AGS and other tissue culture cells can unknowingly be persistently infected with PIV5; A virus that blocks interferon signalling by degrading STAT1
Open accessAbstract
Whilst screening various cell lines for their ability to respond to interferon (IFN), we noted that in comparison to other tissue culture cells AGS tumour cells, which are widely used in biomedical research, had very low levels of STAT1. Subsequent analysis showed that the reason for this is that AGS cells are persistently infected with parainfluenza virus type 5 (PIV5; formally known as SV5), a virus that blocks the interferon (IFN) response by targeting STAT1 for proteasome-mediated degradation. Virus protein expression in AGS is altered in comparison to the normal pattern of virus protein synthesis observed in acutely infected cells, suggesting that the AGS virus is defective. We discuss the relevance of these results in terms of the need to screen cell lines for persistent virus infections that can alter cellular functions.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Respiratory viruses are able to induce ciliary impairment, including disruption of ciliary beating and loss of ciliated cells, leading to mucostasis.
“Respiratory viruses are also able to induce ciliary impairment, including disruption of ciliary beating and loss of ciliated cells, leading to mucostasis (318, 332).”
From The airway epithelium: soldier in the fight against respiratory viruses (2011) · cites this paper as [332]
Cites
Andrejeva, J., Childs, K., Young, D. F., Carlos, T. S., Stock, N., Goodbourn, S., & Randall, R. E. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407639101 · Cited by 990 · see citing works
Self-citationDidcock, L., Young, D. F., Goodbourn, S., & Randall, R. E. (1999) · Journal of Virology · doi:10.1128/jvi.73.12.9928-9933.1999 · Cited by 422 · see citing works
Self-citationmda-5, but not RIG-I, is a common target for paramyxovirus V proteins
Childs, K., Stock, N., Ross, C., Andrejeva, J., Hilton, L. S., Skinner, M. A., Randall, R. E., & Goodbourn, S. (2006) · Virology · doi:10.1016/j.virol.2006.09.023 · Cited by 299 · see citing works
Self-citationBARRANCO, S. C., Townsend, C. M., Casartelli, C., Macik, B. G., Burger, N. L., Boerwinkle, W. R., & Gourley, W. K. (1983) · PubMed · Cited by 232 · see citing works
Horvath, C. M. (2004) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.2004.04425.x · Cited by 161 · see citing works
Randall, R. E., Young, D. F., Goswami, K. K. A., et al. (1987) · Journal of General Virology · doi:10.1099/0022-1317-68-11-2769 · Cited by 161 · see citing works
Self-citationChatziandreou, N., Stock, N., Young, D. F., Andrejeva, J., Hagmaier, K., McGeoch, D. J., & Randall, R. E. (2004) · Journal of General Virology · doi:10.1099/vir.0.80200-0 · Cited by 83 · see citing works
Self-citationAbril, E., Real, L. M., Serrano, A., et al. (1998) · Cancer Immunology Immunotherapy · doi:10.1007/s002620050511 · Cited by 66 · see citing works
Chatziandreou, N., Young, D. F., Andrejeva, J., Goodbourn, S., & Randall, R. E. (2002) · Virology · doi:10.1006/viro.2001.1302 · Cited by 59 · see citing works
Self-citationParainfluenza-5 virus. Infection of man and animal.
Gd, H. (1972) · PubMed · Cited by 59 · see citing works
DNA fingerprinting transforms the art of cell authentication
Stacey, G. N., Bolton, B. J., & Doyle, A. (1992) · Nature · doi:10.1038/357261a0 · Cited by 55 · see citing works
Abril, E., Mendez, R. E., Garcia-Lora, A. M., Serrano, A., Cabrera, T., Garrido, F., & Ruiz‐Cabello, F. (1996) · Tissue Antigens · doi:10.1111/j.1399-0039.1996.tb02574.x · Cited by 55 · see citing works
Mitchell, D. J., Huynh, H. Q., Ceponis, P. J. M., Jones, N. L., & Sherman, P. M. (2003) · Infection and Immunity · doi:10.1128/iai.72.1.537-545.2004 · Cited by 36 · see citing works
Modulation of Host Gene Expression and Innate Immunity by Viruses
Johnston, J. B., & McFadden, G. (2005) · Springer eBooks · doi:10.1007/1-4020-3242-0 · Cited by 35 · see citing works
Carlos, T. S., Fearns, R., & Randall, R. E. (2005) · Journal of Virology · doi:10.1128/jvi.79.22.14112-14121.2005 · Cited by 33 · see citing works
Self-citationHeterogeneous responses of an in vitro model of human stomach cancer to anticancer drugs
BARRANCO, S. C., Townsend, C. M., Quraishi, M. A., et al. (1983) · Investigational New Drugs · doi:10.1007/bf00172070 · Cited by 31 · see citing works
The Anti-Interferon Mechanisms of Paramyxoviruses
Stock, N., Goodbourn, S., & Randall, R. E. (2006) · Springer eBooks · doi:10.1007/1-4020-3242-0_6 · Cited by 11 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
The airway epithelium: soldier in the fight against respiratory viruses
Vareille, M., Kieninger, E., Edwards, M. R., & Regamey, N. (2011) · Clinical Microbiology Reviews · doi:10.1128/cmr.00014-10 · Cited by 655 · see citing works
- Authors
- Young, D. F., Carlos, T. S., Hagmaier, K., Fan, L., & Randall, R. E.
- Affiliations
- University of St Andrews (GB)
- Corresponding author
- Randall, R.E.
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2007-08
- Volume
- 365
- Issue
- 1
- Pages
- 238-240
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2076174740
- Cited by
- 28
- References
- 17
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 1 · 2014: 2 · 2012: 1
- FWCI
- 0.74
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- viral persistence, AGS cells, interferon signaling, STAT1 degradation, proteasomal degradation, defective virus, cell line screening, cancer cell lines
- MeSH terms
- Cell Line, Cells, Cultured, Interferons, Paramyxoviridae, Signal Transduction, Viral Structural Proteins, STAT1 Transcription Factor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
