-CoV-2, Animals, Antibodies, Viral, Humans, RNA, Messenger, Vaccines, Mice, HEK293 Cells, Spike Glycoprotein, Coronavirus
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