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Cited by 12Vector-borne infectious diseasesBrucella: diagnosis, epidemiology, treatmentMicrobial infections and disease research

The DOI matches its Crossref record; 9 of the 10 references it cites were found in a registry or an index.

Demonstration of the ability of a canine Lyme vaccine to reduce the incidence of histological synovial lesions following experimentally-induced canine Lyme borreliosis

Grosenbaugh, Deborah A., Rissi, Daniel R., Krimer, Paula M.·2016·Veterinary Immunology and Immunopathology
DOI 10.1016/j.vetimm.2016.08.014
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Abstract

Lyme disease in dogs can be effectively prevented by vaccination against antigens expressed by the spirochete Borrelia burgdorferi during transmission by the tick vector Ixodes sp. Lyme vaccine efficacy has traditionally been based on indicators of infection following wild-caught tick challenge whereas most other types of vaccine are required to demonstrate protection from clinical signs of disease. In this vaccination-challenge study we sought to demonstrate the ability of a nonadjuvanted, outer surface protein A (OspA) vaccine to protect from infection and to prevent synovial lesions consistent with Borreliosis. Thirty, purpose-bred beagles were randomly divided into vaccinated and unvaccinated groups. The vaccinated group was administered two subcutaneous doses of a nonadjuvanted, purified, Borrelia burgdorferi OspA vaccine at a 21- day interval. Dogs were challenged by wild-caught, B. burgdorferi-infected ticks (Ixodes scapularis). Clinical signs, serology, Borrelia isolation and PCR evaluated antemortem vaccine efficacy. Postmortem histopathological analysis of synovial tissue was compared to antemortem infection status. Borreliosis was demonstrated by Borrelia isolation from skin biopsies in 13 out of 15 unvaccinated dogs. All unvaccinated dogs' Western blot profiles were consistent with infection. Two of 15 vaccinated dogs had at least one positive spirochete culture which cleared 91days post-challenge, and Western blot profiles were consistent with vaccination alone. No dogs, vaccinated or unvaccinated, exhibited clinical signs consistent with borreliosis. Based on a histopathological cumulative joint scoring system (CJS), all unvaccinated dogs had synovial lesions indicative of Lyme disease. Only one of the vaccinated dogs had a CJS that was greater than the statistical cut off score for the absence of synovial lesions. There was high correlation between clinical histopathology and spirochete isolation. Infection with B burgdorferi may produce inconsistent clinical signs of lameness. Histopathological changes in joints from infected dogs are reliable indicators of borreliosis and correlate well with other indicators of infection. This model provides support that vaccination with a nonadjuvanted, purified OspA vaccine offers protection from Borrelia infection and the resulting synovial lesions that can lead to clinical signs of lameness.

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Crossref · 10.1016/j.vetimm.2016.08.014

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Metadata

Authors
Grosenbaugh, D. A., Rissi, D. R., & Krimer, P. M.
Affiliations
University of Georgia (US)
Corresponding author
Grosenbaugh, Deborah A.
ORCID
Venue
Veterinary Immunology and Immunopathology
Publisher
Elsevier BV
Published
2016-11
Volume
180
Pages
29-33
ISSN
0165-2427 (ISSN-L)
DOI
10.1016/j.vetimm.2016.08.014
OpenAlex
W2511026750
Cited by
12
References
10
Citations by year
2026: 1 · 2022: 4 · 2021: 2 · 2018: 4 · 2017: 1
FWCI
1.72
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Immunology and Immunopathology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Merial, Inc
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
  • Health Sciences › Medicine › Infectious Diseases › Brucella: diagnosis, epidemiology, treatment
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Borrelia burgdorferi, vaccine efficacy, synovial lesions, Ixodes scapularis, tick challenge, joint histopathology, lameness, serology, spirochete isolation, beagle dogs, clinical signs of infection
MeSH terms
Animals, Antigens, Surface, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Dog Diseases, Dogs, Female, Lameness, Animal, Lipoproteins, Lyme Disease, Male, Synovial Membrane, Vaccination, Borrelia burgdorferi

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