The DOI matches its Crossref record; 42 of the 43 references it cites were found in a registry or an index.
Nucleocapsid protein of SARS-CoV activates interleukin-6 expression through cellular transcription factor NF-κB
Open accessAbstract
High levels of interleukin-6 (IL-6) in the acute stage associated with lung lesions were found in SARS patients. To evaluate the mechanisms behind this event, we investigated the roles of SARS-CoV proteins in the regulation of IL-6. Results showed that the viral nucleocapsid (N) protein activated IL-6 expression in a concentration-dependent manner. Promoter analyses suggested that NF-kappaB binding element was required for IL-6 expression regulated by N protein. Further studies demonstrated that N protein bound directly to NF-kappaB element on the promoter. We also showed that N protein activated IL-6 expression through NF-kappaB by facilitating the translocation of NF-kappaB from cytosol to nucleus. Mutational analyses revealed that two regions of N protein were essential for its function in the activation of IL-6. These results provided new insights into understanding the mechanism involved in the function of SARS-CoV N protein and pathogenesis of the virus.
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“In addition to this PRR-mediated activation of NF-κB, the nucleocapsid protein of SARS-CoV-1 activates IL-6 expression through NF-κB by facilitating the translocation of NF-κB from the cytosol to the nucleus (341).”
From IL-6 in inflammation, autoimmunity and cancer (2021) · cites this paper as [341]
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2 retracted
Yan, X., Qian, H., Mu, Y., Timani, K. A., Ye, L., Zhu, Y., & Wu, J. (2006) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2006.02.003 · Cited by 134 · cited 2×
“Our recent results indicated that N protein activates COX-2 gene expression by binding either directly or indirectly to NF-κB and C/EBP regulatory elements on COX-2 promoter (Yan et al., 2006).”
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“The N protein of SARS-CoV is 422 amino acids long, sharing only 20–30% homology with the N proteins of other coronaviruses (Marra et al., 2003; Rota et al., 2003).”
BackgroundSelf-citationThe Genome Sequence of the SARS-Associated Coronavirus
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“The N protein of SARS-CoV is 422 amino acids long, sharing only 20-30% homology with the N proteins of other coronaviruses (Marra et al., 2003; Rota et al., 2003) .”
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Items 101–141 of 141
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“Zhang et al. [51] Zhang et al. [51] previously reported that the expression of the carboxyl terminal domain (CTD) of the N protein alone is sufficient for inducing IL-6. previously reported that the expression of the carboxyl terminal domain (CTD) of the N protein alone sufficient for inducing IL-6.”
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Metadata
- Authors
- Zhang, X., Wu, K., Wang, D., Yue, X., Song, D., Zhu, Y., & Wu, J.
- Affiliations
- Wuhan University (CN)
- Guangxi Normal University (CN)
- Corresponding author
- Wu, Kailang
- Zhu, Ying
- Wu, Jianguo
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2007-09
- Volume
- 365
- Issue
- 2
- Pages
- 324-335
- ISSN
- 0042-6822 (ISSN-L)
- PMC
- PMC7103332
- OpenAlex
- W2001975441
- Cited by
- 141
- References
- 43
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 4 · 2023: 12 · 2022: 19 · 2021: 26 · 2020: 43 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 4 · 2012: 1
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30470087)
- Major State Basic Research Development Program of China (2005CB522901)
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
- Health Sciences › Medicine › Infectious Diseases › COVID-19 Clinical Research Studies
- Keywords
- SARS-CoV, nucleocapsid protein, interleukin-6, NF-κB, transcriptional activation, promoter regulation, NF-κB nuclear translocation, viral pathogenesis, SARS
- MeSH terms
- Coronavirus Nucleocapsid Proteins, Cell Line, Cell Nucleus, Cytoplasm, Epithelial Cells, Humans, Luciferases, Lung, Mutation, Protein Binding, Interleukin-6, NF-kappa B, Sequence Deletion, Genes, Reporter, Nucleocapsid Proteins, Artificial Gene Fusion, Protein Transport, Electrophoretic Mobility Shift Assay, Severe acute respiratory syndrome-related coronavirus, Immunoprecipitation
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