Extensive Conservation of Prokaryotic Ribosomal Binding Sites in Known and Novel Picobirnaviruses
Open accessAbstract
Currently, the Leviviridae and Cystoviridae are the only two recognized families of prokaryotic RNA viruses. Picobirnaviruses, which are bisegmented double-stranded RNA viruses commonly found in animal stool samples, are currently thought to be animal viruses, but have not been propagated in cell culture or in an animal model. We hypothesize that picobirnaviruses are prokaryotic RNA viruses. We identified and analyzed the genomes of 38 novel picobirnaviruses and determined that a classical bacterial sequence motif, the ribosomal binding site (RBS), is present in the 5' untranslated regions (5' UTRs) of all of the novel as well as all previously published picobirnavirus sequences. Among all viruses, enrichment of the RBS motif is only observed in viral families that infect prokaryotes and not in eukaryotic infecting viral families. These results will enable future studies to more accurately understand the biology of picobirnaviruses.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
There is evidence that picobirnaviruses may actually be bacteriophage rather than eukaryote-associated viruses.
“There is, however, evidence that picobirnaviruses may actually be bacteriophage rather than eukaryote-associated viruses (Krishnamurthy and Wang 2018), such that the virology of these viruses is currently unclear.”
From Red fox viromes in urban and rural landscapes (2020) · cites this paper as Krishnamurthy and Wang 2018
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 34 · Cited by 106
Research works printed but not linked: 4
- Dabrowska, K., Switała-Jelen, K., Opolski, A., Weber-Dabrowska, B., Gorski, A., 2005. A review: bacteriophage penetration in vertebrates. J. Appl. Microbiol. 98, 7-13.
- Delmas, B., 2012. Picobirnaviridae. In: King, A.M.Q., Lefkowitz, E., Adams, M.J., Carstens, E.B. (Eds.), Virus Taxonomy, 9 ed. Elsevier, pp. 535-539.
- Górski, A., Kniotek, M., Perkowska-Ptasińska, A., Mróz, A., Przerwa, A., Gorczyca, W., Dabrowska, K., Weber-Dabrowska, B., Nowaczyk, M., 2006. Bacteriophages and transplantation tolerance. Transplant. Proc. 38, 331-333.
- Shine, J., Dalgarno, L., 1974. The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA 71, 1342-1346.
- Authors
- Krishnamurthy, S. R., & Wang, D.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Wang, David
- Venue
- Virology
- Publisher
- Elsevier BV
- Published
- 2018-03
- Volume
- 516
- Pages
- 108-114
- ISSN
- 0042-6822 (ISSN-L)
- OpenAlex
- W2783479211
- Cited by
- 106
- References
- 34
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 7 · 2023: 12 · 2022: 18 · 2021: 21 · 2020: 15 · 2019: 9 · 2018: 13
- FWCI
- 10.91
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Burroughs Wellcome Fund
- Topics
- Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Plant and Fungal Interactions Research
- Keywords
- ribosome binding site, 5' untranslated region, Cystoviridae, viral genome analysis, viral evolution
- MeSH terms
- Animals, Bacteria, Base Sequence, Binding Sites, Codon, Molecular Sequence Data, Ribosomes, Conserved Sequence, Picobirnavirus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
