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Cited by 76Animal Virus Infections StudiesSARS-CoV-2 and COVID-19 ResearchVirology and Viral Diseases

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Host cell proteins interacting with the 3′ end of TGEV coronavirus genome influence virus replication

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Galán, Carmen, Sola, Isabel, Nogales, Aitor, Thomas, Benjamin, Akoulitchev, Alexandre, Enjuanes, Luis, Almazán, Fernando·2009·Virology
DOI 10.1016/j.virol.2009.06.006PMC PMC7118768
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Abstract

Coronavirus RNA synthesis is performed by a multienzymatic replicase complex together with cellular factors. This process requires the specific recognition of RNA cis-acting signals located at the ends of the viral genome. To identify cellular proteins involved in coronavirus RNA synthesis, transmissible gastroenteritis coronavirus (TGEV) genome ends, harboring essential cis-acting signals for replication, were used as baits for RNA affinity protein purification. Ten proteins were preferentially pulled down with either the 5' or 3' ends of the genome and identified by proteomic analysis. Nine of them, including members of the heterogeneous ribonucleoprotein family of proteins (hnRNPs), the poly(A)-binding protein (PABP), the p100 transcriptional co-activator protein and two aminoacyl-tRNA synthetases, showed a preferential binding to the 3' end of the genome, whereas only the polypyrimidine tract-binding protein (PTB) was preferentially pulled down with the 5' end of the genome. The potential function of the 3' end-interacting proteins in virus replication was studied by analyzing the effect of their silencing using a TGEV-derived replicon and the infectious virus. Gene silencing of PABP, hnRNP Q, and glutamyl-prolyl-tRNA synthetase (EPRS) caused a significant 2 to 3-fold reduction of viral RNA synthesis. Interestingly, the silencing of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), initially used as a control gene, caused a 2 to 3-fold increase in viral RNA synthesis in both systems. These data suggest that PABP, hnRNP Q, and EPRS play a positive role in virus infection that could be mediated through their interaction with the viral 3' end, and that GAPDH has a negative effect on viral infection.

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  • Affinity purification-mass spectrometry approaches have been adapted to identify proteins that bind viral RNA, most often to the 5' or 3' untranslated regions thought to function in initial stages of viral RNA replication and translation.

    “AP-MS approaches have been adapted to identify proteins that bind viral RNA (vRNA), most often to the 5′ or 3′ untranslated regions thought to function in the initial stages of vRNA replication and translation (80).”

    From The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology (2014) · cites this paper as [80]

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Metadata

Authors
Galán, C., Sola, I., Nogales, A., Thomas, B., Akoulitchev, A., Enjuanes, L., & Almazán, F.
Affiliations
  • Centro Nacional de Biotecnología (ES)
  • University of Oxford (GB)
Corresponding author
Enjuanes, Luis
ORCID
Venue
Virology
Publisher
Elsevier BV
Published
2009-09
Volume
391
Issue
2
Pages
304-314
ISSN
0042-6822 (ISSN-L)
DOI
10.1016/j.virol.2009.06.006
PMC
PMC7118768
OpenAlex
W2077480910
Cited by
76
References
0
Citations by year
2026: 3 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 5 · 2021: 8 · 2020: 6 · 2019: 2 · 2018: 1 · 2017: 4 · 2016: 5 · 2015: 5 · 2014: 6 · 2012: 8
FWCI
11.78
Type
journal article
Language
en
Open access
Licence
Locations
  • Virology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Funders
  • Comunidad de Madrid (S-SAL-0185-2006)
  • Ministerio de Ciencia e Innovación
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
transmissible gastroenteritis virus, host cell proteins, poly(A)-binding protein, hnRNP Q, glutamyl-prolyl-tRNA synthetase, glyceraldehyde-3-phosphate dehydrogenase, viral replication, RNA-dependent RNA polymerase
MeSH terms
Cell Line, Chromatography, Affinity, Humans, Protein Binding, Proteins, RNA, Viral, Mass Spectrometry, Virus Replication, Coronavirus, Proteome, Hepatocytes, Host-Pathogen Interactions

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