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Cited by 73Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchImmunotherapy and Immune Responses

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Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii

Hiszczyńska-Sawicka, Elżbieta, Olędzka, Gabriela, Holec-Gąsior, Lucyna, Li, Hong, Xu, Janet Boyu, Sedcole, Richard, Kur, Józef, Bickerstaffe, Roy, Stankiewicz, Mirosław·2011·Veterinary Parasitology
DOI 10.1016/j.vetpar.2010.11.047
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Abstract

The dense granule proteins of Toxoplasma gondii are investigated as possible vaccine candidates against the parasite. The aim of this research was to evaluate the immune responses of sheep injected twice, intramuscularly, with DNA plasmids encoding T. gondii dense granule antigens GRA1, GRA4, GRA6 and GRA7 formulated into liposomes. Control sheep were injected with an empty vector or received no injections. The injection of sheep with DNA plasmids encoding for GRA1, GRA4, GRA6 or GRA7 elicited an immune response after the first and the second injections as indicated by the moderate to high antibody responses. The injection of pGRA7 induced a significant level of anti-GRA7 IgG2 antibody and IFN-γ responses indicating a Th1-like immune response whereas injection with pGRA1, pGRA4 and pGRA6 stimulated a IgG1 type antibody response with a limited, if any, IFN-γ response. The results demonstrate that the intramuscular injection of sheep with a DNA liposome formulated plasmid coding for GRA proteins is an effective system that induces a significant immune response against T. gondii.

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Metadata

Authors
Hiszczyńska-Sawicka, E., Olędzka, G., Holec-Gąsior, L., Li, H., Xu, J. B., Sedcole, R., Kur, J., Bickerstaffe, R., & Stankiewicz, M.
Affiliations
  • Lincoln University (NZ)
  • Medical University of Warsaw (PL)
  • Gdańsk University of Technology (PL)
Corresponding author
Hiszczyńska-Sawicka, Elżbieta
ORCID
Venue
Veterinary Parasitology
Publisher
Elsevier BV
Published
2011-05
Volume
177
Issue
3-4
Pages
281-289
ISSN
0304-4017 (ISSN-L)
DOI
10.1016/j.vetpar.2010.11.047
OpenAlex
W2083132742
Cited by
73
References
0
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 7 · 2019: 9 · 2018: 5 · 2017: 5 · 2016: 7 · 2015: 2 · 2014: 11 · 2013: 6 · 2012: 8
FWCI
3.94
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Veterinary Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
Toxoplasma gondii, GRA1, GRA6, GRA7, DNA vaccine, sheep, vaccine candidate, antibody response, interferon-gamma, Th1 response, IgG1, IgG2
MeSH terms
Animals, Antibodies, Protozoan, Enzyme-Linked Immunosorbent Assay, Female, Immunization, Interferon-gamma, Liposomes, Plasmids, Random Allocation, Sheep, Sheep Diseases, Toxoplasma, Toxoplasmosis, Animal, Protozoan Proteins, Protozoan Vaccines, DNA, Protozoan, Vaccines, DNA

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CoChat. (2026). Citation check: Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii. https://app.cochat.ai/papers/10.1016/j.vetpar.2010.11.047
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Hiszczyńska-Sawicka, E., Olędzka, G., Holec-Gąsior, L., Li, H., Xu, J. B., Sedcole, R., Kur, J., Bickerstaffe, R., & Stankiewicz, M. (2011). Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii. Veterinary Parasitology. https://doi.org/10.1016/j.vetpar.2010.11.047